LOCUS       BBa_J24799              2070 bp    DNA     linear       08-May-2024
DEFINITION  Arsenic promoter + LuxI + GFP
ACCESSION   BBa_J24799
VERSION     BBa_J24799.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     misc_feature    531..534
                     /label=conserved
     RBS             527..538
                     /label=BBa_B0034
     misc_feature    1124..1155
                     /label=LVA
     CDS             545..1123
                     /label=luxI
     misc_feature    545..1162
                     /label=BBa_C0061
     misc_feature    1163..1187
                     /label=Help:Barcodes
     CDS             545..1162
                     /label=BBa_C0061
     misc_feature    527..1187
                     /label=BBa_C0261
     misc_feature    1200..1203
                     /label=conserved
     RBS             1196..1207
                     /label=BBa_B0034
     CDS             1214..1933
                     /label=GFP protein
     CDS             1214..1933
                     /label=BBa_E0040
     stem_loop       1953..1996
                     /label=stem_loop
     misc_feature    1942..2021
                     /label=BBa_B0010
     terminator      1942..2021
                     /label=BBa_B0010
     stem_loop       2037..2056
                     /label=T7 TE
     misc_feature    2063..2063
                     /label=stop
     polyA_site      2057..2070
                     /label=polya
     misc_feature    2030..2070
                     /label=BBa_B0012
     terminator      2030..2070
                     /label=BBa_B0012
     misc_feature    1196..2070
                     /label=BBa_I13504
     misc_feature    527..2070
                     /label=BBa_J37034
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagaaag aggagaaata
      541 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa
      601 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt
      661 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg
      721 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat
      781 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat
      841 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag
      901 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac
      961 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc
     1021 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt
     1081 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa
     1141 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaaga
     1201 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1261 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1321 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1381 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1441 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1501 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1561 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1621 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1681 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1741 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1801 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1861 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1921 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1981 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2041 caccttcggg tgggcctttc tgcgtttata
//