LOCUS       BBa_J25010               994 bp    DNA     linear       22-Nov-2024
DEFINITION  mCherry (+lva) controlled by OmpR (good reporter w/ photosystem)
ACCESSION   BBa_J25010
VERSION     BBa_J25010.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..842
                     /label=mCherry
     misc_feature    486..486
                     /label=C->T (removing PstI site)
     misc_feature    843..851
                     /label=LVA
     misc_feature    135..857
                     /label=BBa_J06505
     stem_loop       877..920
                     /label=stem_loop
     misc_feature    866..945
                     /label=BBa_B0010
     terminator      866..945
                     /label=BBa_B0010
     stem_loop       961..980
                     /label=T7 TE
     misc_feature    987..987
                     /label=stop
     polyA_site      981..994
                     /label=polya
     misc_feature    954..994
                     /label=BBa_B0012
     terminator      954..994
                     /label=BBa_B0012
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
      181 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      241 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      301 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      361 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      421 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      481 cctccttgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      541 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      601 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      661 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      721 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      781 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      841 agttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      901 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      961 ggctcacctt cgggtgggcc tttctgcgtt tata
//