##gff-version 3
##sequence-region BBa_J25010 1 994
BBa_J25010	.	terminator	954	994	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J25010	.	polyA_site	981	994	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J25010	.	stop_codon	987	987	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J25010	.	engineered_region	954	994	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J25010	.	stem_loop	961	980	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J25010	.	engineered_region	135	857	.	+	0	ID=BBa_J06505;Name=BBa_J06505
BBa_J25010	.	CDS	135	842	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J25010	.	sequence_alteration	486	486	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J25010	.	tag	843	851	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J25010	.	terminator	866	945	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J25010	.	engineered_region	866	945	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J25010	.	stem_loop	877	920	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J25010	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J25010	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25010	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_J25010	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J25010	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J25010	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J25010	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J25010	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
>BBa_J25010
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtgagcaagggcgaggaggat
aacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcg
agggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgccctt
cgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactac
ttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgaccc
aggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgt
aatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatc
aagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgc
agctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagta
cgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata