LOCUS BBa_J25011 5043 bp DNA linear 14-May-2024 DEFINITION Photosensing system, expresses mCherry ACCESSION BBa_J25011 VERSION BBa_J25011.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 790..814 /label=Help:Barcodes CDS 64..814 /label=BBa_I15008 misc_feature 827..830 /label=conserved RBS 823..834 /label=BBa_B0034 CDS 841..1590 /label=PcyA misc_feature 1591..1615 /label=Help:Barcodes CDS 841..1615 /label=BBa_I15009 misc_feature 1628..1631 /label=conserved RBS 1624..1635 /label=BBa_B0034 CDS 1642..3879 /label=Cph8 misc_feature 3880..3904 /label=Help:Barcodes CDS 1642..3904 /label=BBa_I15010 stem_loop 3924..3967 /label=stem_loop misc_feature 3913..3992 /label=BBa_B0010 terminator 3913..3992 /label=BBa_B0010 stem_loop 4008..4027 /label=T7 TE misc_feature 4034..4034 /label=stop polyA_site 4028..4041 /label=polya misc_feature 4001..4041 /label=BBa_B0012 terminator 4001..4041 /label=BBa_B0012 terminator 3913..4041 /label=BBa_B0015 misc_feature 4062..4079 /label=C1 OmpR misc_feature 4083..4100 /label=C2 OmpR misc_feature 4103..4120 /label=C3 OmpR promoter 4124..4129 /label=-35 promoter 4147..4152 /label=-10 promoter 4050..4157 /label=BBa_R0082 misc_feature 4170..4173 /label=conserved RBS 4166..4177 /label=BBa_B0034 CDS 4184..4891 /label=mCherry misc_feature 4535..4535 /label=C->T (removing PstI site) misc_feature 4892..4900 /label=LVA misc_feature 4184..4906 /label=BBa_J06505 stem_loop 4926..4969 /label=stem_loop misc_feature 4915..4994 /label=BBa_B0010 terminator 4915..4994 /label=BBa_B0010 stem_loop 5010..5029 /label=T7 TE misc_feature 5036..5036 /label=stop polyA_site 5030..5043 /label=polya misc_feature 5003..5043 /label=BBa_B0012 terminator 5003..5043 /label=BBa_B0012 terminator 4915..5043 /label=BBa_B0015 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgagtg tcaacttagc ttcccagttg cgggaaggga cgaaaaaatc ccactccatg 121 gcggagaacg tcggctttgt caaatgcttc ctcaagggcg ttgtcgagaa aaattcctac 181 cgtaagctgg ttggcaatct ctactttgtc tacagtgcca tggaagagga aatggcaaaa 241 tttaaggacc atcccatcct cagccacatt tacttccccg aactcaaccg caaacaaagc 301 ctagagcaag acctgcaatt ctattacggc tccaactggc ggcaagaagt gaaaatttct 361 gccgctggcc aagcctatgt ggaccgagtc cggcaagtgg ccgctacggc ccctgaattg 421 ttggtggccc attcctacac ccgttacctg ggggatcttt ccggcggtca aattctcaag 481 aaaattgccc aaaatgccat gaatctccac gatggtggca cagctttcta tgaatttgcc 541 gacattgatg acgaaaaggc ttttaaaaat acctaccgtc aagctatgaa tgatctgccc 601 attgaccaag ccaccgccga acggattgtg gatgaagcca atgacgcctt tgccatgaac 661 atgaaaatgt tcaacgaact tgaaggcaac ctgatcaagg cgatcggcat tatggtgttc 721 aacagcctca cccgtcgccg cagtcaaggc agcaccgaag ttggcctcgc cacctccgaa 781 ggctaataac gctgatagtg ctagtgtaga tcgctactag agaaagagga gaaatactag 841 atggccgtca ctgatttaag tttgaccaat tcttccctga tgcctacgtt gaacccgatg 901 attcaacagt tggccctggc gatcgccgct agttggcaaa gtttacccct caagccctat 961 caattgccgg aggatttggg ctacgtagaa ggccgcctgg aaggggaaaa gttagtgatt 1021 gaaaatcggt gctaccaaac gccccagttt cgcaaaatgc atttggagtt ggccaaggtg 1081 ggcaaagggt tggatattct ccactgtgta atgtttcctg agcctttata cggtctacct 1141 ttgtttggct gtgacattgt ggccggcccc ggtggagtaa gtgcggctat tgcggatcta 1201 tcccccaccc aaagcgatcg ccaattgccc gcagcgtacc aaaaatcatt ggcagagcta 1261 ggccagccag aatttgagca acaacgggaa ttgcccccct ggggagaaat attttctgaa 1321 tattgtttat tcatccgtcc cagcaatgtc actgaagaag aaagatttgt acaaagggta 1381 gtggactttt tgcaaattca ttgtcaccaa tccatcgttg ccgaaccctt gtctgaagct 1441 caaactttgg agcaccgtca ggggcaaatt cattactgcc aacaacaaca gaaaaatgat 1501 aaaacccgtc gggtactgga aaaagctttt ggggaagctt gggcggaacg gtatatgagc 1561 caagtcttat ttgatgttat ccaataataa ctctgatagt gctagtgtag atctctacta 1621 gagaaagagg agaaatacta gatggccacc accgtacaac tcagcgacca atccctccgt 1681 cagctagaaa ccctcgccat ccacaccgcc cacctgattc agccccacgg tttagtggtg 1741 gtcctgcaag aaccagacct caccatcagc caaattagcg ccaactgcac cggcatttta 1801 gggcgatcgc cagaggattt gttgggcaga accctagggg aagtgtttga tagctttcag 1861 attgatccca tccagagtcg cctaacggcc ggacaaatca gcagcctcaa ccccagtaaa 1921 ctttgggcgc gggtcatggg ggacgacttt gtcatttttg acggggtttt tcatcgcaac 1981 agtgacggtt tattggtatg tgaactcgag ccagcctaca cttccgataa tctgcccttc 2041 ctcggttttt atcacatggc caacgctgcc ctgaatcggt tgcgccaaca agctaatcta 2101 cgggatttct acgatgttat tgtcgaagaa gtccgccgta tgactggctt tgaccgggtg 2161 atgctatacc gctttgatga aaataaccac ggtgatgtca ttgccgaaga taaacgggat 2221 gatatggaac cctatttggg cctgcactat cccgaatcgg atattcccca acccgcccgt 2281 cggctattta tccacaaccc cattcgagta attcccgatg tttatggtgt ggcggtgccc 2341 ctgaccccag cggttaaccc cagcaccaac cgagcggtgg atttaacaga atccattctg 2401 cgcagtgcgt accattgcca cttgacctat ctgaaaaata tgggggtagg agcgtcttta 2461 accatttccc taattaagga cggccatctc tgggggctca ttgcctgcca ccatcaaacc 2521 cccaaagtaa ttccctttga actgcgtaaa gcctgcgaat tttttggtcg ggtggtgttt 2581 agcaacattt ccgcccagga agatacggaa accttcgatt accgggtgca gctggcggag 2641 catgaagcgg ttttattgga caaaatgacc acggcggcgg attttgtcga aggattaact 2701 aatcatcccg atcgcctgtt gggattaacg ggctcccagg gggcggccat ttgctttggg 2761 gaaaaattga ttttagtagg ggaaaccccg gacgagaaag cagtgcaata tttactgcaa 2821 tggttggaga atcgggaagt gcaagacgtt ttcttcacct cttccctctc acaaatttat 2881 cctgatgcag tgaattttaa atccgtggcc agtggcttat tggccattcc cattgcccgt 2941 cacaactttt tgctctggtt tcgccctgaa gtgttgcaaa cggttaattg gggcggtgac 3001 ccaaatcatg cttacgaagc tacccaggaa gacggtaaaa tcgagctcca tccccgccaa 3061 tcctttgacc tctggaaaga aattgtccga ctccaatctt tgccctggca atcggtggaa 3121 atccaaagtg ccctggccct gaaaaaggcg atcgtcaacc tcattttgcg ccaggcagaa 3181 gaattgcata tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg 3241 ggggtaagtc acgacttgcg cacgccgctg acgcgtattc gcctggcgac tgagatgatg 3301 agcgagcagg atggctatct ggcagaatcg atcaataaag atatcgaaga gtgcaacgcc 3361 atcattgagc agtttatcga ctacctgcgc accgggcagg agatgccgat ggaaatggcg 3421 gatcttaatg cagtactcgg tgaggtgatt gctgccgaaa gtggctatga gcgggaaatt 3481 gaaaccgcgc tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc 3541 gcggtggcga atatggtggt caacgccgcc cgttatggca atggctgggt caaagtcagc 3601 agcggaacgg agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg 3661 ccggaacaac gtaagcacct gttccagccg tttgtccgcg gcgacagtgc gcgcaccatt 3721 agcggcacgg gattagggct ggcaattgtg cagcgtatcg tggataacca taacgggatg 3781 ctggagcttg gcaccagcga gcggggcggg ctttccattc gcgcctggct gccagtgccg 3841 gtaacgcggg cgcagggcat gacaaaagaa gggtaataac tctgatagtg ctagtgtaga 3901 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3961 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 4021 gtgggccttt ctgcgtttat atactagagt cccttgcatt tacattttga aacatctata 4081 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt 4141 tttggggaga atggacttac tagagaaaga ggagaaatac tagatggtga gcaagggcga 4201 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 4261 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 4321 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 4381 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 4441 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 4501 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa 4561 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 4621 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 4681 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 4741 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 4801 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 4861 ctccaccggc ggcatggacg agctgtacaa gttagtagct taataatact agagccaggc 4921 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 4981 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 5041 ata //