##gff-version 3
##sequence-region BBa_J25011 1 5043
BBa_J25011	.	engineered_region	4184	4906	.	+	0	ID=BBa_J06505;Name=BBa_J06505
BBa_J25011	.	tag	4892	4900	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J25011	.	sequence_alteration	4535	4535	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J25011	.	CDS	4184	4891	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J25011	.	ribosome_entry_site	4166	4177	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J25011	.	conserved_region	4170	4173	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25011	.	CDS	841	1615	.	+	0	ID=BBa_I15009;Name=BBa_I15009
BBa_J25011	.	CDS	841	1590	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_J25011	.	engineered_tag	1591	1615	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_J25011	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J25011	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25011	.	terminator	4915	5043	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J25011	.	terminator	4915	4994	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J25011	.	engineered_region	4915	4994	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J25011	.	stem_loop	4926	4969	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J25011	.	terminator	5003	5043	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J25011	.	polyA_site	5030	5043	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J25011	.	stem_loop	5010	5029	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J25011	.	engineered_region	5003	5043	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J25011	.	stop_codon	5036	5036	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J25011	.	CDS	1642	3904	.	+	0	ID=BBa_I15010;Name=BBa_I15010
BBa_J25011	.	CDS	1642	3879	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_J25011	.	engineered_tag	3880	3904	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_J25011	.	promoter	4050	4157	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_J25011	.	non_covalent_binding_site	4103	4120	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J25011	.	non_covalent_binding_site	4062	4079	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J25011	.	promoter	4124	4129	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J25011	.	promoter	4147	4152	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J25011	.	non_covalent_binding_site	4083	4100	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J25011	.	ribosome_entry_site	823	834	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J25011	.	conserved_region	827	830	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25011	.	ribosome_entry_site	1624	1635	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J25011	.	conserved_region	1628	1631	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25011	.	terminator	3913	4041	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J25011	.	terminator	3913	3992	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J25011	.	engineered_region	3913	3992	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J25011	.	stem_loop	3924	3967	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J25011	.	terminator	4001	4041	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J25011	.	polyA_site	4028	4041	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J25011	.	stem_loop	4008	4027	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J25011	.	engineered_region	4001	4041	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J25011	.	stop_codon	4034	4034	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J25011	.	CDS	64	814	.	+	0	ID=BBa_I15008;Name=BBa_I15008
BBa_J25011	.	engineered_tag	790	814	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_J25011	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_J25011	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J25011	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J25011	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
>BBa_J25011
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgagtgtcaacttag
cttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaaggg
cgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggca
aaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgc
aattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggca
agtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaatt
ctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacg
aaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtgga
tgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcatt
atggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataac
gctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaa
ttcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttaccc
ctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatc
ggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactg
tgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcg
gctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggcc
agccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccag
caatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgcc
gaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgata
aaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttat
ccaataataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggccaccaccgtacaa
ctcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtgg
tggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagagga
tttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaa
atcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatc
gcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatca
catggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtc
cgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagata
aacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttat
ccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaac
cgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtag
gagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagt
aattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacg
gaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttg
tcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaa
attgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaa
gacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattgg
ccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgaccc
aaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaa
attgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacc
tcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggc
gggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctat
ctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggc
aggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcggga
aattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatg
gtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccagg
tggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcg
caccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggc
accagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaag
ggtaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagtttt
agtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgg
tgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaa
cggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgacc
aagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcacc
ccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacgg
cggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaac
ttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacg
gcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccaccta
caaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggac
tacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtag
cttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata