##gff-version 3
##sequence-region BBa_J25500 1 5335
BBa_J25500	.	engineered_region	1	4333	.	+	0	ID=BBa_M30109;Name=BBa_M30109
BBa_J25500	.	CDS	1934	4171	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_M30109
BBa_J25500	.	CDS	1934	4171	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_J25500	.	engineered_tag	4172	4196	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_J25500	.	CDS	934	1683	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_M30109
BBa_J25500	.	CDS	934	1683	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_J25500	.	engineered_tag	1684	1708	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_J25500	.	terminator	4205	4284	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_J25500	.	engineered_region	4205	4284	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J25500	.	stem_loop	4216	4259	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J25500	.	terminator	1805	1845	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_J25500	.	engineered_region	1805	1845	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J25500	.	stop_codon	1838	1838	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J25500	.	stem_loop	1812	1831	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J25500	.	polyA_site	1832	1845	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J25500	.	ribosome_entry_site	1916	1927	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_J25500	.	conserved_region	1920	1923	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25500	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_M30109
BBa_J25500	.	CDS	157	882	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_M30109
BBa_J25500	.	engineered_tag	883	907	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_J25500	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_J25500	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25500	.	terminator	1717	1796	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_J25500	.	engineered_region	1717	1796	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J25500	.	stem_loop	1728	1771	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J25500	.	ribosome_entry_site	916	927	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_J25500	.	conserved_region	920	923	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25500	.	terminator	4293	4333	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_J25500	.	engineered_region	4293	4333	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J25500	.	stop_codon	4326	4326	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J25500	.	stem_loop	4300	4319	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J25500	.	polyA_site	4320	4333	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J25500	.	promoter	1854	1907	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_M30109
BBa_J25500	.	promoter	1873	1878	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J25500	.	non_covalent_binding_site	1879	1897	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J25500	.	engineered_region	1854	1907	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J25500	.	promoter	1896	1901	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J25500	.	non_covalent_binding_site	1854	1872	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J25500	.	sequence_feature	4342	5335	.	+	0	ID=BBa_J25010;Name=BBa_J25010
BBa_J25500	.	terminator	5207	5286	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J25010
BBa_J25500	.	engineered_region	5207	5286	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J25500	.	stem_loop	5218	5261	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J25500	.	ribosome_entry_site	4458	4469	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J25010
BBa_J25500	.	conserved_region	4462	4465	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J25500	.	terminator	5295	5335	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J25010
BBa_J25500	.	engineered_region	5295	5335	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J25500	.	stop_codon	5328	5328	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J25500	.	stem_loop	5302	5321	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J25500	.	polyA_site	5322	5335	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J25500	.	promoter	4342	4449	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_J25010
BBa_J25500	.	promoter	4439	4444	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J25500	.	promoter	4416	4421	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J25500	.	non_covalent_binding_site	4354	4371	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J25500	.	non_covalent_binding_site	4375	4392	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J25500	.	non_covalent_binding_site	4395	4412	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J25500	.	engineered_region	4476	5198	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J25010
BBa_J25500	.	CDS	4476	5183	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J25500	.	sequence_alteration	4827	4827	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J25500	.	tag	5184	5192	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
>BBa_J25500
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaa
tgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatgg
aagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcct
agagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtg
gaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatcttt
ccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgc
cgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgcc
gaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatca
aggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctc
cgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgat
ttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagtt
ggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagtt
agtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttg
gatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccg
gtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcatt
ggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgttta
ttcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcacc
aatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaaca
acagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtc
ttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagag
atactgagcactactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcag
ctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctca
ccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctagggga
agtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaa
ctttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtat
gtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcg
gttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgg
gtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatt
tgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcc
cgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatcc
attctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaa
ttaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagc
ctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcag
ctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccg
atcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaacccc
ggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctc
tcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaact
ttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctaccca
ggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccc
tggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaat
tgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcac
gccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagat
atcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcgg
atcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccgg
cagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggc
aatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattg
cgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagg
gctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttcc
attcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagt
gtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccc
ttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattat
tctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataaca
tggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgaggg
cgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcc
tgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttga
agctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccagga
ctcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatg
cagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagc
agaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagct
gcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaa
cgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata