BBa_J26008
type: ComponentDefinition
persistent identity: BBa J26008
display id: BBa_J26008
version: 1
was generated by: iGEM to SBOL conversion
description: makes aspartate and luxI
created: 2006-08-07T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: testing
owned by: james
owned by: myers
mutable description: another test part
discontinued: false
dominant: false
group u list: _77_
m user id: 0
owner id: 776
owning group id: 77
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Phillipa Sessini
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_C0083
component: BBa_B0034
component: BBa_F1610
sequence annotation: BBa_B0034
sequence annotation: BBa_C0083
sequence annotation: BBa_F1610
sequence: https://synbiohub.org/public/igem/BBa_J26008_sequence/1

BBa_C0083
type: Component
persistent identity: component2260084
display id: component2260084
version: 1
top level: BBa_J26008
definition: aspA
access: public

BBa_B0034
type: Component
persistent identity: component2260075
display id: component2260075
version: 1
top level: BBa_J26008
definition: BBa_B0034
access: public

BBa_F1610
type: Component
persistent identity: component2260097
display id: component2260097
version: 1
top level: BBa_J26008
definition: BBa_F1610
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2260075
display id: annotation2260075
version: 1
top level: BBa_J26008
location: https://synbiohub.org/public/igem/BBa_J26008/annotation2260075/range2260075/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J26008/annotation2260075/range2260075/1
type: Range
persistent identity: range2260075
display id: range2260075
version: 1
top level: BBa_J26008
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0083
type: SequenceAnnotation
persistent identity: annotation2260084
display id: annotation2260084
version: 1
top level: BBa_J26008
location: https://synbiohub.org/public/igem/BBa_J26008/annotation2260084/range2260084/1
component: BBa_C0083

https://synbiohub.org/public/igem/BBa_J26008/annotation2260084/range2260084/1
type: Range
persistent identity: range2260084
display id: range2260084
version: 1
top level: BBa_J26008
direction: direction/reverse
start: 19
end: 1561
orientation: inline

BBa_F1610
type: SequenceAnnotation
persistent identity: annotation2260097
display id: annotation2260097
version: 1
top level: BBa_J26008
location: https://synbiohub.org/public/igem/BBa_J26008/annotation2260097/range2260097/1
component: BBa_F1610

https://synbiohub.org/public/igem/BBa_J26008/annotation2260097/range2260097/1
type: Range
persistent identity: range2260097
display id: range2260097
version: 1
top level: BBa_J26008
direction: direction/reverse
start: 1570
end: 2367
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_F1610
type: ComponentDefinition
persistent identity: BBa F1610
display id: BBa_F1610
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC6HSL Sender Device
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: Formerly I0461
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_B0034
component: BBa_B0010
component: BBa_C0061
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_F1610_sequence/1

BBa_B0034
type: Component
persistent identity: component943133
display id: component943133
version: 1
top level: BBa_F1610
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943150
display id: component943150
version: 1
top level: BBa_F1610
definition: BBa_B0010
access: public

BBa_C0061
type: Component
persistent identity: component943143
display id: component943143
version: 1
top level: BBa_F1610
definition: luxI
access: public

BBa_B0012
type: Component
persistent identity: component943160
display id: component943160
version: 1
top level: BBa_F1610
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943133
display id: annotation943133
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
type: Range
persistent identity: range943133
display id: range943133
version: 1
top level: BBa_F1610
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation943143
display id: annotation943143
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
type: Range
persistent identity: range943143
display id: range943143
version: 1
top level: BBa_F1610
direction: direction/forward
start: 19
end: 636
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943150
display id: annotation943150
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
type: Range
persistent identity: range943150
display id: range943150
version: 1
top level: BBa_F1610
direction: direction/forward
start: 670
end: 749
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943160
display id: annotation943160
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
type: Range
persistent identity: range943160
display id: range943160
version: 1
top level: BBa_F1610
direction: direction/forward
start: 758
end: 798
orientation: inline

aspA
type: ComponentDefinition
persistent identity: BBa C0083
display id: BBa_C0083
version: 1
was generated by: iGEM to SBOL conversion
description: aspartate ammonia-lyase
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: AE000486 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The start codon was changed from GTG to ATG to match BioBricks standards. Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AspA
sequence annotation: G
sequence annotation: C
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0083_sequence/1

AspA
type: SequenceAnnotation
persistent identity: annotation300981
display id: annotation300981
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
type: Range
persistent identity: range300981
display id: range300981
version: 1
top level: aspA
direction: direction/forward
start: 1
end: 1479
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300982
display id: annotation300982
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
type: Range
persistent identity: range300982
display id: range300982
version: 1
top level: aspA
direction: direction/forward
start: 1
end: 1
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300983
display id: annotation300983
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
type: Range
persistent identity: range300983
display id: range300983
version: 1
top level: aspA
direction: direction/forward
start: 588
end: 588
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300984
display id: annotation300984
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
type: Range
persistent identity: range300984
display id: range300984
version: 1
top level: aspA
direction: direction/forward
start: 624
end: 624
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300986
display id: annotation300986
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
type: Range
persistent identity: range300986
display id: range300986
version: 1
top level: aspA
direction: direction/forward
start: 657
end: 657
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300987
display id: annotation300987
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
type: Range
persistent identity: range300987
display id: range300987
version: 1
top level: aspA
direction: direction/forward
start: 999
end: 999
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300988
display id: annotation300988
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
type: Range
persistent identity: range300988
display id: range300988
version: 1
top level: aspA
direction: direction/forward
start: 1125
end: 1125
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation300993
display id: annotation300993
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
type: Range
persistent identity: range300993
display id: range300993
version: 1
top level: aspA
direction: direction/forward
start: 1480
end: 1512
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213998
display id: annotation2213998
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
type: Range
persistent identity: range2213998
display id: range2213998
version: 1
top level: aspA
direction: direction/forward
start: 1519
end: 1543
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_J26008_sequence/1
type: Sequence
persistent identity: BBa J26008 sequence
display id: BBa_J26008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactgttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcgaatccgttcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtgtgcgaaggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcgtgggtaaaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaacttgacgatattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_F1610_sequence/1
type: Sequence
persistent identity: BBa F1610 sequence
display id: BBa_F1610_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0083_sequence/1
type: Sequence
persistent identity: BBa C0083 sequence
display id: BBa_C0083_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactgttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcgaatccgttcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtgtgcgaaggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcgtgggtaaaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaacttgacgatattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z