##gff-version 3
##sequence-region BBa_J26011 1 4112
BBa_J26011	.	engineered_region	1	1570	.	+	0	ID=BBa_S03569;Name=BBa_S03569
BBa_J26011	.	engineered_region	1	769	.	+	0	ID=BBa_S03321;Name=BBa_S03321;Parent=BBa_S03569
BBa_J26011	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03321
BBa_J26011	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J26011	.	CDS	19	744	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_S03321
BBa_J26011	.	engineered_tag	745	769	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_J26011	.	engineered_region	778	1570	.	+	0	ID=BBa_S03322;Name=BBa_S03322;Parent=BBa_S03569
BBa_J26011	.	ribosome_entry_site	778	789	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03322
BBa_J26011	.	conserved_region	782	785	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J26011	.	CDS	796	1545	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_S03322
BBa_J26011	.	CDS	796	1545	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_J26011	.	engineered_tag	1546	1570	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_J26011	.	engineered_region	1579	4112	.	+	0	ID=BBa_S03570;Name=BBa_S03570
BBa_J26011	.	promoter	4005	4112	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_S03570
BBa_J26011	.	promoter	4079	4084	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J26011	.	promoter	4102	4107	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J26011	.	non_covalent_binding_site	4058	4075	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J26011	.	non_covalent_binding_site	4017	4034	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J26011	.	non_covalent_binding_site	4038	4055	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J26011	.	engineered_region	1579	3996	.	+	0	ID=BBa_S03417;Name=BBa_S03417;Parent=BBa_S03570
BBa_J26011	.	ribosome_entry_site	1579	1590	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03417
BBa_J26011	.	conserved_region	1583	1586	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J26011	.	terminator	3956	3996	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03417
BBa_J26011	.	polyA_site	3983	3996	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J26011	.	engineered_region	3956	3996	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J26011	.	stem_loop	3963	3982	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J26011	.	stop_codon	3989	3989	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J26011	.	terminator	3868	3947	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03417
BBa_J26011	.	stem_loop	3879	3922	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J26011	.	engineered_region	3868	3947	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J26011	.	CDS	1597	3834	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_S03417
BBa_J26011	.	engineered_tag	3835	3859	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_J26011	.	CDS	1597	3834	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
>BBa_J26011
aaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcgg
agaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctcta
ctttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaa
ctcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctg
ccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacac
ccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggc
acagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgc
ccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacga
acttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcacc
gaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaat
actagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggc
cctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggc
cgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttgg
ccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctg
tgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgccc
gcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaa
tattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactt
tttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaa
attcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcgg
aacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagaa
agaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccac
accgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgcca
actgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagat
tgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggg
gacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctaca
cttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatct
acgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgat
gaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaat
cggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggt
gcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccat
tgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctggg
ggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggt
ggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggtt
ttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgg
gctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaata
tttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgca
gtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctg
aagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagct
ccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaa
agtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgtta
agcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcct
ggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatc
attgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtg
aggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgca
cccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagc
agcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacc
tgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtat
cgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtg
ccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaaca
tctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatg
gact