LOCUS BBa_J26100 6549 bp DNA linear 31-Oct-2024 DEFINITION Light -> Aspartate + AHSL ACCESSION BBa_J26100 VERSION BBa_J26100.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 807..831 /label=Help:Barcodes CDS 81..806 /label=BBa_I15008 misc_feature 63..831 /label=BBa_S03321 misc_feature 844..847 /label=conserved RBS 840..851 /label=BBa_B0034 CDS 858..1607 /label=PcyA misc_feature 1608..1632 /label=Help:Barcodes CDS 858..1607 /label=BBa_I15009 misc_feature 840..1632 /label=BBa_S03322 misc_feature 1645..1648 /label=conserved RBS 1641..1652 /label=BBa_B0034 CDS 1659..3896 /label=Cph8 misc_feature 3897..3921 /label=Help:Barcodes CDS 1659..3896 /label=BBa_I15010 stem_loop 3941..3984 /label=stem_loop misc_feature 3930..4009 /label=BBa_B0010 terminator 3930..4009 /label=BBa_B0010 stem_loop 4025..4044 /label=T7 TE misc_feature 4051..4051 /label=stop polyA_site 4045..4058 /label=polya misc_feature 4018..4058 /label=BBa_B0012 terminator 4018..4058 /label=BBa_B0012 misc_feature 1641..4058 /label=BBa_S03417 misc_feature 4079..4096 /label=C1 OmpR misc_feature 4100..4117 /label=C2 OmpR misc_feature 4120..4137 /label=C3 OmpR promoter 4141..4146 /label=-35 promoter 4164..4169 /label=-10 promoter 4067..4174 /label=BBa_R0082 misc_feature 1..4174 /label=BBa_J26007 misc_feature 4187..4190 /label=conserved RBS 4183..4194 /label=BBa_B0034 CDS 4201..5679 /label=AspA misc_feature 4201..4201 /label=G misc_feature 4788..4788 /label=C misc_feature 4824..4824 /label=G misc_feature 4857..4857 /label=C misc_feature 5199..5199 /label=G misc_feature 5325..5325 /label=G misc_feature 5680..5712 /label=LVA misc_feature 5719..5743 /label=Help:Barcodes CDS 4201..5743 /label=BBa_C0083 misc_feature 5756..5759 /label=conserved RBS 5752..5763 /label=BBa_B0034 misc_feature 6349..6380 /label=LVA CDS 5770..6348 /label=luxI misc_feature 5770..6387 /label=BBa_C0061 misc_feature 6388..6412 /label=Help:Barcodes CDS 5770..6387 /label=BBa_C0061 stem_loop 6432..6475 /label=stem_loop misc_feature 6421..6500 /label=BBa_B0010 terminator 6421..6500 /label=BBa_B0010 stem_loop 6516..6535 /label=T7 TE misc_feature 6542..6542 /label=stop polyA_site 6536..6549 /label=polya misc_feature 6509..6549 /label=BBa_B0012 terminator 6509..6549 /label=BBa_B0012 misc_feature 5752..6549 /label=BBa_F1610 misc_feature 4183..6549 /label=BBa_J26008 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagtgtca acttagcttc ccagttgcgg gaagggacga 121 aaaaatccca ctccatggcg gagaacgtcg gctttgtcaa atgcttcctc aagggcgttg 181 tcgagaaaaa ttcctaccgt aagctggttg gcaatctcta ctttgtctac agtgccatgg 241 aagaggaaat ggcaaaattt aaggaccatc ccatcctcag ccacatttac ttccccgaac 301 tcaaccgcaa acaaagccta gagcaagacc tgcaattcta ttacggctcc aactggcggc 361 aagaagtgaa aatttctgcc gctggccaag cctatgtgga ccgagtccgg caagtggccg 421 ctacggcccc tgaattgttg gtggcccatt cctacacccg ttacctgggg gatctttccg 481 gcggtcaaat tctcaagaaa attgcccaaa atgccatgaa tctccacgat ggtggcacag 541 ctttctatga atttgccgac attgatgacg aaaaggcttt taaaaatacc taccgtcaag 601 ctatgaatga tctgcccatt gaccaagcca ccgccgaacg gattgtggat gaagccaatg 661 acgcctttgc catgaacatg aaaatgttca acgaacttga aggcaacctg atcaaggcga 721 tcggcattat ggtgttcaac agcctcaccc gtcgccgcag tcaaggcagc accgaagttg 781 gcctcgccac ctccgaaggc taataacgct gatagtgcta gtgtagatcg ctactagaga 841 aagaggagaa atactagatg gccgtcactg atttaagttt gaccaattct tccctgatgc 901 ctacgttgaa cccgatgatt caacagttgg ccctggcgat cgccgctagt tggcaaagtt 961 tacccctcaa gccctatcaa ttgccggagg atttgggcta cgtagaaggc cgcctggaag 1021 gggaaaagtt agtgattgaa aatcggtgct accaaacgcc ccagtttcgc aaaatgcatt 1081 tggagttggc caaggtgggc aaagggttgg atattctcca ctgtgtaatg tttcctgagc 1141 ctttatacgg tctacctttg tttggctgtg acattgtggc cggccccggt ggagtaagtg 1201 cggctattgc ggatctatcc cccacccaaa gcgatcgcca attgcccgca gcgtaccaaa 1261 aatcattggc agagctaggc cagccagaat ttgagcaaca acgggaattg cccccctggg 1321 gagaaatatt ttctgaatat tgtttattca tccgtcccag caatgtcact gaagaagaaa 1381 gatttgtaca aagggtagtg gactttttgc aaattcattg tcaccaatcc atcgttgccg 1441 aacccttgtc tgaagctcaa actttggagc accgtcaggg gcaaattcat tactgccaac 1501 aacaacagaa aaatgataaa acccgtcggg tactggaaaa agcttttggg gaagcttggg 1561 cggaacggta tatgagccaa gtcttatttg atgttatcca ataataactc tgatagtgct 1621 agtgtagatc tctactagag aaagaggaga aatactagat ggccaccacc gtacaactca 1681 gcgaccaatc cctccgtcag ctagaaaccc tcgccatcca caccgcccac ctgattcagc 1741 cccacggttt agtggtggtc ctgcaagaac cagacctcac catcagccaa attagcgcca 1801 actgcaccgg cattttaggg cgatcgccag aggatttgtt gggcagaacc ctaggggaag 1861 tgtttgatag ctttcagatt gatcccatcc agagtcgcct aacggccgga caaatcagca 1921 gcctcaaccc cagtaaactt tgggcgcggg tcatggggga cgactttgtc atttttgacg 1981 gggtttttca tcgcaacagt gacggtttat tggtatgtga actcgagcca gcctacactt 2041 ccgataatct gcccttcctc ggtttttatc acatggccaa cgctgccctg aatcggttgc 2101 gccaacaagc taatctacgg gatttctacg atgttattgt cgaagaagtc cgccgtatga 2161 ctggctttga ccgggtgatg ctataccgct ttgatgaaaa taaccacggt gatgtcattg 2221 ccgaagataa acgggatgat atggaaccct atttgggcct gcactatccc gaatcggata 2281 ttccccaacc cgcccgtcgg ctatttatcc acaaccccat tcgagtaatt cccgatgttt 2341 atggtgtggc ggtgcccctg accccagcgg ttaaccccag caccaaccga gcggtggatt 2401 taacagaatc cattctgcgc agtgcgtacc attgccactt gacctatctg aaaaatatgg 2461 gggtaggagc gtctttaacc atttccctaa ttaaggacgg ccatctctgg gggctcattg 2521 cctgccacca tcaaaccccc aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt 2581 ttggtcgggt ggtgtttagc aacatttccg cccaggaaga tacggaaacc ttcgattacc 2641 gggtgcagct ggcggagcat gaagcggttt tattggacaa aatgaccacg gcggcggatt 2701 ttgtcgaagg attaactaat catcccgatc gcctgttggg attaacgggc tcccaggggg 2761 cggccatttg ctttggggaa aaattgattt tagtagggga aaccccggac gagaaagcag 2821 tgcaatattt actgcaatgg ttggagaatc gggaagtgca agacgttttc ttcacctctt 2881 ccctctcaca aatttatcct gatgcagtga attttaaatc cgtggccagt ggcttattgg 2941 ccattcccat tgcccgtcac aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg 3001 ttaattgggg cggtgaccca aatcatgctt acgaagctac ccaggaagac ggtaaaatcg 3061 agctccatcc ccgccaatcc tttgacctct ggaaagaaat tgtccgactc caatctttgc 3121 cctggcaatc ggtggaaatc caaagtgccc tggccctgaa aaaggcgatc gtcaacctca 3181 ttttgcgcca ggcagaagaa ttgcatatgg cggctggtgt taagcaactg gcggatgacc 3241 gcacgctgct gatggcgggg gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc 3301 tggcgactga gatgatgagc gagcaggatg gctatctggc agaatcgatc aataaagata 3361 tcgaagagtg caacgccatc attgagcagt ttatcgacta cctgcgcacc gggcaggaga 3421 tgccgatgga aatggcggat cttaatgcag tactcggtga ggtgattgct gccgaaagtg 3481 gctatgagcg ggaaattgaa accgcgcttt accccggcag cattgaagtg aaaatgcacc 3541 cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg 3601 gctgggtcaa agtcagcagc ggaacggagc cgaatcgcgc ctggttccag gtggaagatg 3661 acggtccggg aattgcgccg gaacaacgta agcacctgtt ccagccgttt gtccgcggcg 3721 acagtgcgcg caccattagc ggcacgggat tagggctggc aattgtgcag cgtatcgtgg 3781 ataaccataa cgggatgctg gagcttggca ccagcgagcg gggcgggctt tccattcgcg 3841 cctggctgcc agtgccggta acgcgggcgc agggcatgac aaaagaaggg taataactct 3901 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc 3961 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 4021 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc ttgcatttac 4081 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg 4141 ttggattatt ctgcattttt ggggagaatg gacttactag agaaagagga gaaatactag 4201 atgtgtttaa agcaaatcat tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt 4261 cgtatcgaag aagatctgtt gggtaccagg gaagttccag ctgatgccta ctatggtgtt 4321 cacactctga gagcgattga aaacttctat atcagcaaca acaaaatcag tgatattcct 4381 gaatttgttc gcggtatggt aatggttaaa aaagccgcag ctatggcaaa caaagagctg 4441 caaaccattc ctaaaagtgt agcgaatgcc atcattgccg catgtgatga agtcctgaac 4501 aacggaaaat gcatggatca gttcccggta gacgtctacc agggcggcgc aggtacttcc 4561 gtaaacatga acaccaacga agtgctggcc aatatcggtc tggaactgat gggtcaccaa 4621 aaaggtgaat atcagtacct gaacccgaac gaccatgtta acaaatgtca gtccactaac 4681 gacgcctacc cgaccggttt ccgtatcgca gtttactctt ccctgattaa gctggtagat 4741 gcgattaacc aactgcgtga aggctttgaa cgtaaagctg tcgaatttca ggacatcctg 4801 aaaatgggtc gtacccagct gcaagacgca gtaccgatga ccctcggtca ggaatttcgc 4861 gctttcagca tcctgctgaa agaagaagtg aaaaacatcc aacgtaccgc tgaactgctg 4921 ctggaagtta accttggtgc aacagcaatc ggtactggtc tgaacacgcc gaaagagtac 4981 tctccgctgg cagtgaaaaa actggctgaa gttactggct tcccatgcgt accggctgaa 5041 gacctgatcg aagcgacctc tgactgcggc gcttatgtta tggttcacgg cgcgctgaaa 5101 cgcctggctg tgaagatgtc caaaatctgt aacgacctgc gcttgctctc ttcaggccca 5161 cgtgccggcc tgaacgagat caacctgccg gaactgcaag cgggctcttc catcatgcca 5221 gctaaagtaa acccggttgt tccggaagtg gttaaccagg tatgcttcaa agtcatcggt 5281 aacgacacca ctgttaccat ggcagcagaa gcaggtcagc tgcaattgaa cgttatggag 5341 ccggtcattg gccaggccat gttcgaatcc gttcacattc tgaccaacgc ttgctacaac 5401 ctgctggaaa aatgcattaa cggcatcact gctaacaaag aagtgtgcga aggttacgtt 5461 tacaactcta tcggtatcgt tacttacctg aacccgttca tcggtcacca caacggtgac 5521 atcgtgggta aaatctgtgc cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc 5581 ggtctgttga ctgaagcgga acttgacgat attttctccg tacagaatct gatgcacccg 5641 gcttacaaag caaaacgcta tactgatgaa agcgaacagg ctgcaaacga cgaaaactac 5701 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag 5761 aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag 5821 tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta 5881 gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga atatatttat 5941 gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat 6001 tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct 6061 aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct 6121 gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt 6181 attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa 6241 gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt 6301 gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac 6361 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 6421 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 6481 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 6541 gcgtttata //