##gff-version 3
##sequence-region BBa_J26100 1 6549
BBa_J26100	.	engineered_region	1	4174	.	+	0	ID=BBa_J26007;Name=BBa_J26007
BBa_J26100	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J26007
BBa_J26100	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J26100	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J26100	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J26100	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J26100	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J26100	.	promoter	4067	4174	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_J26007
BBa_J26100	.	promoter	4141	4146	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J26100	.	promoter	4164	4169	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J26100	.	non_covalent_binding_site	4120	4137	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J26100	.	non_covalent_binding_site	4079	4096	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J26100	.	non_covalent_binding_site	4100	4117	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J26100	.	engineered_region	1641	4058	.	+	0	ID=BBa_S03417;Name=BBa_S03417;Parent=BBa_J26007
BBa_J26100	.	ribosome_entry_site	1641	1652	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03417
BBa_J26100	.	conserved_region	1645	1648	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J26100	.	terminator	4018	4058	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03417
BBa_J26100	.	polyA_site	4045	4058	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J26100	.	engineered_region	4018	4058	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J26100	.	stem_loop	4025	4044	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J26100	.	stop_codon	4051	4051	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J26100	.	terminator	3930	4009	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03417
BBa_J26100	.	stem_loop	3941	3984	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J26100	.	engineered_region	3930	4009	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J26100	.	CDS	1659	3896	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_S03417
BBa_J26100	.	engineered_tag	3897	3921	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_J26100	.	CDS	1659	3896	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_J26100	.	engineered_region	63	831	.	+	0	ID=BBa_S03321;Name=BBa_S03321;Parent=BBa_J26007
BBa_J26100	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03321
BBa_J26100	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J26100	.	CDS	81	806	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_S03321
BBa_J26100	.	engineered_tag	807	831	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_J26100	.	engineered_region	840	1632	.	+	0	ID=BBa_S03322;Name=BBa_S03322;Parent=BBa_J26007
BBa_J26100	.	ribosome_entry_site	840	851	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03322
BBa_J26100	.	conserved_region	844	847	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J26100	.	CDS	858	1607	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_S03322
BBa_J26100	.	CDS	858	1607	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_J26100	.	engineered_tag	1608	1632	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_J26100	.	engineered_region	4183	6549	.	+	0	ID=BBa_J26008;Name=BBa_J26008
BBa_J26100	.	ribosome_entry_site	4183	4194	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J26008
BBa_J26100	.	conserved_region	4187	4190	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J26100	.	CDS	4201	5743	.	+	0	ID=BBa_C0083;Name=BBa_C0083;Parent=BBa_J26008
BBa_J26100	.	point_mutation	5325	5325	.	+	0	ID=annotation300988;Name=G;Parent=BBa_C0083
BBa_J26100	.	point_mutation	4788	4788	.	+	0	ID=annotation300983;Name=C;Parent=BBa_C0083
BBa_J26100	.	point_mutation	4201	4201	.	+	0	ID=annotation300982;Name=G;Parent=BBa_C0083
BBa_J26100	.	point_mutation	4824	4824	.	+	0	ID=annotation300984;Name=G;Parent=BBa_C0083
BBa_J26100	.	engineered_tag	5719	5743	.	+	0	ID=annotation2213998;Name=Help:Barcodes;Parent=BBa_C0083
BBa_J26100	.	CDS	4201	5679	.	+	0	ID=annotation300981;Name=AspA;Parent=BBa_C0083
BBa_J26100	.	tag	5680	5712	.	+	0	ID=annotation300993;Name=LVA;Parent=BBa_C0083
BBa_J26100	.	point_mutation	5199	5199	.	+	0	ID=annotation300987;Name=G;Parent=BBa_C0083
BBa_J26100	.	point_mutation	4857	4857	.	+	0	ID=annotation300986;Name=C;Parent=BBa_C0083
BBa_J26100	.	engineered_region	5752	6549	.	+	0	ID=BBa_F1610;Name=BBa_F1610;Parent=BBa_J26008
BBa_J26100	.	terminator	6509	6549	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_J26100	.	polyA_site	6536	6549	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J26100	.	engineered_region	6509	6549	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J26100	.	stem_loop	6516	6535	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J26100	.	stop_codon	6542	6542	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J26100	.	CDS	5770	6387	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_J26100	.	CDS	5770	6348	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J26100	.	engineered_region	5770	6387	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J26100	.	tag	6349	6380	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J26100	.	engineered_tag	6388	6412	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J26100	.	terminator	6421	6500	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_J26100	.	stem_loop	6432	6475	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J26100	.	engineered_region	6421	6500	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J26100	.	ribosome_entry_site	5752	5763	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_J26100	.	conserved_region	5756	5759	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J26100
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtc
aaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgcca
tggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaag
cctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctat
gtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatc
tttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatt
tgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccacc
gccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctga
tcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccac
ctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcact
gatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgcta
gttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaa
gttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaaggg
ttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggcc
ccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatc
attggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgt
ttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtc
accaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaaca
acaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaa
gtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactaga
tggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattca
gccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcatttta
gggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtc
gcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttt
tgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgccc
ttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatg
ttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtga
tgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaaccc
gcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcgg
ttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatct
gaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccac
catcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacattt
ccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgac
cacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggcc
atttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttgg
agaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgt
ggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggtt
aattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcct
ttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaa
aaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgac
cgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatga
gcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcga
ctacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaa
agtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaac
gcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaa
tcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtc
cgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacg
ggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgca
gggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatga
aacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaaga
ggagaaatactagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatc
gaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaact
tctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctat
ggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaac
ggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaag
tgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaa
caaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagat
gcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagc
tgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacat
ccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagag
tactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcga
cctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacga
cctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatc
atgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactg
ttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcgaatccgt
tcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtgtgcgaa
ggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcgtgggta
aaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaacttgacga
tattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggctgcaaac
gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata