LOCUS       BBa_J28000              2402 bp    DNA     linear       22-Nov-2024
DEFINITION  Operon 1 (a)
ACCESSION   BBa_J28000
VERSION     BBa_J28000.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=-10
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     promoter        24..29
                     /label=-10
     misc_feature    1..46
                     /label=BBa_R1052
     promoter        1..46
                     /label=BBa_R1052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             76..783
                     /label=cI lambda
     misc_feature    784..816
                     /label=LVA
     misc_feature    823..847
                     /label=Help:Barcodes
     CDS             73..822
                     /label=BBa_C0051
     misc_feature    860..863
                     /label=conserved
     RBS             856..867
                     /label=BBa_B0034
     misc_feature    1453..1484
                     /label=LVA
     CDS             874..1452
                     /label=luxI
     misc_feature    874..1491
                     /label=BBa_C0061
     misc_feature    1492..1516
                     /label=Help:Barcodes
     CDS             874..1491
                     /label=BBa_C0061
     misc_feature    55..1516
                     /label=BBa_S03145
     misc_feature    1529..1532
                     /label=conserved
     RBS             1525..1536
                     /label=BBa_B0034
     CDS             1543..2250
                     /label=mCherry
     misc_feature    1894..1894
                     /label=C->T (removing PstI site)
     misc_feature    2251..2259
                     /label=LVA
     misc_feature    1543..2265
                     /label=BBa_J06505
     stem_loop       2285..2328
                     /label=stem_loop
     misc_feature    2274..2353
                     /label=BBa_B0010
     terminator      2274..2353
                     /label=BBa_B0010
     stem_loop       2369..2388
                     /label=T7 TE
     misc_feature    2395..2395
                     /label=stop
     polyA_site      2389..2402
                     /label=polya
     misc_feature    2362..2402
                     /label=BBa_B0012
     terminator      2362..2402
                     /label=BBa_B0012
     misc_feature    1525..2402
                     /label=BBa_S03540
     misc_feature    55..2402
                     /label=BBa_S03551
ORIGIN
        1 ttgacaaaca agatacattg tatgatacta caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
      481 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
      541 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
      601 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
      661 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
      721 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt
      781 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt
      841 agatcgctac tagagaaaga ggagaaatac tagatgacta taatgataaa aaaatcggat
      901 tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt
      961 aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat
     1021 aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg
     1081 cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt
     1141 caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa
     1201 aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata
     1261 tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata
     1321 gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat
     1381 gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa
     1441 gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     1501 tgctagtgta gatctctact agagaaagag gagaaatact agatggtgag caagggcgag
     1561 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1621 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1681 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     1741 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1801 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1861 ggcggcgtgg tgaccgtgac ccaggactcc tccttgcagg acggcgagtt catctacaag
     1921 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1981 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     2041 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     2101 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     2161 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2221 tccaccggcg gcatggacga gctgtacaag ttagtagctt aataatacta gagccaggca
     2281 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2341 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2401 ta
//