LOCUS       BBa_J28001              2385 bp    DNA     linear       25-Nov-2024
DEFINITION  Operon 1 (b)
ACCESSION   BBa_J28001
VERSION     BBa_J28001.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=-10
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     promoter        24..29
                     /label=-10
     misc_feature    1..46
                     /label=BBa_R1052
     promoter        1..46
                     /label=BBa_R1052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             76..783
                     /label=cI lambda
     misc_feature    784..816
                     /label=LVA
     misc_feature    823..847
                     /label=Help:Barcodes
     CDS             73..822
                     /label=BBa_C0051
     misc_feature    860..863
                     /label=conserved
     RBS             856..867
                     /label=BBa_B0034
     misc_feature    1453..1484
                     /label=LVA
     CDS             874..1452
                     /label=luxI
     misc_feature    874..1491
                     /label=BBa_C0061
     misc_feature    1492..1516
                     /label=Help:Barcodes
     CDS             874..1491
                     /label=BBa_C0061
     misc_feature    55..1516
                     /label=BBa_S03145
     misc_feature    1529..1532
                     /label=conserved
     RBS             1525..1536
                     /label=BBa_B0034
     CDS             1543..2217
                     /label=mrfp1
     misc_feature    2224..2248
                     /label=Help:Barcodes
     CDS             1543..2223
                     /label=BBa_E1010
     stem_loop       2268..2311
                     /label=stem_loop
     misc_feature    2257..2336
                     /label=BBa_B0010
     terminator      2257..2336
                     /label=BBa_B0010
     stem_loop       2352..2371
                     /label=T7 TE
     misc_feature    2378..2378
                     /label=stop
     polyA_site      2372..2385
                     /label=polya
     misc_feature    2345..2385
                     /label=BBa_B0012
     terminator      2345..2385
                     /label=BBa_B0012
     misc_feature    1525..2385
                     /label=BBa_I13507
     misc_feature    55..2385
                     /label=BBa_S03552
ORIGIN
        1 ttgacaaaca agatacattg tatgatacta caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
      481 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
      541 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
      601 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
      661 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
      721 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt
      781 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt
      841 agatcgctac tagagaaaga ggagaaatac tagatgacta taatgataaa aaaatcggat
      901 tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt
      961 aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat
     1021 aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg
     1081 cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt
     1141 caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa
     1201 aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata
     1261 tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata
     1321 gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat
     1381 gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa
     1441 gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     1501 tgctagtgta gatctctact agagaaagag gagaaatact agatggcttc ctccgaagac
     1561 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
     1621 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
     1681 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
     1741 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
     1801 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
     1861 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
     1921 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
     1981 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
     2041 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
     2101 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
     2161 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
     2221 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
     2281 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2341 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//