LOCUS       BBa_J28002              2384 bp    DNA     linear       22-Nov-2024
DEFINITION  Operon 2 (a)
ACCESSION   BBa_J28002
VERSION     BBa_J28002.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..744
                     /label=cI 434
     misc_feature    706..744
                     /label=LVA
     misc_feature    76..744
                     /label=BBa_C0052
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             76..744
                     /label=BBa_C0052
     misc_feature    58..769
                     /label=BBa_S0104
     misc_feature    782..785
                     /label=conserved
     RBS             778..789
                     /label=BBa_B0034
     CDS             796..1398
                     /label=LasI
     misc_feature    1399..1431
                     /label=LVA
     misc_feature    1438..1462
                     /label=Help:Barcodes
     CDS             796..1437
                     /label=BBa_C0078
     misc_feature    778..1462
                     /label=BBa_S03154
     misc_feature    58..1462
                     /label=BBa_S03541
     misc_feature    1475..1478
                     /label=conserved
     RBS             1471..1482
                     /label=BBa_B0034
     misc_feature    1489..2247
                     /label=BBa_E0044
     stem_loop       2267..2310
                     /label=stem_loop
     misc_feature    2256..2335
                     /label=BBa_B0010
     terminator      2256..2335
                     /label=BBa_B0010
     stem_loop       2351..2370
                     /label=T7 TE
     misc_feature    2377..2377
                     /label=stop
     polyA_site      2371..2384
                     /label=polya
     misc_feature    2344..2384
                     /label=BBa_B0012
     terminator      2344..2384
                     /label=BBa_B0012
     misc_feature    1471..2384
                     /label=BBa_S03553
     misc_feature    58..2384
                     /label=BBa_S03557
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagaaa
       61 gaggagaaat actagatgag tatttcttcc agggtaaaaa gcaaaagaat ccagcttgga
      121 cttaaccagg ctgaacttgc tcaaaaggtg gggactaccc agcagtctat agagcagctc
      181 gaaaacggta aaactaagcg accacgcttt ttaccagaac ttgcgtcagc tcttggcgta
      241 agtgttgact ggctgctcaa tggcacctct gattcgaatg ttagatttgt tgggcacgtt
      301 gagcccaaag ggaaatatcc attgattagc atggttagag ctggttcgtg gtgtgaagct
      361 tgtgaaccct acgatatcaa ggacattgat gaatggtatg acagtgacgt taacttatta
      421 ggcaatggat tctggctgaa ggttgaaggt gattccatga cctcacctgt aggtcaaagc
      481 atccctgaag gtcatatggt gttagtagat actggacggg agccagtgaa tggaagcctt
      541 gttgtagcca aactgactga cgcgaacgaa gcaacattca agaaactggt catagatggc
      601 ggtcagaagt acctgaaagg cctgaatcct tcatggccta tgactcctat caacggaaac
      661 tgcaagatta tcggtgttgt cgtggaagcg agggtaaaat tcgtagctgc aaacgacgaa
      721 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagaaa
      781 gaggagaaat actagatgat cgttcagatc ggtcgtcgtg aagagttcga caaaaaactg
      841 ctgggtgaaa tgcacaaact gcgtgctcag gttttcaaag aacgtaaagg ttgggacgtt
      901 tccgttatcg acgaaatgga aatcgacggt tacgacgctc tgtccccgta ctacatgctg
      961 atccaggaag acaccccgga agctcaggtt ttcggttgct ggcgtatctt cgacaccacc
     1021 ggtccgtaca tgctgaaaaa caccttcccg gaactgctgc acggtaaaga agctccgtgc
     1081 tccccgcaca tctgggaact gtcccgtttc gctatcaact ccggtcagaa aggttccctg
     1141 ggtttctccg actgcaccct ggaagctatg cgtgctctgg ctcgttactc cttgcagaac
     1201 gacatccaga ccctggttac cgttaccacc gttggtgttg aaaaaatgat gatccgtgct
     1261 ggtctggacg tttcccgttt cggtccgcac ctgaaaatcg gtatcgaacg tgctgttgct
     1321 ctgcgtatcg aactgaacgc taaaacccag atcgctctgt acggtggtgt tctggttgaa
     1381 cagcgtctgg ctgtttccgc tgctaacgac gaaaactacg ctctggttgc ttaataactc
     1441 tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat gcgtaaagga
     1501 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1561 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1621 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1681 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1741 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1801 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1861 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1921 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     1981 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     2041 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2101 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2161 acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac
     2221 gaaaactacg ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc
     2281 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2341 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//