LOCUS       BBa_J28004              2444 bp    DNA     linear       17-May-2024
DEFINITION  Operon 2 (c)
ACCESSION   BBa_J28004
VERSION     BBa_J28004.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..744
                     /label=cI 434
     misc_feature    706..744
                     /label=LVA
     misc_feature    76..744
                     /label=BBa_C0052
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             76..744
                     /label=BBa_C0052
     misc_feature    58..769
                     /label=BBa_S0104
     misc_feature    782..785
                     /label=conserved
     RBS             778..789
                     /label=BBa_B0034
     CDS             796..1458
                     /label=CinI
     misc_feature    1459..1491
                     /label=LVA
     misc_feature    1498..1522
                     /label=Help:Barcodes
     CDS             796..1497
                     /label=BBa_C0076
     misc_feature    778..1522
                     /label=BBa_I9030
     misc_feature    58..1522
                     /label=BBa_S03543
     misc_feature    1535..1538
                     /label=conserved
     RBS             1531..1542
                     /label=BBa_B0034
     misc_feature    1549..2307
                     /label=BBa_E0044
     stem_loop       2327..2370
                     /label=stem_loop
     misc_feature    2316..2395
                     /label=BBa_B0010
     terminator      2316..2395
                     /label=BBa_B0010
     stem_loop       2411..2430
                     /label=T7 TE
     misc_feature    2437..2437
                     /label=stop
     polyA_site      2431..2444
                     /label=polya
     misc_feature    2404..2444
                     /label=BBa_B0012
     terminator      2404..2444
                     /label=BBa_B0012
     misc_feature    1531..2444
                     /label=BBa_S03553
     misc_feature    58..2444
                     /label=BBa_S03559
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagaaa
       61 gaggagaaat actagatgag tatttcttcc agggtaaaaa gcaaaagaat ccagcttgga
      121 cttaaccagg ctgaacttgc tcaaaaggtg gggactaccc agcagtctat agagcagctc
      181 gaaaacggta aaactaagcg accacgcttt ttaccagaac ttgcgtcagc tcttggcgta
      241 agtgttgact ggctgctcaa tggcacctct gattcgaatg ttagatttgt tgggcacgtt
      301 gagcccaaag ggaaatatcc attgattagc atggttagag ctggttcgtg gtgtgaagct
      361 tgtgaaccct acgatatcaa ggacattgat gaatggtatg acagtgacgt taacttatta
      421 ggcaatggat tctggctgaa ggttgaaggt gattccatga cctcacctgt aggtcaaagc
      481 atccctgaag gtcatatggt gttagtagat actggacggg agccagtgaa tggaagcctt
      541 gttgtagcca aactgactga cgcgaacgaa gcaacattca agaaactggt catagatggc
      601 ggtcagaagt acctgaaagg cctgaatcct tcatggccta tgactcctat caacggaaac
      661 tgcaagatta tcggtgttgt cgtggaagcg agggtaaaat tcgtagctgc aaacgacgaa
      721 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagaaa
      781 gaggagaaat actagatgtt cgttatcatc caggctcacg aataccagaa atacgctgct
      841 gttctggacc agatgttccg tctgcgtaaa aaagttttcg ctgacaccct gtgctgggac
      901 gttccggtta tcggtccgta cgaacgtgac tcctacgact ccctggctcc ggcttacctg
      961 gtttggtgca acgactcccg tacccgtctg tacggtggta tgcgtctgat gccgaccacc
     1021 ggtccgaccc tgctgtacga cgttttccgt gaaaccttcc cggacgctgc tgacctgatc
     1081 gctccgggta tctgggaagg tacccgtatg tgcatcgacg aagaagctat cgctaaagac
     1141 ttcccggaaa tcgacgctgg tcgtgctttc tccatgatgc tgctggctct gtgcgaatgc
     1201 gctctggacc acggtatcca caccatgatc tccaactacg aaccgtacct gaaacgtgtt
     1261 tacaaacgtg ctggtgctga agttgaagaa ctgggtcgtg ctgacggtta cggtaaatac
     1321 ccggtttgct gcggtgcttt cgaagtttcc gaccgtgttc tgcgtaaaat gcgtgctgct
     1381 ctgggtctga ccctgccgct gtacgttcgt cacgttccgg ctcgttccgt tgttacccag
     1441 ttcctggaaa tggctgctgc tgctaacgac gaaaactacg ctctggttgc ttaataaccc
     1501 tgatagtgct agtgtagatc cctactagag aaagaggaga aatactagat gcgtaaagga
     1561 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1621 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1681 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1741 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1801 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1861 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1921 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1981 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     2041 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     2101 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2161 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2221 acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac
     2281 gaaaactacg ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc
     2341 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2401 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//