LOCUS       BBa_J28005              2642 bp    DNA     linear       22-Dec-2024
DEFINITION  Operon 3 (a)
ACCESSION   BBa_J28005
VERSION     BBa_J28005.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     CDS             184..852
                     /label=cI 434
     misc_feature    814..852
                     /label=LVA
     misc_feature    184..852
                     /label=BBa_C0052
     misc_feature    853..877
                     /label=Help:Barcodes
     CDS             184..852
                     /label=BBa_C0052
     misc_feature    166..877
                     /label=BBa_S0104
     misc_feature    890..893
                     /label=conserved
     RBS             886..897
                     /label=BBa_B0034
     misc_feature    904..906
                     /label=start
     misc_feature    1687..1692
                     /label=2
     misc_feature    1654..1686
                     /label=LVA
     CDS             904..1653
                     /label=aiiA
     misc_feature    904..1692
                     /label=BBa_C0060
     misc_feature    1693..1717
                     /label=Help:Barcodes
     CDS             904..1692
                     /label=BBa_C0060
     misc_feature    886..1717
                     /label=BBa_C0260
     misc_feature    1730..1733
                     /label=conserved
     RBS             1726..1737
                     /label=BBa_B0034
     CDS             1744..2505
                     /label=YFP (LVA)
     misc_feature    2500..2505
                     /label=2
     misc_feature    2461..2499
                     /label=SsrA
     misc_feature    1985..1985
                     /label=A (Q->L)
     misc_feature    1744..2505
                     /label=BBa_E0032
     misc_feature    1744..2505
                     /label=BBa_E0032
     stem_loop       2525..2568
                     /label=stem_loop
     misc_feature    2514..2593
                     /label=BBa_B0010
     terminator      2514..2593
                     /label=BBa_B0010
     stem_loop       2609..2628
                     /label=T7 TE
     misc_feature    2635..2635
                     /label=stop
     polyA_site      2629..2642
                     /label=polya
     misc_feature    2602..2642
                     /label=BBa_B0012
     terminator      2602..2642
                     /label=BBa_B0012
     misc_feature    1726..2642
                     /label=BBa_E0432
     misc_feature    886..2642
                     /label=BBa_S03545
     misc_feature    166..2642
                     /label=BBa_S03554
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatgagta tttcttccag ggtaaaaagc aaaagaatcc agcttggact taaccaggct
      241 gaacttgctc aaaaggtggg gactacccag cagtctatag agcagctcga aaacggtaaa
      301 actaagcgac cacgcttttt accagaactt gcgtcagctc ttggcgtaag tgttgactgg
      361 ctgctcaatg gcacctctga ttcgaatgtt agatttgttg ggcacgttga gcccaaaggg
      421 aaatatccat tgattagcat ggttagagct ggttcgtggt gtgaagcttg tgaaccctac
      481 gatatcaagg acattgatga atggtatgac agtgacgtta acttattagg caatggattc
      541 tggctgaagg ttgaaggtga ttccatgacc tcacctgtag gtcaaagcat ccctgaaggt
      601 catatggtgt tagtagatac tggacgggag ccagtgaatg gaagccttgt tgtagccaaa
      661 ctgactgacg cgaacgaagc aacattcaag aaactggtca tagatggcgg tcagaagtac
      721 ctgaaaggcc tgaatccttc atggcctatg actcctatca acggaaactg caagattatc
      781 ggtgttgtcg tggaagcgag ggtaaaattc gtagctgcaa acgacgaaaa ctacgcttta
      841 gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac
      901 tagatgacag taaagaagct ttatttcgtc ccagcaggtc gttgtatgtt ggatcattcg
      961 tctgttaata gtacattaac accaggagaa ttattagact taccggtttg gtgttatctt
     1021 ttggagactg aagaaggacc tattttagta gatacaggta tgccagaaag tgcagttaat
     1081 aatgaaggtc tttttaacgg tacatttgtc gaagggcagg ttttaccgaa aatgactgaa
     1141 gaagatagaa tcgtgaatat tttaaaacgg gttggttatg agccggaaga ccttctttat
     1201 attattagtt ctcacttgca ttttgatcat gcaggaggaa atggcgcttt tataaataca
     1261 ccaatcattg tacagcgtgc tgaatatgag gcggcgcagc atagcgaaga atatttgaaa
     1321 gaatgtatat tgccgaattt aaactacaaa atcattgaag gtgattatga agtcgtacca
     1381 ggagttcaat tattgcatac accaggccat actccagggc atcaatcgct attaattgag
     1441 acagaaaaat ccggtcctgt attattaacg attgatgcat cgtatacgaa agagaatttt
     1501 gaaaatgaag tgccatttgc gggatttgat tcagaattag ctttatcttc aattaaacgt
     1561 ttaaaagaag tggtgatgaa agagaagccg attgttttct ttggacatga tatagagcag
     1621 gaaaggggat gtaaagtgtt ccctgaatat atagctgcaa acgacgaaaa ctacgcttta
     1681 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagaaaga ggagaaatac
     1741 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1801 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1861 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1921 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
     1981 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2041 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2101 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2161 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2221 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     2281 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2341 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2401 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2461 aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta gagccaggca
     2521 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2581 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2641 ta
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