##gff-version 3
##sequence-region BBa_J28006 1 2538
BBa_J28006	.	promoter	1	53	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_J28006	.	promoter	42	47	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_J28006	.	start_codon	53	53	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_J28006	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_J28006	.	promoter	19	24	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_J28006	.	engineered_region	62	2538	.	+	0	ID=BBa_S03554;Name=BBa_S03554
BBa_J28006	.	engineered_region	782	2538	.	+	0	ID=BBa_S03545;Name=BBa_S03545;Parent=BBa_S03554
BBa_J28006	.	engineered_region	1622	2538	.	+	0	ID=BBa_E0432;Name=BBa_E0432;Parent=BBa_S03545
BBa_J28006	.	terminator	2410	2489	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_J28006	.	stem_loop	2421	2464	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28006	.	engineered_region	2410	2489	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28006	.	ribosome_entry_site	1622	1633	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_J28006	.	conserved_region	1626	1629	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28006	.	engineered_region	1640	2401	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_J28006	.	CDS	1640	2401	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J28006	.	tag	2357	2395	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J28006	.	engineered_region	1640	2401	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J28006	.	stop_codon	2396	2401	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J28006	.	sequence_alteration	1881	1881	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J28006	.	terminator	2498	2538	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_J28006	.	engineered_region	2498	2538	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28006	.	stop_codon	2531	2531	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28006	.	stem_loop	2505	2524	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28006	.	polyA_site	2525	2538	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28006	.	engineered_region	782	1613	.	+	0	ID=BBa_C0260;Name=BBa_C0260;Parent=BBa_S03545
BBa_J28006	.	ribosome_entry_site	782	793	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0260
BBa_J28006	.	conserved_region	786	789	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28006	.	CDS	800	1588	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_C0260
BBa_J28006	.	stop_codon	1583	1588	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_J28006	.	start_codon	800	802	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_J28006	.	engineered_region	800	1588	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_J28006	.	engineered_tag	1589	1613	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_J28006	.	tag	1550	1582	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_J28006	.	CDS	800	1549	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_J28006	.	engineered_region	62	773	.	+	0	ID=BBa_S0104;Name=BBa_S0104;Parent=BBa_S03554
BBa_J28006	.	ribosome_entry_site	62	73	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0104
BBa_J28006	.	conserved_region	66	69	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28006	.	CDS	80	748	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_S0104
BBa_J28006	.	CDS	80	748	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_J28006	.	tag	710	748	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_J28006	.	engineered_region	80	748	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_J28006	.	engineered_tag	749	773	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
>BBa_J28006
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactag
atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtgggga
ctacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctct
tggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaaggg
aaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatg
aatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgt
aggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagcc
aaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatc
cttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagc
tgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggag
aaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagta
cattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtaga
tacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaa
atgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagtt
ctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatga
ggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattat
gaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaa
aatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatt
tgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttgga
catgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtag
cttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggtgagcaagggcgagga
gctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgag
ggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccca
ccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaa
gtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgag
gtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgg
ggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaa
cttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgac
ggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatc
acatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaa
cgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata