LOCUS       BBa_J28008              2621 bp    DNA     linear       26-Dec-2024
DEFINITION  Operon 4 (a)
ACCESSION   BBa_J28008
VERSION     BBa_J28008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..831
                     /label=BBa_C0051
     misc_feature    64..856
                     /label=BBa_J06550
     misc_feature    869..872
                     /label=conserved
     RBS             865..876
                     /label=BBa_B0034
     misc_feature    883..885
                     /label=start
     misc_feature    1666..1671
                     /label=2
     misc_feature    1633..1665
                     /label=LVA
     CDS             883..1632
                     /label=aiiA
     misc_feature    883..1671
                     /label=BBa_C0060
     misc_feature    1672..1696
                     /label=Help:Barcodes
     CDS             883..1671
                     /label=BBa_C0060
     misc_feature    865..1696
                     /label=BBa_C0260
     misc_feature    1709..1712
                     /label=conserved
     RBS             1705..1716
                     /label=BBa_B0034
     CDS             1723..2484
                     /label=CFP (LVA)
     misc_feature    2441..2478
                     /label=SsrA
     misc_feature    2479..2484
                     /label=2
     misc_feature    1791..1791
                     /label=A
     misc_feature    1723..2484
                     /label=BBa_E0022
     misc_feature    1723..2484
                     /label=BBa_E0022
     stem_loop       2504..2547
                     /label=stem_loop
     misc_feature    2493..2572
                     /label=BBa_B0010
     terminator      2493..2572
                     /label=BBa_B0010
     stem_loop       2588..2607
                     /label=T7 TE
     misc_feature    2614..2614
                     /label=stop
     polyA_site      2608..2621
                     /label=polya
     misc_feature    2581..2621
                     /label=BBa_B0012
     terminator      2581..2621
                     /label=BBa_B0012
     misc_feature    1705..2621
                     /label=BBa_E0422
     misc_feature    865..2621
                     /label=BBa_S03546
     misc_feature    64..2621
                     /label=BBa_S03555
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagaaagag gagaaatact agatgacagt aaagaagctt
      901 tatttcgtcc cagcaggtcg ttgtatgttg gatcattcgt ctgttaatag tacattaaca
      961 ccaggagaat tattagactt accggtttgg tgttatcttt tggagactga agaaggacct
     1021 attttagtag atacaggtat gccagaaagt gcagttaata atgaaggtct ttttaacggt
     1081 acatttgtcg aagggcaggt tttaccgaaa atgactgaag aagatagaat cgtgaatatt
     1141 ttaaaacggg ttggttatga gccggaagac cttctttata ttattagttc tcacttgcat
     1201 tttgatcatg caggaggaaa tggcgctttt ataaatacac caatcattgt acagcgtgct
     1261 gaatatgagg cggcgcagca tagcgaagaa tatttgaaag aatgtatatt gccgaattta
     1321 aactacaaaa tcattgaagg tgattatgaa gtcgtaccag gagttcaatt attgcataca
     1381 ccaggccata ctccagggca tcaatcgcta ttaattgaga cagaaaaatc cggtcctgta
     1441 ttattaacga ttgatgcatc gtatacgaaa gagaattttg aaaatgaagt gccatttgcg
     1501 ggatttgatt cagaattagc tttatcttca attaaacgtt taaaagaagt ggtgatgaaa
     1561 gagaagccga ttgttttctt tggacatgat atagagcagg aaaggggatg taaagtgttc
     1621 cctgaatata tagctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
     1681 tgctagtgta gatcgctact agagaaagag gagaaatact agatggtgag caagggcgag
     1741 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac
     1801 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1861 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1921 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1981 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2041 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2101 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2161 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     2221 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2281 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2341 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2401 gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctgctgc aaacgacgaa
     2461 aactacgctt tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca
     2521 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2581 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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