BBa_J28012
type: ComponentDefinition
persistent identity: BBa J28012
display id: BBa_J28012
version: 1
was generated by: iGEM to SBOL conversion
description: Operon 6 (a)
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: soon
owned by: james
owned by: myers
mutable description: soon
discontinued: false
dominant: false
group u list: _57_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: soon
star: false
creator: Kaj Bernhardt
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I0462
component: BBa_S03549
sequence annotation: BBa_S03549
sequence annotation: BBa_I0462
sequence: https://synbiohub.org/public/igem/BBa_J28012_sequence/1

BBa_I0462
type: Component
persistent identity: component2260170
display id: component2260170
version: 1
top level: BBa_J28012
definition: LuxR
access: public

BBa_S03549
type: Component
persistent identity: component2260155
display id: component2260155
version: 1
top level: BBa_J28012
definition: BBa_S03549
access: public

BBa_S03549
type: SequenceAnnotation
persistent identity: annotation2260155
display id: annotation2260155
version: 1
top level: BBa_J28012
location: https://synbiohub.org/public/igem/BBa_J28012/annotation2260155/range2260155/1
component: BBa_S03549

https://synbiohub.org/public/igem/BBa_J28012/annotation2260155/range2260155/1
type: Range
persistent identity: range2260155
display id: range2260155
version: 1
top level: BBa_J28012
direction: direction/reverse
start: 1
end: 340
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2260170
display id: annotation2260170
version: 1
top level: BBa_J28012
location: https://synbiohub.org/public/igem/BBa_J28012/annotation2260170/range2260170/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_J28012/annotation2260170/range2260170/1
type: Range
persistent identity: range2260170
display id: range2260170
version: 1
top level: BBa_J28012
direction: direction/reverse
start: 349
end: 1284
orientation: inline

BBa_S03549
type: ComponentDefinition
persistent identity: BBa S03549
display id: BBa_S03549
version: 1
was generated by: iGEM to SBOL conversion
description: R0081:R0080
created: 2006-08-13T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _57_
m user id: 0
owner id: 1072
owning group id: 57
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Kaj Bernhardt
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_R0080
component: BBa_R0081
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence: https://synbiohub.org/public/igem/BBa_S03549_sequence/1

BBa_R0080
type: Component
persistent identity: component1895854
display id: component1895854
version: 1
top level: BBa_S03549
definition: AraC
access: public

BBa_R0081
type: Component
persistent identity: component1895847
display id: component1895847
version: 1
top level: BBa_S03549
definition: AraC O2
access: public

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation1895847
display id: annotation1895847
version: 1
top level: BBa_S03549
location: https://synbiohub.org/public/igem/BBa_S03549/annotation1895847/range1895847/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_S03549/annotation1895847/range1895847/1
type: Range
persistent identity: range1895847
display id: range1895847
version: 1
top level: BBa_S03549
direction: direction/reverse
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation1895854
display id: annotation1895854
version: 1
top level: BBa_S03549
location: https://synbiohub.org/public/igem/BBa_S03549/annotation1895854/range1895854/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_S03549/annotation1895854/range1895854/1
type: Range
persistent identity: range1895854
display id: range1895854
version: 1
top level: BBa_S03549
direction: direction/reverse
start: 192
end: 340
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (AraC regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
top level: AraC
direction: direction/forward
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
top level: AraC
direction: direction/forward
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
top level: AraC
direction: direction/forward
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
top level: AraC
direction: direction/forward
start: 73
end: 101
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
top level: AraC
direction: direction/forward
start: 113
end: 118
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
top level: AraC
direction: direction/forward
start: 136
end: 141
orientation: inline

AraC O2
type: ComponentDefinition
persistent identity: BBa R0081
display id: BBa_R0081
version: 1
was generated by: iGEM to SBOL conversion
description: Inhibitor (AraC loop attachment with O2 site)
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: this piece of DNA contains a "dead" part of coding region for AraC, and a functional Operator2 site. when attached to the rest of the promoter region, this part allows for the binding and loop formation that would result in repression of the araC promoter
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 1) changed ATG start codon to TAG stop 2) 6 random deletions upstream of putative araC binding sites to preserve spacing for loop back (BB ends will add 6 base pairs); spacing crucial in matching up O2 and I2 binding sites (turns of DNA helix) 3) designed to attach to R0080 to enable all (positive and negative) regulatory functions, and to form "loop back"
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: defunct araC
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0081_sequence/1

defunct araC
type: SequenceAnnotation
persistent identity: annotation331785
display id: annotation331785
version: 1
top level: AraC O2
location: https://synbiohub.org/public/igem/BBa_R0081/annotation331785/range331785/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0081/annotation331785/range331785/1
type: Range
persistent identity: range331785
display id: range331785
version: 1
top level: AraC O2
direction: direction/forward
start: 1
end: 78
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation331786
display id: annotation331786
version: 1
top level: AraC O2
location: https://synbiohub.org/public/igem/BBa_R0081/annotation331786/range331786/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0081/annotation331786/range331786/1
type: Range
persistent identity: range331786
display id: range331786
version: 1
top level: AraC O2
direction: direction/forward
start: 22
end: 37
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation331787
display id: annotation331787
version: 1
top level: AraC O2
location: https://synbiohub.org/public/igem/BBa_R0081/annotation331787/range331787/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0081/annotation331787/range331787/1
type: Range
persistent identity: range331787
display id: range331787
version: 1
top level: AraC O2
direction: direction/forward
start: 57
end: 72
orientation: inline

https://synbiohub.org/public/igem/BBa_J28012_sequence/1
type: Sequence
persistent identity: BBa J28012 sequence
display id: BBa_J28012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgctacagacattgccgtcactggtctttactggctcttctcgctaaccaaaccggtaaccgcttattaaagcattctgtaacaaagcgggaccaaagccatgacaaaactactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03549_sequence/1
type: Sequence
persistent identity: BBa S03549 sequence
display id: BBa_S03549_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgctacagacattgccgtcactggtctttactggctcttctcgctaaccaaaccggtaaccgcttattaaagcattctgtaacaaagcgggaccaaagccatgacaaaactactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0080_sequence/1
type: Sequence
persistent identity: BBa R0080 sequence
display id: BBa_R0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0081_sequence/1
type: Sequence
persistent identity: BBa R0081 sequence
display id: BBa_R0081_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgctacagacattgccgtcactggtctttactggctcttctcgctaaccaaaccggtaaccgcttattaaagcattctgtaacaaagcgggaccaaagccatgacaaaac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z