##gff-version 3
##sequence-region BBa_J28016 1 1353
BBa_J28016	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_J28016	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J28016	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J28016	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J28016	.	engineered_region	46	1353	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_J28016	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_J28016	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28016	.	CDS	64	1191	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_J28016	.	engineered_region	64	1191	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J28016	.	engineered_tag	1192	1216	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J28016	.	CDS	64	1191	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J28016	.	tag	1153	1191	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J28016	.	terminator	1225	1304	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_J28016	.	stem_loop	1236	1279	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28016	.	engineered_region	1225	1304	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28016	.	terminator	1313	1353	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_J28016	.	stop_codon	1346	1346	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28016	.	engineered_region	1313	1353	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28016	.	stem_loop	1320	1339	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28016	.	polyA_site	1340	1353	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J28016
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatggtgaatgtgaaac
cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcca
cgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaa
ctggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcgg
cgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaa
agcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccatt
gctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattt
tctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcggg
cccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgata
gcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccca
ctgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgc
ggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggat
tttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgt
tgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagct
taataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata