LOCUS BBa_J28018 2642 bp DNA linear 23-Dec-2024 DEFINITION Operon 3 (d) ACCESSION BBa_J28018 VERSION BBa_J28018.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 CDS 184..852 /label=cI 434 misc_feature 814..852 /label=LVA misc_feature 184..852 /label=BBa_C0052 misc_feature 853..877 /label=Help:Barcodes CDS 184..852 /label=BBa_C0052 misc_feature 166..877 /label=BBa_S0104 misc_feature 890..893 /label=conserved RBS 886..897 /label=BBa_B0034 misc_feature 904..906 /label=start misc_feature 1687..1692 /label=2 misc_feature 1654..1686 /label=LVA CDS 904..1653 /label=aiiA misc_feature 904..1692 /label=BBa_C0060 misc_feature 1693..1717 /label=Help:Barcodes CDS 904..1692 /label=BBa_C0060 misc_feature 886..1717 /label=BBa_C0260 misc_feature 1730..1733 /label=conserved RBS 1726..1737 /label=BBa_B0034 misc_feature 2500..2505 /label=stop misc_feature 2461..2499 /label=AAV misc_feature 1744..2505 /label=BBa_E0034 stem_loop 2525..2568 /label=stem_loop misc_feature 2514..2593 /label=BBa_B0010 terminator 2514..2593 /label=BBa_B0010 stem_loop 2609..2628 /label=T7 TE misc_feature 2635..2635 /label=stop polyA_site 2629..2642 /label=polya misc_feature 2602..2642 /label=BBa_B0012 terminator 2602..2642 /label=BBa_B0012 misc_feature 1726..2642 /label=BBa_E0434 misc_feature 886..2642 /label=BBa_S03586 misc_feature 166..2642 /label=BBa_S03589 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatgagta tttcttccag ggtaaaaagc aaaagaatcc agcttggact taaccaggct 241 gaacttgctc aaaaggtggg gactacccag cagtctatag agcagctcga aaacggtaaa 301 actaagcgac cacgcttttt accagaactt gcgtcagctc ttggcgtaag tgttgactgg 361 ctgctcaatg gcacctctga ttcgaatgtt agatttgttg ggcacgttga gcccaaaggg 421 aaatatccat tgattagcat ggttagagct ggttcgtggt gtgaagcttg tgaaccctac 481 gatatcaagg acattgatga atggtatgac agtgacgtta acttattagg caatggattc 541 tggctgaagg ttgaaggtga ttccatgacc tcacctgtag gtcaaagcat ccctgaaggt 601 catatggtgt tagtagatac tggacgggag ccagtgaatg gaagccttgt tgtagccaaa 661 ctgactgacg cgaacgaagc aacattcaag aaactggtca tagatggcgg tcagaagtac 721 ctgaaaggcc tgaatccttc atggcctatg actcctatca acggaaactg caagattatc 781 ggtgttgtcg tggaagcgag ggtaaaattc gtagctgcaa acgacgaaaa ctacgcttta 841 gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac 901 tagatgacag taaagaagct ttatttcgtc ccagcaggtc gttgtatgtt ggatcattcg 961 tctgttaata gtacattaac accaggagaa ttattagact taccggtttg gtgttatctt 1021 ttggagactg aagaaggacc tattttagta gatacaggta tgccagaaag tgcagttaat 1081 aatgaaggtc tttttaacgg tacatttgtc gaagggcagg ttttaccgaa aatgactgaa 1141 gaagatagaa tcgtgaatat tttaaaacgg gttggttatg agccggaaga ccttctttat 1201 attattagtt ctcacttgca ttttgatcat gcaggaggaa atggcgcttt tataaataca 1261 ccaatcattg tacagcgtgc tgaatatgag gcggcgcagc atagcgaaga atatttgaaa 1321 gaatgtatat tgccgaattt aaactacaaa atcattgaag gtgattatga agtcgtacca 1381 ggagttcaat tattgcatac accaggccat actccagggc atcaatcgct attaattgag 1441 acagaaaaat ccggtcctgt attattaacg attgatgcat cgtatacgaa agagaatttt 1501 gaaaatgaag tgccatttgc gggatttgat tcagaattag ctttatcttc aattaaacgt 1561 ttaaaagaag tggtgatgaa agagaagccg attgttttct ttggacatga tatagagcag 1621 gaaaggggat gtaaagtgtt ccctgaatat atagctgcaa acgacgaaaa ctacgcttta 1681 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagaaaga ggagaaatac 1741 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 1801 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 1861 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 1921 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg 1981 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 2041 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 2101 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 2161 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 2221 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 2281 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 2341 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 2401 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 2461 aggcctgctg caaacgacga aaactacgct gctgctgttt aataatacta gagccaggca 2521 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2581 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 2641 ta //