LOCUS BBa_J28019 2538 bp DNA linear 22-Nov-2024 DEFINITION Operon 3 (e) ACCESSION BBa_J28019 VERSION BBa_J28019.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=RhlR promoter 19..24 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start promoter 1..53 /label=BBa_R0071 misc_feature 66..69 /label=conserved RBS 62..73 /label=BBa_B0034 CDS 80..748 /label=cI 434 misc_feature 710..748 /label=LVA misc_feature 80..748 /label=BBa_C0052 misc_feature 749..773 /label=Help:Barcodes CDS 80..748 /label=BBa_C0052 misc_feature 62..773 /label=BBa_S0104 misc_feature 786..789 /label=conserved RBS 782..793 /label=BBa_B0034 misc_feature 800..802 /label=start misc_feature 1583..1588 /label=2 misc_feature 1550..1582 /label=LVA CDS 800..1549 /label=aiiA misc_feature 800..1588 /label=BBa_C0060 misc_feature 1589..1613 /label=Help:Barcodes CDS 800..1588 /label=BBa_C0060 misc_feature 782..1613 /label=BBa_C0260 misc_feature 1626..1629 /label=conserved RBS 1622..1633 /label=BBa_B0034 misc_feature 2396..2401 /label=stop misc_feature 2357..2395 /label=AAV misc_feature 1640..2401 /label=BBa_E0034 stem_loop 2421..2464 /label=stem_loop misc_feature 2410..2489 /label=BBa_B0010 terminator 2410..2489 /label=BBa_B0010 stem_loop 2505..2524 /label=T7 TE misc_feature 2531..2531 /label=stop polyA_site 2525..2538 /label=polya misc_feature 2498..2538 /label=BBa_B0012 terminator 2498..2538 /label=BBa_B0012 misc_feature 1622..2538 /label=BBa_E0434 misc_feature 782..2538 /label=BBa_S03586 misc_feature 62..2538 /label=BBa_S03589 ORIGIN 1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga 61 gaaagaggag aaatactaga tgagtatttc ttccagggta aaaagcaaaa gaatccagct 121 tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt ctatagagca 181 gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt cagctcttgg 241 cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat ttgttgggca 301 cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt cgtggtgtga 361 agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg acgttaactt 421 attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac ctgtaggtca 481 aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag tgaatggaag 541 ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac tggtcataga 601 tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc ctatcaacgg 661 aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtag ctgcaaacga 721 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 781 gaaagaggag aaatactaga tgacagtaaa gaagctttat ttcgtcccag caggtcgttg 841 tatgttggat cattcgtctg ttaatagtac attaacacca ggagaattat tagacttacc 901 ggtttggtgt tatcttttgg agactgaaga aggacctatt ttagtagata caggtatgcc 961 agaaagtgca gttaataatg aaggtctttt taacggtaca tttgtcgaag ggcaggtttt 1021 accgaaaatg actgaagaag atagaatcgt gaatatttta aaacgggttg gttatgagcc 1081 ggaagacctt ctttatatta ttagttctca cttgcatttt gatcatgcag gaggaaatgg 1141 cgcttttata aatacaccaa tcattgtaca gcgtgctgaa tatgaggcgg cgcagcatag 1201 cgaagaatat ttgaaagaat gtatattgcc gaatttaaac tacaaaatca ttgaaggtga 1261 ttatgaagtc gtaccaggag ttcaattatt gcatacacca ggccatactc cagggcatca 1321 atcgctatta attgagacag aaaaatccgg tcctgtatta ttaacgattg atgcatcgta 1381 tacgaaagag aattttgaaa atgaagtgcc atttgcggga tttgattcag aattagcttt 1441 atcttcaatt aaacgtttaa aagaagtggt gatgaaagag aagccgattg ttttctttgg 1501 acatgatata gagcaggaaa ggggatgtaa agtgttccct gaatatatag ctgcaaacga 1561 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga 1621 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 1681 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 1741 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 1801 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg 1861 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt 1921 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 1981 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 2041 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 2101 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 2161 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 2221 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 2281 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 2341 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctgctg ctgtttaata 2401 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2461 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2521 ggcctttctg cgtttata //