LOCUS       BBa_J28019              2538 bp    DNA     linear       23-May-2024
DEFINITION  Operon 3 (e)
ACCESSION   BBa_J28019
VERSION     BBa_J28019.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     misc_feature    66..69
                     /label=conserved
     RBS             62..73
                     /label=BBa_B0034
     CDS             80..748
                     /label=cI 434
     misc_feature    710..748
                     /label=LVA
     misc_feature    80..748
                     /label=BBa_C0052
     misc_feature    749..773
                     /label=Help:Barcodes
     CDS             80..748
                     /label=BBa_C0052
     misc_feature    62..773
                     /label=BBa_S0104
     misc_feature    786..789
                     /label=conserved
     RBS             782..793
                     /label=BBa_B0034
     misc_feature    800..802
                     /label=start
     misc_feature    1583..1588
                     /label=2
     misc_feature    1550..1582
                     /label=LVA
     CDS             800..1549
                     /label=aiiA
     misc_feature    800..1588
                     /label=BBa_C0060
     misc_feature    1589..1613
                     /label=Help:Barcodes
     CDS             800..1588
                     /label=BBa_C0060
     misc_feature    782..1613
                     /label=BBa_C0260
     misc_feature    1626..1629
                     /label=conserved
     RBS             1622..1633
                     /label=BBa_B0034
     misc_feature    2396..2401
                     /label=stop
     misc_feature    2357..2395
                     /label=AAV
     misc_feature    1640..2401
                     /label=BBa_E0034
     stem_loop       2421..2464
                     /label=stem_loop
     misc_feature    2410..2489
                     /label=BBa_B0010
     terminator      2410..2489
                     /label=BBa_B0010
     stem_loop       2505..2524
                     /label=T7 TE
     misc_feature    2531..2531
                     /label=stop
     polyA_site      2525..2538
                     /label=polya
     misc_feature    2498..2538
                     /label=BBa_B0012
     terminator      2498..2538
                     /label=BBa_B0012
     misc_feature    1622..2538
                     /label=BBa_E0434
     misc_feature    782..2538
                     /label=BBa_S03586
     misc_feature    62..2538
                     /label=BBa_S03589
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gaaagaggag aaatactaga tgagtatttc ttccagggta aaaagcaaaa gaatccagct
      121 tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt ctatagagca
      181 gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt cagctcttgg
      241 cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat ttgttgggca
      301 cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt cgtggtgtga
      361 agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg acgttaactt
      421 attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac ctgtaggtca
      481 aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag tgaatggaag
      541 ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac tggtcataga
      601 tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc ctatcaacgg
      661 aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtag ctgcaaacga
      721 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga
      781 gaaagaggag aaatactaga tgacagtaaa gaagctttat ttcgtcccag caggtcgttg
      841 tatgttggat cattcgtctg ttaatagtac attaacacca ggagaattat tagacttacc
      901 ggtttggtgt tatcttttgg agactgaaga aggacctatt ttagtagata caggtatgcc
      961 agaaagtgca gttaataatg aaggtctttt taacggtaca tttgtcgaag ggcaggtttt
     1021 accgaaaatg actgaagaag atagaatcgt gaatatttta aaacgggttg gttatgagcc
     1081 ggaagacctt ctttatatta ttagttctca cttgcatttt gatcatgcag gaggaaatgg
     1141 cgcttttata aatacaccaa tcattgtaca gcgtgctgaa tatgaggcgg cgcagcatag
     1201 cgaagaatat ttgaaagaat gtatattgcc gaatttaaac tacaaaatca ttgaaggtga
     1261 ttatgaagtc gtaccaggag ttcaattatt gcatacacca ggccatactc cagggcatca
     1321 atcgctatta attgagacag aaaaatccgg tcctgtatta ttaacgattg atgcatcgta
     1381 tacgaaagag aattttgaaa atgaagtgcc atttgcggga tttgattcag aattagcttt
     1441 atcttcaatt aaacgtttaa aagaagtggt gatgaaagag aagccgattg ttttctttgg
     1501 acatgatata gagcaggaaa ggggatgtaa agtgttccct gaatatatag ctgcaaacga
     1561 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga
     1621 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1681 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1741 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1801 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg
     1861 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1921 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1981 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2041 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2101 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2161 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2221 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     2281 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2341 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctgctg ctgtttaata
     2401 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2461 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2521 ggcctttctg cgtttata
//