BBa_J28019
type: ComponentDefinition
persistent identity: BBa J28019
display id: BBa_J28019
version: 1
was generated by: iGEM to SBOL conversion
description: Operon 3 (e)
created: 2006-09-04T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: soon
owned by: james
owned by: myers
mutable description: soon
discontinued: false
dominant: false
group u list: _57_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: soon
star: false
creator: Kaj Bernhardt
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_S03589
component: BBa_R0071
sequence annotation: BBa_R0071
sequence annotation: BBa_S03589
sequence: https://synbiohub.org/public/igem/BBa_J28019_sequence/1

BBa_S03589
type: Component
persistent identity: component2274520
display id: component2274520
version: 1
top level: BBa_J28019
definition: BBa_S03589
access: public

BBa_R0071
type: Component
persistent identity: component2274492
display id: component2274492
version: 1
top level: BBa_J28019
definition: RhlR+C4
access: public

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2274492
display id: annotation2274492
version: 1
top level: BBa_J28019
location: https://synbiohub.org/public/igem/BBa_J28019/annotation2274492/range2274492/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_J28019/annotation2274492/range2274492/1
type: Range
persistent identity: range2274492
display id: range2274492
version: 1
top level: BBa_J28019
direction: direction/reverse
start: 1
end: 53
orientation: inline

BBa_S03589
type: SequenceAnnotation
persistent identity: annotation2274520
display id: annotation2274520
version: 1
top level: BBa_J28019
location: https://synbiohub.org/public/igem/BBa_J28019/annotation2274520/range2274520/1
component: BBa_S03589

https://synbiohub.org/public/igem/BBa_J28019/annotation2274520/range2274520/1
type: Range
persistent identity: range2274520
display id: range2274520
version: 1
top level: BBa_J28019
direction: direction/reverse
start: 62
end: 2538
orientation: inline

BBa_S03589
type: ComponentDefinition
persistent identity: BBa S03589
display id: BBa_S03589
version: 1
was generated by: iGEM to SBOL conversion
description: S0104:S03586
created: 2006-09-04T11:00:00Z
modified: 2015-05-08T01:14:25Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _57_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Kaj Bernhardt
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S03586
component: BBa_S0104
sequence annotation: BBa_S0104
sequence annotation: BBa_S03586
sequence: https://synbiohub.org/public/igem/BBa_S03589_sequence/1

BBa_S03586
type: Component
persistent identity: component2269677
display id: component2269677
version: 1
top level: BBa_S03589
definition: BBa_S03586
access: public

BBa_S0104
type: Component
persistent identity: component2269657
display id: component2269657
version: 1
top level: BBa_S03589
definition: BBa_S0104
access: public

BBa_S0104
type: SequenceAnnotation
persistent identity: annotation2269657
display id: annotation2269657
version: 1
top level: BBa_S03589
location: https://synbiohub.org/public/igem/BBa_S03589/annotation2269657/range2269657/1
component: BBa_S0104

https://synbiohub.org/public/igem/BBa_S03589/annotation2269657/range2269657/1
type: Range
persistent identity: range2269657
display id: range2269657
version: 1
top level: BBa_S03589
direction: direction/reverse
start: 1
end: 712
orientation: inline

BBa_S03586
type: SequenceAnnotation
persistent identity: annotation2269677
display id: annotation2269677
version: 1
top level: BBa_S03589
location: https://synbiohub.org/public/igem/BBa_S03589/annotation2269677/range2269677/1
component: BBa_S03586

https://synbiohub.org/public/igem/BBa_S03589/annotation2269677/range2269677/1
type: Range
persistent identity: range2269677
display id: range2269677
version: 1
top level: BBa_S03589
direction: direction/reverse
start: 721
end: 2477
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (RhlR & C4-HSL regulated)
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
top level: RhlR+C4
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
top level: RhlR+C4
direction: direction/forward
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
top level: RhlR+C4
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
top level: RhlR+C4
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_S03586
type: ComponentDefinition
persistent identity: BBa S03586
display id: BBa_S03586
version: 1
was generated by: iGEM to SBOL conversion
description: C0260:E0434
created: 2006-09-04T11:00:00Z
modified: 2015-05-08T01:14:25Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _57_
m user id: 0
owner id: 1072
owning group id: 57
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Kaj Bernhardt
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_E0434
component: BBa_C0260
sequence annotation: BBa_C0260
sequence annotation: BBa_E0434
sequence: https://synbiohub.org/public/igem/BBa_S03586_sequence/1

BBa_E0434
type: Component
persistent identity: component2260229
display id: component2260229
version: 1
top level: BBa_S03586
definition: BBa_E0434
access: public

BBa_C0260
type: Component
persistent identity: component2260219
display id: component2260219
version: 1
top level: BBa_S03586
definition: BBa_C0260
access: public

BBa_C0260
type: SequenceAnnotation
persistent identity: annotation2260219
display id: annotation2260219
version: 1
top level: BBa_S03586
location: https://synbiohub.org/public/igem/BBa_S03586/annotation2260219/range2260219/1
component: BBa_C0260

https://synbiohub.org/public/igem/BBa_S03586/annotation2260219/range2260219/1
type: Range
persistent identity: range2260219
display id: range2260219
version: 1
top level: BBa_S03586
direction: direction/reverse
start: 1
end: 832
orientation: inline

BBa_E0434
type: SequenceAnnotation
persistent identity: annotation2260229
display id: annotation2260229
version: 1
top level: BBa_S03586
location: https://synbiohub.org/public/igem/BBa_S03586/annotation2260229/range2260229/1
component: BBa_E0434

https://synbiohub.org/public/igem/BBa_S03586/annotation2260229/range2260229/1
type: Range
persistent identity: range2260229
display id: range2260229
version: 1
top level: BBa_S03586
direction: direction/reverse
start: 841
end: 1757
orientation: inline

BBa_S0104
type: ComponentDefinition
persistent identity: BBa S0104
display id: BBa_S0104
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.C0052
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0052
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_S0104_sequence/1

BBa_C0052
type: Component
persistent identity: component939772
display id: component939772
version: 1
top level: BBa_S0104
definition: cI 434
access: public

BBa_B0034
type: Component
persistent identity: component939762
display id: component939762
version: 1
top level: BBa_S0104
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939762
display id: annotation939762
version: 1
top level: BBa_S0104
location: https://synbiohub.org/public/igem/BBa_S0104/annotation939762/range939762/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0104/annotation939762/range939762/1
type: Range
persistent identity: range939762
display id: range939762
version: 1
top level: BBa_S0104
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation939772
display id: annotation939772
version: 1
top level: BBa_S0104
location: https://synbiohub.org/public/igem/BBa_S0104/annotation939772/range939772/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_S0104/annotation939772/range939772/1
type: Range
persistent identity: range939772
display id: range939772
version: 1
top level: BBa_S0104
direction: direction/forward
start: 19
end: 687
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_C0260
type: ComponentDefinition
persistent identity: BBa C0260
display id: BBa_C0260
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme, AaiiA (+RBS)
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
mutable description: B0034.C0060
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: robertb
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_C0060
sequence annotation: BBa_B0034
sequence annotation: BBa_C0060
sequence: https://synbiohub.org/public/igem/BBa_C0260_sequence/1

BBa_B0034
type: Component
persistent identity: component1060382
display id: component1060382
version: 1
top level: BBa_C0260
definition: BBa_B0034
access: public

BBa_C0060
type: Component
persistent identity: component1060397
display id: component1060397
version: 1
top level: BBa_C0260
definition: aiiA
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060382
display id: annotation1060382
version: 1
top level: BBa_C0260
location: https://synbiohub.org/public/igem/BBa_C0260/annotation1060382/range1060382/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0260/annotation1060382/range1060382/1
type: Range
persistent identity: range1060382
display id: range1060382
version: 1
top level: BBa_C0260
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1060397
display id: annotation1060397
version: 1
top level: BBa_C0260
location: https://synbiohub.org/public/igem/BBa_C0260/annotation1060397/range1060397/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_C0260/annotation1060397/range1060397/1
type: Range
persistent identity: range1060397
display id: range1060397
version: 1
top level: BBa_C0260
direction: direction/forward
start: 19
end: 807
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0052
sequence annotation: cI 434
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
top level: cI 434
direction: direction/forward
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
top level: cI 434
direction: direction/forward
start: 670
end: 694
orientation: inline

BBa_E0434
type: ComponentDefinition
persistent identity: BBa E0434
display id: BBa_E0434
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.E0034.B0015
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
mutable description: B0034.E0034.B0015 output device with aav tagged yfp
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: jcbraff
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0034
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0434_sequence/1

BBa_E0034
type: Component
persistent identity: component942990
display id: component942990
version: 1
top level: BBa_E0434
definition: EYFP
access: public

BBa_B0012
type: Component
persistent identity: component943005
display id: component943005
version: 1
top level: BBa_E0434
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component942988
display id: component942988
version: 1
top level: BBa_E0434
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component942995
display id: component942995
version: 1
top level: BBa_E0434
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942988
display id: annotation942988
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation942988/range942988/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0434/annotation942988/range942988/1
type: Range
persistent identity: range942988
display id: range942988
version: 1
top level: BBa_E0434
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation942990
display id: annotation942990
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation942990/range942990/1
component: BBa_E0034

https://synbiohub.org/public/igem/BBa_E0434/annotation942990/range942990/1
type: Range
persistent identity: range942990
display id: range942990
version: 1
top level: BBa_E0434
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942995
display id: annotation942995
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation942995/range942995/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0434/annotation942995/range942995/1
type: Range
persistent identity: range942995
display id: range942995
version: 1
top level: BBa_E0434
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943005
display id: annotation943005
version: 1
top level: BBa_E0434
location: https://synbiohub.org/public/igem/BBa_E0434/annotation943005/range943005/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0434/annotation943005/range943005/1
type: Range
persistent identity: range943005
display id: range943005
version: 1
top level: BBa_E0434
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: start
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
top level: aiiA
direction: direction/forward
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
top level: aiiA
direction: direction/forward
start: 784
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
top level: aiiA
direction: direction/forward
start: 790
end: 814
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: AAV
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
top level: EYFP
direction: direction/forward
start: 718
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
top level: EYFP
direction: direction/forward
start: 757
end: 762
orientation: inline

https://synbiohub.org/public/igem/BBa_J28019_sequence/1
type: Sequence
persistent identity: BBa J28019 sequence
display id: BBa_J28019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03589_sequence/1
type: Sequence
persistent identity: BBa S03589 sequence
display id: BBa_S03589_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0071_sequence/1
type: Sequence
persistent identity: BBa R0071 sequence
display id: BBa_R0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03586_sequence/1
type: Sequence
persistent identity: BBa S03586 sequence
display id: BBa_S03586_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S0104_sequence/1
type: Sequence
persistent identity: BBa S0104 sequence
display id: BBa_S0104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0260_sequence/1
type: Sequence
persistent identity: BBa C0260 sequence
display id: BBa_C0260_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0052_sequence/1
type: Sequence
persistent identity: BBa C0052 sequence
display id: BBa_C0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0434_sequence/1
type: Sequence
persistent identity: BBa E0434 sequence
display id: BBa_E0434_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0060_sequence/1
type: Sequence
persistent identity: BBa C0060 sequence
display id: BBa_C0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0034_sequence/1
type: Sequence
persistent identity: BBa E0034 sequence
display id: BBa_E0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z