LOCUS       BBa_J28020              2710 bp    DNA     linear       10-Jun-2024
DEFINITION  Operon 3 (f)
ACCESSION   BBa_J28020
VERSION     BBa_J28020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..220
                     /label=unidentified/uncharacterized CinR dependent
     stem_loop       35..74
                     /label=stem_loop
     stem_loop       91..136
                     /label=stem_loop
     promoter        1..225
                     /label=BBa_R0078
     misc_feature    238..241
                     /label=conserved
     RBS             234..245
                     /label=BBa_B0034
     CDS             252..920
                     /label=cI 434
     misc_feature    882..920
                     /label=LVA
     misc_feature    252..920
                     /label=BBa_C0052
     misc_feature    921..945
                     /label=Help:Barcodes
     CDS             252..920
                     /label=BBa_C0052
     misc_feature    234..945
                     /label=BBa_S0104
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     misc_feature    972..974
                     /label=start
     misc_feature    1755..1760
                     /label=2
     misc_feature    1722..1754
                     /label=LVA
     CDS             972..1721
                     /label=aiiA
     misc_feature    972..1760
                     /label=BBa_C0060
     misc_feature    1761..1785
                     /label=Help:Barcodes
     CDS             972..1760
                     /label=BBa_C0060
     misc_feature    954..1785
                     /label=BBa_C0260
     misc_feature    1798..1801
                     /label=conserved
     RBS             1794..1805
                     /label=BBa_B0034
     misc_feature    2568..2573
                     /label=stop
     misc_feature    2529..2567
                     /label=AAV
     misc_feature    1812..2573
                     /label=BBa_E0034
     stem_loop       2593..2636
                     /label=stem_loop
     misc_feature    2582..2661
                     /label=BBa_B0010
     terminator      2582..2661
                     /label=BBa_B0010
     stem_loop       2677..2696
                     /label=T7 TE
     misc_feature    2703..2703
                     /label=stop
     polyA_site      2697..2710
                     /label=polya
     misc_feature    2670..2710
                     /label=BBa_B0012
     terminator      2670..2710
                     /label=BBa_B0012
     misc_feature    1794..2710
                     /label=BBa_E0434
     misc_feature    954..2710
                     /label=BBa_S03586
     misc_feature    234..2710
                     /label=BBa_S03589
ORIGIN
        1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc
       61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac
      121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga
      181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctacta gagaaagagg
      241 agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag cttggactta
      301 accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag cagctcgaaa
      361 acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt ggcgtaagtg
      421 ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg cacgttgagc
      481 ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt gaagcttgtg
      541 aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac ttattaggca
      601 atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt caaagcatcc
      661 ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga agccttgttg
      721 tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata gatggcggtc
      781 agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac ggaaactgca
      841 agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac gacgaaaact
      901 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagaaagagg
      961 agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg
     1021 atcattcgtc tgttaatagt acattaacac caggagaatt attagactta ccggtttggt
     1081 gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg
     1141 cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa
     1201 tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc
     1261 ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta
     1321 taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat
     1381 atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag
     1441 tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat
     1501 taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag
     1561 agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa
     1621 ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata
     1681 tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact
     1741 acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta gagaaagagg
     1801 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1861 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1921 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1981 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg
     2041 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2101 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2161 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2221 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     2281 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     2341 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2401 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2461 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2521 tgtacaagag gcctgctgca aacgacgaaa actacgctgc tgctgtttaa taatactaga
     2581 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2641 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2701 tgcgtttata
//