LOCUS BBa_J28021 1802 bp DNA linear 25-Nov-2024 DEFINITION Operon 3 (g) ACCESSION BBa_J28021 VERSION BBa_J28021.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 CDS 184..852 /label=cI 434 misc_feature 814..852 /label=LVA misc_feature 184..852 /label=BBa_C0052 misc_feature 853..877 /label=Help:Barcodes CDS 184..852 /label=BBa_C0052 misc_feature 166..877 /label=BBa_S0104 misc_feature 890..893 /label=conserved RBS 886..897 /label=BBa_B0034 misc_feature 1660..1665 /label=stop misc_feature 1621..1659 /label=AAV misc_feature 904..1665 /label=BBa_E0034 stem_loop 1685..1728 /label=stem_loop misc_feature 1674..1753 /label=BBa_B0010 terminator 1674..1753 /label=BBa_B0010 stem_loop 1769..1788 /label=T7 TE misc_feature 1795..1795 /label=stop polyA_site 1789..1802 /label=polya misc_feature 1762..1802 /label=BBa_B0012 terminator 1762..1802 /label=BBa_B0012 misc_feature 886..1802 /label=BBa_E0434 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatgagta tttcttccag ggtaaaaagc aaaagaatcc agcttggact taaccaggct 241 gaacttgctc aaaaggtggg gactacccag cagtctatag agcagctcga aaacggtaaa 301 actaagcgac cacgcttttt accagaactt gcgtcagctc ttggcgtaag tgttgactgg 361 ctgctcaatg gcacctctga ttcgaatgtt agatttgttg ggcacgttga gcccaaaggg 421 aaatatccat tgattagcat ggttagagct ggttcgtggt gtgaagcttg tgaaccctac 481 gatatcaagg acattgatga atggtatgac agtgacgtta acttattagg caatggattc 541 tggctgaagg ttgaaggtga ttccatgacc tcacctgtag gtcaaagcat ccctgaaggt 601 catatggtgt tagtagatac tggacgggag ccagtgaatg gaagccttgt tgtagccaaa 661 ctgactgacg cgaacgaagc aacattcaag aaactggtca tagatggcgg tcagaagtac 721 ctgaaaggcc tgaatccttc atggcctatg actcctatca acggaaactg caagattatc 781 ggtgttgtcg tggaagcgag ggtaaaattc gtagctgcaa acgacgaaaa ctacgcttta 841 gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac 901 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 961 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 1021 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 1081 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg 1141 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 1201 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 1261 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 1321 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 1381 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 1441 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 1501 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 1561 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 1621 aggcctgctg caaacgacga aaactacgct gctgctgttt aataatacta gagccaggca 1681 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1741 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1801 ta //