LOCUS       BBa_J28022              1781 bp    DNA     linear       12-Mar-2025
DEFINITION  Operon 4 (b)
ACCESSION   BBa_J28022
VERSION     BBa_J28022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..831
                     /label=BBa_C0051
     misc_feature    64..856
                     /label=BBa_J06550
     misc_feature    869..872
                     /label=conserved
     RBS             865..876
                     /label=BBa_B0034
     CDS             883..1644
                     /label=CFP (LVA)
     misc_feature    1601..1638
                     /label=SsrA
     misc_feature    1639..1644
                     /label=2
     misc_feature    951..951
                     /label=A
     misc_feature    883..1644
                     /label=BBa_E0022
     misc_feature    883..1644
                     /label=BBa_E0022
     stem_loop       1664..1707
                     /label=stem_loop
     misc_feature    1653..1732
                     /label=BBa_B0010
     terminator      1653..1732
                     /label=BBa_B0010
     stem_loop       1748..1767
                     /label=T7 TE
     misc_feature    1774..1774
                     /label=stop
     polyA_site      1768..1781
                     /label=polya
     misc_feature    1741..1781
                     /label=BBa_B0012
     terminator      1741..1781
                     /label=BBa_B0012
     misc_feature    865..1781
                     /label=BBa_E0422
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagaaagag gagaaatact agatggtgag caagggcgag
      901 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac
      961 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1021 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1081 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1141 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1201 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1261 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1321 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     1381 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1441 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1501 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1561 gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctgctgc aaacgacgaa
     1621 aactacgctt tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca
     1681 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1741 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//