LOCUS BBa_J28024 4805 bp DNA linear 22-Nov-2024 DEFINITION Intermediate System Part 1 of 2 ACCESSION BBa_J28024 VERSION BBa_J28024.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 157..158 /label=prefix misc_feature 330..330 /label=T misc_feature 648..648 /label=A CDS 157..906 /label=luxR misc_feature 157..912 /label=BBa_C0062 misc_feature 913..937 /label=Help:Barcodes CDS 157..912 /label=BBa_C0062 stem_loop 957..1000 /label=stem_loop misc_feature 946..1025 /label=BBa_B0010 terminator 946..1025 /label=BBa_B0010 stem_loop 1041..1060 /label=T7 TE misc_feature 1067..1067 /label=stop polyA_site 1061..1074 /label=polya misc_feature 1034..1074 /label=BBa_B0012 terminator 1034..1074 /label=BBa_B0012 terminator 946..1074 /label=BBa_B0015 misc_feature 1083..1102 /label=LuxR/HSL promoter 1102..1107 /label=-35 promoter 1124..1129 /label=-10 misc_feature 1135..1135 /label=start misc_feature 1083..1137 /label=BBa_R0062 promoter 1083..1137 /label=BBa_R0062 misc_feature 1150..1153 /label=conserved RBS 1146..1157 /label=BBa_B0034 misc_feature 1743..1774 /label=LVA CDS 1164..1742 /label=luxI misc_feature 1164..1781 /label=BBa_C0061 misc_feature 1782..1806 /label=Help:Barcodes CDS 1164..1781 /label=BBa_C0061 misc_feature 1819..1822 /label=conserved RBS 1815..1826 /label=BBa_B0034 CDS 1833..2507 /label=mrfp1 misc_feature 2514..2538 /label=Help:Barcodes CDS 1833..2538 /label=BBa_E1010 stem_loop 2558..2601 /label=stem_loop misc_feature 2547..2626 /label=BBa_B0010 terminator 2547..2626 /label=BBa_B0010 stem_loop 2642..2661 /label=T7 TE misc_feature 2668..2668 /label=stop polyA_site 2662..2675 /label=polya misc_feature 2635..2675 /label=BBa_B0012 terminator 2635..2675 /label=BBa_B0012 terminator 2547..2675 /label=BBa_B0015 promoter 2684..2813 /label=BBa_I13453 misc_feature 2826..2829 /label=conserved RBS 2822..2833 /label=BBa_B0034 CDS 2840..3556 /label=lasR misc_feature 3557..3589 /label=LVA misc_feature 3319..3319 /label=G misc_feature 3596..3620 /label=Help:Barcodes CDS 2840..3620 /label=BBa_C0079 stem_loop 3640..3683 /label=stem_loop misc_feature 3629..3708 /label=BBa_B0010 terminator 3629..3708 /label=BBa_B0010 stem_loop 3724..3743 /label=T7 TE misc_feature 3750..3750 /label=stop polyA_site 3744..3757 /label=polya misc_feature 3717..3757 /label=BBa_B0012 terminator 3717..3757 /label=BBa_B0012 terminator 3629..3757 /label=BBa_B0015 misc_feature 3811..3828 /label=OP2 misc_feature 3871..3890 /label=OP1 promoter 3882..3887 /label=-35 promoter 3905..3910 /label=-10 promoter 3766..3922 /label=BBa_R0079 misc_feature 3935..3938 /label=conserved RBS 3931..3942 /label=BBa_B0034 CDS 3949..4668 /label=GFP protein CDS 3949..4668 /label=BBa_E0040 stem_loop 4688..4731 /label=stem_loop misc_feature 4677..4756 /label=BBa_B0010 terminator 4677..4756 /label=BBa_B0010 stem_loop 4772..4791 /label=T7 TE misc_feature 4798..4798 /label=stop polyA_site 4792..4805 /label=polya misc_feature 4765..4805 /label=BBa_B0012 terminator 4765..4805 /label=BBa_B0012 terminator 4677..4805 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac 181 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc 241 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct 301 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa 361 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat 421 cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat 481 gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg 541 gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat 601 agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat 661 tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa 721 gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt 781 tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca 841 aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt 901 aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa 961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1081 agacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac 1141 tagagaaaga ggagaaatac tagatgacta taatgataaa aaaatcggat tttttggcaa 1201 ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac 1261 ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat aactcaaatg 1321 cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg cgtttattac 1381 ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt caacagagtg 1441 ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa 1501 agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata tataaacacg 1561 ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata gagcgatttt 1621 taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat gtattaggtg 1681 atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa 1741 atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta 1801 gatctctact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag 1861 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg 1921 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca 1981 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca 2041 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag 2101 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg 2161 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc 2221 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta 2281 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg 2341 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc 2401 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca 2461 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat 2521 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2581 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2641 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacattga ttatttgcac 2701 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga 2761 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga 2821 gaaagaggag aaatactaga tggccttggt tgacggtttt cttgagctgg aacgctcaag 2881 tggaaaattg gagtggagcg ccatcctcca gaagatggcg agcgaccttg gattctcgaa 2941 gatcctgttc ggcctgttgc ctaaggacag ccaggactac gagaacgcct tcatcgtcgg 3001 caactacccg gccgcctggc gcgagcatta cgaccgggct ggctacgcgc gggtcgaccc 3061 gacggtcagt cactgtaccc agagcgtact gccgattttc tgggaaccgt ccatctacca 3121 gacgcgaaag cagcacgagt tcttcgagga agcctcggcc gccggcctgg tgtatgggct 3181 gaccatgccg ctgcatggtg ctcgcggcga actcggcgcg ctgagcctca gcgtggaagc 3241 ggaaaaccgg gccgaggcca accgtttcat agagtcggtc ctgccgaccc tgtggatgct 3301 caaggactac gcactgcaaa gcggtgccgg actggccttc gaacatccgg tcagcaaacc 3361 ggtggttctg accagccggg agaaggaagt gttgcagtgg tgcgccatcg gcaagaccag 3421 ttgggagata tcggttatct gcaactgctc ggaagccaat gtgaacttcc atatgggaaa 3481 tattcggcgg aagttcggtg tgacctcccg ccgcgtagcg gccattatgg ccgttaattt 3541 gggtcttatt actctcgctg caaacgacga aaactacgct ttagtagctt aataacactg 3601 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3661 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3721 actggctcac cttcgggtgg gcctttctgc gtttatatac tagaggcccc tcgctgagcg 3781 cgtcccggag ctgggggcaa cctagctgcc acctgctttt ctgctagcta ttccagcgaa 3841 aacatacaga tttccggcga aatcaaggct acctgccagt tctggcaggt ttggccgcgg 3901 gttctttttg gtacacgaaa gctactagag aaagaggaga aatactagat gcgtaaagga 3961 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 4021 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 4081 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 4141 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc 4201 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 4261 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag 4321 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac 4381 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac 4441 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa 4501 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 4561 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 4621 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag 4681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 4741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4801 ttata //