##gff-version 3
##sequence-region BBa_J28024 1 4805
BBa_J28024	.	CDS	1164	1781	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J28024	.	CDS	1164	1742	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J28024	.	tag	1743	1774	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J28024	.	engineered_tag	1782	1806	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J28024	.	engineered_region	1164	1781	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J28024	.	ribosome_entry_site	1815	1826	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28024	.	conserved_region	1819	1822	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28024	.	promoter	1083	1137	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J28024	.	promoter	1102	1107	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J28024	.	promoter	1124	1129	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J28024	.	engineered_region	1083	1137	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J28024	.	start_codon	1135	1135	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J28024	.	non_covalent_binding_site	1083	1102	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J28024	.	terminator	2547	2675	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28024	.	terminator	2635	2675	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28024	.	polyA_site	2662	2675	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28024	.	engineered_region	2635	2675	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28024	.	stem_loop	2642	2661	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28024	.	stop_codon	2668	2668	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28024	.	terminator	2547	2626	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28024	.	stem_loop	2558	2601	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28024	.	engineered_region	2547	2626	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28024	.	ribosome_entry_site	2822	2833	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28024	.	conserved_region	2826	2829	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28024	.	terminator	946	1074	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28024	.	terminator	1034	1074	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28024	.	polyA_site	1061	1074	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28024	.	engineered_region	1034	1074	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28024	.	stem_loop	1041	1060	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28024	.	stop_codon	1067	1067	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28024	.	terminator	946	1025	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28024	.	stem_loop	957	1000	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28024	.	engineered_region	946	1025	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28024	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_J28024	.	terminator	4677	4805	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28024	.	terminator	4765	4805	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28024	.	polyA_site	4792	4805	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28024	.	engineered_region	4765	4805	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28024	.	stem_loop	4772	4791	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28024	.	stop_codon	4798	4798	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28024	.	terminator	4677	4756	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28024	.	stem_loop	4688	4731	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28024	.	engineered_region	4677	4756	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28024	.	ribosome_entry_site	1146	1157	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28024	.	conserved_region	1150	1153	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28024	.	CDS	1833	2538	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J28024	.	CDS	1833	2507	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J28024	.	engineered_tag	2514	2538	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J28024	.	terminator	3629	3757	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28024	.	terminator	3717	3757	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28024	.	polyA_site	3744	3757	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28024	.	engineered_region	3717	3757	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28024	.	stem_loop	3724	3743	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28024	.	stop_codon	3750	3750	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28024	.	terminator	3629	3708	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28024	.	stem_loop	3640	3683	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28024	.	engineered_region	3629	3708	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28024	.	promoter	2684	2813	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_J28024	.	CDS	157	912	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J28024	.	point_mutation	648	648	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J28024	.	engineered_region	157	912	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J28024	.	engineered_tag	913	937	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J28024	.	CDS	157	906	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J28024	.	point_mutation	330	330	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J28024	.	engineered_region	157	158	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J28024	.	CDS	2840	3620	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_J28024	.	engineered_tag	3596	3620	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_J28024	.	tag	3557	3589	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_J28024	.	CDS	2840	3556	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_J28024	.	sequence_alteration	3319	3319	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_J28024	.	ribosome_entry_site	3931	3942	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28024	.	conserved_region	3935	3938	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28024	.	CDS	3949	4668	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J28024	.	CDS	3949	4668	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J28024	.	promoter	3766	3922	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_J28024	.	promoter	3905	3910	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_J28024	.	promoter	3882	3887	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_J28024	.	non_covalent_binding_site	3811	3828	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_J28024	.	non_covalent_binding_site	3871	3890	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_J28024	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28024	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J28024
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa
tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat
ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc
tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct
ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct
tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc
attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa
agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc
gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat
tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttac
gcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgactataatgataaaaaaat
cggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttga
gtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgat
gatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaat
tgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaa
gataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattaca
gaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagaca
aagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtctt
aaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaa
gaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccg
ttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagt
taccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaa
cacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaag
acggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtac
caacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaa
gacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacca
cctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacga
agactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctag
tgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaca
ttgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctt
tttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggc
cttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagc
gaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggca
actacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtaccca
gagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggcc
gccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaag
cggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgca
aagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcag
tggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgg
gaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactct
cgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagct
gggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggcta
cctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatactagat
gcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattt
tctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactac
ctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaaca
gcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactac
aagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaag
atggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaa
tggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccca
acgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaa
ataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata