##gff-version 3
##sequence-region BBa_J28025 1 4849
BBa_J28025	.	CDS	157	937	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_J28025	.	engineered_tag	913	937	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_J28025	.	tag	874	906	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_J28025	.	sequence_alteration	636	636	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_J28025	.	CDS	157	873	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_J28025	.	terminator	2690	2818	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28025	.	terminator	2690	2769	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28025	.	stem_loop	2701	2744	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28025	.	engineered_region	2690	2769	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28025	.	terminator	2778	2818	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28025	.	engineered_region	2778	2818	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28025	.	stop_codon	2811	2811	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28025	.	polyA_site	2805	2818	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28025	.	stem_loop	2785	2804	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28025	.	promoter	1083	1239	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_J28025	.	non_covalent_binding_site	1188	1207	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_J28025	.	promoter	1199	1204	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_J28025	.	non_covalent_binding_site	1128	1145	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_J28025	.	promoter	1222	1227	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_J28025	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28025	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28025	.	ribosome_entry_site	2965	2976	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28025	.	conserved_region	2969	2972	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28025	.	ribosome_entry_site	1248	1259	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28025	.	conserved_region	1252	1255	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28025	.	terminator	3772	3900	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28025	.	terminator	3772	3851	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28025	.	stem_loop	3783	3826	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28025	.	engineered_region	3772	3851	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28025	.	terminator	3860	3900	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28025	.	engineered_region	3860	3900	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28025	.	stop_codon	3893	3893	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28025	.	polyA_site	3887	3900	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28025	.	stem_loop	3867	3886	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28025	.	CDS	1959	2681	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_J28025	.	CDS	2983	3738	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J28025	.	point_mutation	3474	3474	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J28025	.	engineered_region	2983	3738	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J28025	.	engineered_region	2983	2984	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J28025	.	CDS	2983	3732	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J28025	.	point_mutation	3156	3156	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J28025	.	engineered_tag	3739	3763	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J28025	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_J28025	.	terminator	4721	4849	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28025	.	terminator	4721	4800	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28025	.	stem_loop	4732	4775	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28025	.	engineered_region	4721	4800	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28025	.	terminator	4809	4849	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28025	.	engineered_region	4809	4849	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28025	.	stop_codon	4842	4842	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28025	.	polyA_site	4836	4849	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28025	.	stem_loop	4816	4835	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28025	.	ribosome_entry_site	3972	3983	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28025	.	conserved_region	3976	3979	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28025	.	promoter	3909	3963	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J28025	.	promoter	3928	3933	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J28025	.	engineered_region	3909	3963	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J28025	.	start_codon	3961	3961	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J28025	.	promoter	3950	3955	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J28025	.	non_covalent_binding_site	3909	3928	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J28025	.	promoter	2827	2956	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_J28025	.	CDS	3990	4712	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J28025	.	ribosome_entry_site	1941	1952	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28025	.	conserved_region	1945	1948	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28025	.	terminator	946	1074	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28025	.	terminator	946	1025	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28025	.	stem_loop	957	1000	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28025	.	engineered_region	946	1025	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28025	.	terminator	1034	1074	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28025	.	engineered_region	1034	1074	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28025	.	stop_codon	1067	1067	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28025	.	polyA_site	1061	1074	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28025	.	stem_loop	1041	1060	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28025	.	CDS	1266	1932	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_J28025	.	CDS	1266	1868	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_J28025	.	engineered_tag	1908	1932	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_J28025	.	tag	1869	1901	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
>BBa_J28025
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcg
agcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcg
gcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtac
ccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcg
gccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtgg
aagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcact
gcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttg
cagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccata
tgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattac
tctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccgga
gctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaagg
ctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatacta
gatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggtt
ttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtact
acatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacat
gctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttc
gctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttact
ccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctgga
cgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacc
cagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctgg
ttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcga
ggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggc
gagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggc
ccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttctt
caagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgcc
gaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcc
tggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggc
caacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggc
gacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcg
atcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacg
gcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctc
tactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacataaatgccga
cgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgact
aaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctag
ataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaa
ctccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaa
gcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttg
cacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctct
agttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgttta
gcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatt
taaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattga
ttgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgtt
atagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggc
tacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata