LOCUS       BBa_J28029              1993 bp    DNA     linear       22-Dec-2024
DEFINITION  las autoinducer, P(tet)/tetR control
ACCESSION   BBa_J28029
VERSION     BBa_J28029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..797
                     /label=lasR
     misc_feature    798..830
                     /label=LVA
     misc_feature    560..560
                     /label=G
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..861
                     /label=BBa_C0079
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1052..1069
                     /label=OP2
     misc_feature    1112..1131
                     /label=OP1
     promoter        1123..1128
                     /label=-35
     promoter        1146..1151
                     /label=-10
     promoter        1007..1163
                     /label=BBa_R0079
     misc_feature    1176..1179
                     /label=conserved
     RBS             1172..1183
                     /label=BBa_B0034
     CDS             1190..1792
                     /label=LasI
     misc_feature    1793..1825
                     /label=LVA
     misc_feature    1832..1856
                     /label=Help:Barcodes
     CDS             1190..1856
                     /label=BBa_C0078
     stem_loop       1876..1919
                     /label=stem_loop
     misc_feature    1865..1944
                     /label=BBa_B0010
     terminator      1865..1944
                     /label=BBa_B0010
     stem_loop       1960..1979
                     /label=T7 TE
     misc_feature    1986..1986
                     /label=stop
     polyA_site      1980..1993
                     /label=polya
     misc_feature    1953..1993
                     /label=BBa_B0012
     terminator      1953..1993
                     /label=BBa_B0012
     terminator      1865..1993
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
      121 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
      181 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
      241 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
      301 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
      361 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
      421 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
      481 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
      541 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
      601 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
      661 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
      721 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
      781 tgggtcttat tactctcgct gcaaacgacg aaaactacgc tttagtagct taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagaggccc ctcgctgagc
     1021 gcgtcccgga gctgggggca acctagctgc cacctgcttt tctgctagct attccagcga
     1081 aaacatacag atttccggcg aaatcaaggc tacctgccag ttctggcagg tttggccgcg
     1141 ggttcttttt ggtacacgaa agctactaga gaaagaggag aaatactaga tgatcgttca
     1201 gatcggtcgt cgtgaagagt tcgacaaaaa actgctgggt gaaatgcaca aactgcgtgc
     1261 tcaggttttc aaagaacgta aaggttggga cgtttccgtt atcgacgaaa tggaaatcga
     1321 cggttacgac gctctgtccc cgtactacat gctgatccag gaagacaccc cggaagctca
     1381 ggttttcggt tgctggcgta tcttcgacac caccggtccg tacatgctga aaaacacctt
     1441 cccggaactg ctgcacggta aagaagctcc gtgctccccg cacatctggg aactgtcccg
     1501 tttcgctatc aactccggtc agaaaggttc cctgggtttc tccgactgca ccctggaagc
     1561 tatgcgtgct ctggctcgtt actccttgca gaacgacatc cagaccctgg ttaccgttac
     1621 caccgttggt gttgaaaaaa tgatgatccg tgctggtctg gacgtttccc gtttcggtcc
     1681 gcacctgaaa atcggtatcg aacgtgctgt tgctctgcgt atcgaactga acgctaaaac
     1741 ccagatcgct ctgtacggtg gtgttctggt tgaacagcgt ctggctgttt ccgctgctaa
     1801 cgacgaaaac tacgctctgg ttgcttaata actctgatag tgctagtgta gatctctact
     1861 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1981 ttctgcgttt ata
//