##gff-version 3
##sequence-region BBa_J28029 1 1993
BBa_J28029	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28029	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28029	.	ribosome_entry_site	1172	1183	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28029	.	conserved_region	1176	1179	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28029	.	terminator	1865	1993	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28029	.	terminator	1865	1944	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28029	.	engineered_region	1865	1944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28029	.	stem_loop	1876	1919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28029	.	terminator	1953	1993	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28029	.	polyA_site	1980	1993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28029	.	stem_loop	1960	1979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28029	.	engineered_region	1953	1993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28029	.	stop_codon	1986	1986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28029	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28029	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28029	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28029	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28029	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28029	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28029	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28029	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28029	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28029	.	promoter	1007	1163	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_J28029	.	non_covalent_binding_site	1112	1131	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_J28029	.	promoter	1123	1128	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_J28029	.	non_covalent_binding_site	1052	1069	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_J28029	.	promoter	1146	1151	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_J28029	.	CDS	1190	1856	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_J28029	.	tag	1793	1825	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_J28029	.	engineered_tag	1832	1856	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_J28029	.	CDS	1190	1792	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_J28029	.	CDS	81	861	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_J28029	.	sequence_alteration	560	560	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_J28029	.	tag	798	830	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_J28029	.	CDS	81	797	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_J28029	.	engineered_tag	837	861	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_J28029	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J28029	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J28029	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J28029	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J28029	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J28029	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_J28029
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatg
gcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcg
tcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactg
tacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcc
tcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcg
tggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgc
actgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtg
ttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttcc
atatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttat
tactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtccc
ggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatca
aggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaata
ctagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcag
gttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgt
actacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgta
catgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgt
ttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgtt
actccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtct
ggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaa
acccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctc
tggttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata