##gff-version 3
##sequence-region BBa_J28030 1 2069
BBa_J28030	.	CDS	157	937	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_J28030	.	tag	874	906	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_J28030	.	CDS	157	873	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_J28030	.	engineered_tag	913	937	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_J28030	.	sequence_alteration	636	636	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_J28030	.	ribosome_entry_site	1248	1259	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28030	.	conserved_region	1252	1255	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28030	.	terminator	1941	2069	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28030	.	terminator	2029	2069	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28030	.	stem_loop	2036	2055	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28030	.	engineered_region	2029	2069	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28030	.	stop_codon	2062	2062	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28030	.	polyA_site	2056	2069	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28030	.	terminator	1941	2020	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28030	.	stem_loop	1952	1995	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28030	.	engineered_region	1941	2020	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28030	.	terminator	946	1074	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J28030	.	terminator	1034	1074	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J28030	.	stem_loop	1041	1060	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28030	.	engineered_region	1034	1074	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28030	.	stop_codon	1067	1067	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28030	.	polyA_site	1061	1074	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28030	.	terminator	946	1025	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J28030	.	stem_loop	957	1000	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28030	.	engineered_region	946	1025	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28030	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_J28030	.	promoter	1083	1239	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_J28030	.	non_covalent_binding_site	1188	1207	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_J28030	.	promoter	1199	1204	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_J28030	.	non_covalent_binding_site	1128	1145	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_J28030	.	promoter	1222	1227	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_J28030	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J28030	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28030	.	CDS	1266	1932	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_J28030	.	tag	1869	1901	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_J28030	.	CDS	1266	1868	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_J28030	.	engineered_tag	1908	1932	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
>BBa_J28030
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcg
agcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcg
gcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtac
ccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcg
gccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtgg
aagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcact
gcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttg
cagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccata
tgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattac
tctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccgga
gctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaagg
ctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatacta
gatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggtt
ttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtact
acatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacat
gctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttc
gctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttact
ccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctgga
cgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacc
cagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctgg
ttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata