LOCUS       BBa_J29006              2235 bp    DNA     linear       22-Nov-2024
DEFINITION  CrtB protein generator
ACCESSION   BBa_J29006
VERSION     BBa_J29006.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1225..1228
                     /label=conserved
     RBS             1219..1232
                     /label=BBa_B0031
     misc_feature    2127..2132
                     /label=double stop codon
     CDS             1239..2132
                     /label=crtB coding sequence
     CDS             1239..2132
                     /label=BBa_J29001
     stem_loop       2148..2167
                     /label=T7 TE
     misc_feature    2174..2174
                     /label=stop
     polyA_site      2168..2181
                     /label=polya
     misc_feature    2141..2181
                     /label=BBa_B0012
     terminator      2141..2181
                     /label=BBa_B0012
     stem_loop       2202..2224
                     /label=stem_loop
     misc_feature    2190..2235
                     /label=BBa_B0011
     terminator      2190..2235
                     /label=BBa_B0011
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggaaa cctactagat ggcagttggc tcgaaaagtt
     1261 ttgcgacagc ctcaaagtta tttgatgcaa aaacccggcg cagcgtactg atgctctacg
     1321 cctggtgccg ccattgtgac gatgttattg acgatcagac gctgggcttt caggcccggc
     1381 agcctgcctt acaaacgccc gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg
     1441 cctatgcagg atcgcagatg cacgaaccgg cgtttgcggc ttttcaggaa gtggctatgg
     1501 ctcatgatat cgccccggct tacgcgtttg atcatctgga aggcttcgcc atggatgtac
     1561 gcgaagcgca atacagccaa ctggatgata cgctgcgcta ttgctatcac gttgcaggcg
     1621 ttgtcggctt gatgatggcg caaatcatgg gcgtacggga taacgccacg ctggaccgcg
     1681 cctgtgacct tgggctggca tttcagttga ccaatattgc tcgcgatatt gtggacgatg
     1741 cgcatgcggg ccgctgttat ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag
     1801 agaattatgc ggcacctgaa aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc
     1861 aggaagcaga accttactat ttgtctgcca cagccggcct ggcagggttg cccctgcgtt
     1921 ccgcctgggc aatcgctacg gcgaagcagg tttaccggaa aataggtgtc aaagttgaac
     1981 aggccggtca gcaagcctgg gatcagcggc agtcaacgac cacgcccgaa aaattaacgc
     2041 tgctgctggc cgcctctggt caggccctta cttcccggat gcgggctcat cctccccgcc
     2101 ctgcgcatct ctggcagcgc ccgctctaat aatactagag tcacactggc tcaccttcgg
     2161 gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg
     2221 gcttttttat tattt
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