##gff-version 3
##sequence-region BBa_J29010 1 3667
BBa_J29010	.	engineered_region	1380	3667	.	+	0	ID=BBa_J29007;Name=BBa_J29007
BBa_J29010	.	promoter	1380	2589	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_J29007
BBa_J29010	.	sequence_feature	1380	2458	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J29010	.	promoter	2422	2493	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J29010	.	promoter	2459	2589	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J29010	.	terminator	3627	3667	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J29007
BBa_J29010	.	engineered_region	3627	3667	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29010	.	stop_codon	3660	3660	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29010	.	stem_loop	3634	3653	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29010	.	polyA_site	3654	3667	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29010	.	terminator	3539	3618	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J29007
BBa_J29010	.	engineered_region	3539	3618	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29010	.	stem_loop	3550	3593	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29010	.	CDS	2619	3530	.	+	0	ID=BBa_J29000;Name=BBa_J29000;Parent=BBa_J29007
BBa_J29010	.	stop_codon	3525	3530	.	+	0	ID=annotation1892587;Name=Double stop codon;Parent=BBa_J29000
BBa_J29010	.	CDS	2619	3530	.	+	0	ID=annotation1892589;Name=CrtE coding sequence;Parent=BBa_J29000
BBa_J29010	.	ribosome_entry_site	2598	2612	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J29007
BBa_J29010	.	engineered_region	2598	2612	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J29010	.	ribosome_entry_site	2605	2609	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J29010	.	ribosome_entry_site	2598	2612	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J29010	.	engineered_region	1	1371	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_J29010	.	terminator	1180	1308	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_J29010	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J29010	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29010	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29010	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J29010	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29010	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29010	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29010	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29010	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_J29010	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J29010	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J29010	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J29010	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J29010	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J29010	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_J29010	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J29010	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J29010	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J29010	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J29010	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_J29010	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J29010
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcacatactagagttatgacaacttgacggctacatcattcactttttcttcacaa
ccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaa
cattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcg
ccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcg
acgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcg
gtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccga
atagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccggg
cgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaaccc
actggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacc
cggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttc
attcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcat
taaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaac
caattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccg
cttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggca
gaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcc
tacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagattaaagagg
agaaatactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatat
tgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctg
gcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggat
tactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgc
gaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctg
agtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaa
acgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagc
tattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcc
tccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccg
atggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggc
ggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccact
caacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata