LOCUS BBa_J29011 3174 bp DNA linear 22-Nov-2024 DEFINITION generates CrtI without arabinose ACCESSION BBa_J29011 VERSION BBa_J29011.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 terminator 1180..1308 /label=BBa_B0015 misc_feature 1319..1335 /label=lac O1 promoter 1336..1341 /label=-35 misc_feature 1342..1358 /label=lac O1 promoter 1359..1364 /label=-10 misc_feature 1317..1370 /label=BBa_R0011 promoter 1317..1370 /label=BBa_R0011 misc_feature 1..1371 /label=BBa_Q04121 misc_feature 1383..1408 /label=c-amp2 misc_feature 1385..1423 /label=araO1 misc_feature 1422..1451 /label=c-amp1 misc_feature 1452..1480 /label=ara1 and ara2 promoter 1492..1497 /label=-35 promoter 1515..1520 /label=-10 promoter 1380..1528 /label=BBa_R0080 RBS 1537..1549 /label=RBS-3\Weak RBS 1543..1546 /label=RBS misc_feature 1537..1549 /label=BBa_B0032 RBS 1537..1549 /label=BBa_B0032 misc_feature 3032..3037 /label=Double stop codon CDS 1556..3037 /label=CrtI coding sequence CDS 1556..3037 /label=BBa_J29002 stem_loop 3057..3100 /label=stem_loop misc_feature 3046..3125 /label=BBa_B0010 terminator 3046..3125 /label=BBa_B0010 stem_loop 3141..3160 /label=T7 TE misc_feature 3167..3167 /label=stop polyA_site 3161..3174 /label=polya misc_feature 3134..3174 /label=BBa_B0012 terminator 3134..3174 /label=BBa_B0012 misc_feature 1380..3174 /label=BBa_J29008 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagagg 1381 cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc 1441 acactttgct atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt 1501 ttatcgcaac tctctactgt ttctccatta ctagagtcac acaggaaagt actagatgaa 1561 accaactacg gtaattggtg caggcttcgg tggcctggca ctggcaattc gtctacaagc 1621 tgcggggatc cccgtcttac tgcttgaaca acgtgataaa cccggcggtc gggcttatgt 1681 ctacgaggat caggggttta cctttgatgc aggcccgacg gttatcaccg atcccagtgc 1741 cattgaagaa ctgtttgcac tggcaggaaa acagttaaaa gagtatgtcg aactgctgcc 1801 ggttacgccg ttttaccgcc tgtgttggga gtcagggaag gtctttaatt acgataacga 1861 tcaaacccgg ctcgaagcgc agattcagca gtttaatccc cgcgatgtcg aaggttatcg 1921 tcagtttctg gactattcac gcgcggtgtt taaagaaggc tatctaaagc tcggtactgt 1981 ccctttttta tcgttcagag acatgcttcg cgccgcacct caactggcga aactccaagc 2041 atggagaagc gtttacagta aggttgccag ttacatcgaa gatgaacatc tgcgccaggc 2101 gttttctttc cactcgctgt tggtgggcgg caatcccttc gccacctcat ccatttatac 2161 gttgatacac gcgctggagc gtgagtgggg cgtctggttt ccgcgtggcg gcaccggcgc 2221 attagttcag gggatgataa agctgtttca ggatctgggt ggcgaagtcg tgttaaacgc 2281 cagagtcagc catatggaaa cgacaggaaa caagattgaa gccgtgcatt tagaggacgg 2341 tcgcaggttc ctgacgcaag ccgtcgcgtc aaatgcagat gtggttcata cctatcgcga 2401 cctgttaagc cagcaccctg ccgcggttaa gcagtccaac aaactccaaa ctaagcgcat 2461 gagtaactct ctgtctgtgc tctattttgg tttgaatcac catcatgatc agctcgcgca 2521 tcacacggtt tgtttcggcc cgcgttaccg cgagctgatt gacgaagttt ttaatcatga 2581 tggcctcgca gaggacttct cactttatct gcacgcgccc tgtgtcacgg attcgtcact 2641 ggcgcctgaa ggttgcggca gttactatgt gttggcgccg gtgccgcatt taggcaccgc 2701 gaacctcgac tggacggttg aggggccaaa actacgcgac cgtatttttg cgtaccttga 2761 gcagcattac atgcctggct tacggagtca gctggtcacg caccggatgt ttacgccgtt 2821 tgattttcgc gaccagctta atgcctatca tggctcagcc ttttctgtgg agcccgttct 2881 tacccagagc gcctggtttc ggccgcataa ccgcgataaa accattacta atctctacct 2941 ggtcggcgca ggcacgcatc ccggcgcagg cattcctggc gtcatcggct cggcaaaagc 3001 gacagcaggt ttgatgctgg aggatctgat ttaataatac tagagccagg catcaaataa 3061 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 3121 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //