##gff-version 3
##sequence-region BBa_J29011 1 3174
BBa_J29011	.	engineered_region	1380	3174	.	+	0	ID=BBa_J29008;Name=BBa_J29008
BBa_J29011	.	CDS	1556	3037	.	+	0	ID=BBa_J29002;Name=BBa_J29002;Parent=BBa_J29008
BBa_J29011	.	CDS	1556	3037	.	+	0	ID=annotation1892591;Name=CrtI coding sequence;Parent=BBa_J29002
BBa_J29011	.	stop_codon	3032	3037	.	+	0	ID=annotation1892588;Name=Double stop codon;Parent=BBa_J29002
BBa_J29011	.	terminator	3134	3174	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J29008
BBa_J29011	.	polyA_site	3161	3174	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29011	.	engineered_region	3134	3174	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29011	.	stem_loop	3141	3160	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29011	.	stop_codon	3167	3167	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29011	.	ribosome_entry_site	1537	1549	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J29008
BBa_J29011	.	engineered_region	1537	1549	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J29011	.	ribosome_entry_site	1537	1549	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J29011	.	ribosome_entry_site	1543	1546	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J29011	.	promoter	1380	1528	.	+	0	ID=BBa_R0080;Name=BBa_R0080;Parent=BBa_J29008
BBa_J29011	.	operator	1385	1423	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_J29011	.	non_covalent_binding_site	1452	1480	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_J29011	.	non_covalent_binding_site	1383	1408	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_J29011	.	promoter	1492	1497	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_J29011	.	non_covalent_binding_site	1422	1451	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_J29011	.	promoter	1515	1520	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_J29011	.	terminator	3046	3125	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J29008
BBa_J29011	.	stem_loop	3057	3100	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29011	.	engineered_region	3046	3125	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29011	.	engineered_region	1	1371	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_J29011	.	terminator	1180	1308	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_J29011	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J29011	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29011	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29011	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29011	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29011	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J29011	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29011	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29011	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_J29011	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J29011	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_J29011	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J29011	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J29011	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J29011	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J29011	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_J29011	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J29011	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J29011	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J29011	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J29011	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
>BBa_J29011
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcacatactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacatt
gattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttt
tatcgcaactctctactgtttctccattactagagtcacacaggaaagtactagatgaaaccaactacggtaattggtg
caggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataa
acccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagt
gccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttacc
gcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaa
tccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggt
actgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactccaagcatggagaagcgttt
acagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaa
tcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggc
accggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagcc
atatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtc
aaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactccaa
actaagcgcatgagtaactctctgtctgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacgg
tttgtttcggcccgcgttaccgcgagctgattgacgaagtttttaatcatgatggcctcgcagaggacttctcacttta
tctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccg
catttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagc
attacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgc
ctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaacc
attactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcga
cagcaggtttgatgctggaggatctgatttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata