LOCUS BBa_J29013 3902 bp DNA linear 22-Nov-2024 DEFINITION this is test2 ACCESSION BBa_J29013 VERSION BBa_J29013.1 FEATURES Location/Qualifiers CDS 1..720 /label=GFP protein CDS 1..720 /label=BBa_E0040 misc_feature 733..736 /label=conserved RBS 729..740 /label=BBa_B0034 misc_feature 1836..1874 /label=LVA CDS 747..1874 /label=lacI-LVA misc_feature 747..1874 /label=BBa_C0012 misc_feature 1875..1899 /label=Help:Barcodes CDS 747..1874 /label=BBa_C0012 stem_loop 1919..1962 /label=stem_loop misc_feature 1908..1987 /label=BBa_B0010 terminator 1908..1987 /label=BBa_B0010 stem_loop 2003..2022 /label=T7 TE misc_feature 2029..2029 /label=stop polyA_site 2023..2036 /label=polya misc_feature 1996..2036 /label=BBa_B0012 terminator 1996..2036 /label=BBa_B0012 misc_feature 2047..2063 /label=lac O1 promoter 2064..2069 /label=-35 misc_feature 2070..2086 /label=lac O1 promoter 2087..2092 /label=-10 misc_feature 2045..2098 /label=BBa_R0011 promoter 2045..2098 /label=BBa_R0011 misc_feature 2111..2136 /label=c-amp2 misc_feature 2113..2151 /label=araO1 misc_feature 2150..2179 /label=c-amp1 misc_feature 2180..2208 /label=ara1 and ara2 promoter 2220..2225 /label=-35 promoter 2243..2248 /label=-10 promoter 2108..2256 /label=BBa_R0080 RBS 2265..2277 /label=RBS-3\Weak RBS 2271..2274 /label=RBS misc_feature 2265..2277 /label=BBa_B0032 RBS 2265..2277 /label=BBa_B0032 misc_feature 3760..3765 /label=Double stop codon CDS 2284..3765 /label=CrtI coding sequence CDS 2284..3765 /label=BBa_J29002 stem_loop 3785..3828 /label=stem_loop misc_feature 3774..3853 /label=BBa_B0010 terminator 3774..3853 /label=BBa_B0010 stem_loop 3869..3888 /label=T7 TE misc_feature 3895..3895 /label=stop polyA_site 3889..3902 /label=polya misc_feature 3862..3902 /label=BBa_B0012 terminator 3862..3902 /label=BBa_B0012 ORIGIN 1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga 121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag 241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc 301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt 361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa 421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga 481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac 541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt 661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa 721 tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg ttatacgatg 781 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc 841 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 901 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 961 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 1021 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 1081 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 1141 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 1201 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 1261 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 1321 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 1381 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 1441 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 1501 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct 1561 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 1621 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 1681 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 1741 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 1801 tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt 1861 tagtagctta ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat 1921 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1981 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2041 agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata ctgagcacat 2101 actagaggcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc 2161 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct 2221 gacgcttttt atcgcaactc tctactgttt ctccattact agagtcacac aggaaagtac 2281 tagatgaaac caactacggt aattggtgca ggcttcggtg gcctggcact ggcaattcgt 2341 ctacaagctg cggggatccc cgtcttactg cttgaacaac gtgataaacc cggcggtcgg 2401 gcttatgtct acgaggatca ggggtttacc tttgatgcag gcccgacggt tatcaccgat 2461 cccagtgcca ttgaagaact gtttgcactg gcaggaaaac agttaaaaga gtatgtcgaa 2521 ctgctgccgg ttacgccgtt ttaccgcctg tgttgggagt cagggaaggt ctttaattac 2581 gataacgatc aaacccggct cgaagcgcag attcagcagt ttaatccccg cgatgtcgaa 2641 ggttatcgtc agtttctgga ctattcacgc gcggtgttta aagaaggcta tctaaagctc 2701 ggtactgtcc cttttttatc gttcagagac atgcttcgcg ccgcacctca actggcgaaa 2761 ctccaagcat ggagaagcgt ttacagtaag gttgccagtt acatcgaaga tgaacatctg 2821 cgccaggcgt tttctttcca ctcgctgttg gtgggcggca atcccttcgc cacctcatcc 2881 atttatacgt tgatacacgc gctggagcgt gagtggggcg tctggtttcc gcgtggcggc 2941 accggcgcat tagttcaggg gatgataaag ctgtttcagg atctgggtgg cgaagtcgtg 3001 ttaaacgcca gagtcagcca tatggaaacg acaggaaaca agattgaagc cgtgcattta 3061 gaggacggtc gcaggttcct gacgcaagcc gtcgcgtcaa atgcagatgt ggttcatacc 3121 tatcgcgacc tgttaagcca gcaccctgcc gcggttaagc agtccaacaa actccaaact 3181 aagcgcatga gtaactctct gtctgtgctc tattttggtt tgaatcacca tcatgatcag 3241 ctcgcgcatc acacggtttg tttcggcccg cgttaccgcg agctgattga cgaagttttt 3301 aatcatgatg gcctcgcaga ggacttctca ctttatctgc acgcgccctg tgtcacggat 3361 tcgtcactgg cgcctgaagg ttgcggcagt tactatgtgt tggcgccggt gccgcattta 3421 ggcaccgcga acctcgactg gacggttgag gggccaaaac tacgcgaccg tatttttgcg 3481 taccttgagc agcattacat gcctggctta cggagtcagc tggtcacgca ccggatgttt 3541 acgccgtttg attttcgcga ccagcttaat gcctatcatg gctcagcctt ttctgtggag 3601 cccgttctta cccagagcgc ctggtttcgg ccgcataacc gcgataaaac cattactaat 3661 ctctacctgg tcggcgcagg cacgcatccc ggcgcaggca ttcctggcgt catcggctcg 3721 gcaaaagcga cagcaggttt gatgctggag gatctgattt aataatacta gagccaggca 3781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3901 ta //