LOCUS BBa_J29015 4049 bp DNA linear 22-Dec-2024 DEFINITION arabinose induable CrtE CrtB CrtI generator ACCESSION BBa_J29015 VERSION BBa_J29015.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1233 /label=RBS-1\Strong RBS 1226..1230 /label=RBS misc_feature 1219..1233 /label=BBa_B0030 RBS 1219..1233 /label=BBa_B0030 stem_loop 1261..1304 /label=stem_loop misc_feature 1250..1329 /label=BBa_B0010 terminator 1250..1329 /label=BBa_B0010 stem_loop 1345..1364 /label=T7 TE misc_feature 1371..1371 /label=stop polyA_site 1365..1378 /label=polya misc_feature 1338..1378 /label=BBa_B0012 terminator 1338..1378 /label=BBa_B0012 terminator 1250..1378 /label=BBa_B0015 misc_feature 1219..1378 /label=BBa_J29003 misc_feature 1393..1396 /label=conserved RBS 1387..1400 /label=BBa_B0031 misc_feature 2295..2300 /label=double stop codon CDS 1407..2300 /label=crtB coding sequence CDS 1407..2300 /label=BBa_J29001 stem_loop 2316..2335 /label=T7 TE misc_feature 2342..2342 /label=stop polyA_site 2336..2349 /label=polya misc_feature 2309..2349 /label=BBa_B0012 terminator 2309..2349 /label=BBa_B0012 stem_loop 2370..2392 /label=stem_loop misc_feature 2358..2403 /label=BBa_B0011 terminator 2358..2403 /label=BBa_B0011 misc_feature 1387..2403 /label=BBa_J29004 RBS 2412..2424 /label=RBS-3\Weak RBS 2418..2421 /label=RBS misc_feature 2412..2424 /label=BBa_B0032 RBS 2412..2424 /label=BBa_B0032 misc_feature 3907..3912 /label=Double stop codon CDS 2431..3912 /label=CrtI coding sequence CDS 2431..3912 /label=BBa_J29002 stem_loop 3932..3975 /label=stem_loop misc_feature 3921..4000 /label=BBa_B0010 terminator 3921..4000 /label=BBa_B0010 stem_loop 4016..4035 /label=T7 TE misc_feature 4042..4042 /label=stop polyA_site 4036..4049 /label=polya misc_feature 4009..4049 /label=BBa_B0012 terminator 4009..4049 /label=BBa_B0012 misc_feature 2412..4049 /label=BBa_J29005 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagat taaagaggag aaatactaga gtactagagc caggcatcaa 1261 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1321 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 1381 ctagagtcac acaggaaacc tactagatgg cagttggctc gaaaagtttt gcgacagcct 1441 caaagttatt tgatgcaaaa acccggcgca gcgtactgat gctctacgcc tggtgccgcc 1501 attgtgacga tgttattgac gatcagacgc tgggctttca ggcccggcag cctgccttac 1561 aaacgcccga acaacgtctg atgcaacttg agatgaaaac gcgccaggcc tatgcaggat 1621 cgcagatgca cgaaccggcg tttgcggctt ttcaggaagt ggctatggct catgatatcg 1681 ccccggctta cgcgtttgat catctggaag gcttcgccat ggatgtacgc gaagcgcaat 1741 acagccaact ggatgatacg ctgcgctatt gctatcacgt tgcaggcgtt gtcggcttga 1801 tgatggcgca aatcatgggc gtacgggata acgccacgct ggaccgcgcc tgtgaccttg 1861 ggctggcatt tcagttgacc aatattgctc gcgatattgt ggacgatgcg catgcgggcc 1921 gctgttatct gccggcaagc tggctggagc atgaaggtct gaacaaagag aattatgcgg 1981 cacctgaaaa ccgtcaggcg ctgagccgta tcgcccgtcg tttggtgcag gaagcagaac 2041 cttactattt gtctgccaca gccggcctgg cagggttgcc cctgcgttcc gcctgggcaa 2101 tcgctacggc gaagcaggtt taccggaaaa taggtgtcaa agttgaacag gccggtcagc 2161 aagcctggga tcagcggcag tcaacgacca cgcccgaaaa attaacgctg ctgctggccg 2221 cctctggtca ggcccttact tcccggatgc gggctcatcc tccccgccct gcgcatctct 2281 ggcagcgccc gctctaataa tactagagtc acactggctc accttcgggt gggcctttct 2341 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta 2401 ttttactaga gtcacacagg aaagtactag atgaaaccaa ctacggtaat tggtgcaggc 2461 ttcggtggcc tggcactggc aattcgtcta caagctgcgg ggatccccgt cttactgctt 2521 gaacaacgtg ataaacccgg cggtcgggct tatgtctacg aggatcaggg gtttaccttt 2581 gatgcaggcc cgacggttat caccgatccc agtgccattg aagaactgtt tgcactggca 2641 ggaaaacagt taaaagagta tgtcgaactg ctgccggtta cgccgtttta ccgcctgtgt 2701 tgggagtcag ggaaggtctt taattacgat aacgatcaaa cccggctcga agcgcagatt 2761 cagcagttta atccccgcga tgtcgaaggt tatcgtcagt ttctggacta ttcacgcgcg 2821 gtgtttaaag aaggctatct aaagctcggt actgtccctt ttttatcgtt cagagacatg 2881 cttcgcgccg cacctcaact ggcgaaactc caagcatgga gaagcgttta cagtaaggtt 2941 gccagttaca tcgaagatga acatctgcgc caggcgtttt ctttccactc gctgttggtg 3001 ggcggcaatc ccttcgccac ctcatccatt tatacgttga tacacgcgct ggagcgtgag 3061 tggggcgtct ggtttccgcg tggcggcacc ggcgcattag ttcaggggat gataaagctg 3121 tttcaggatc tgggtggcga agtcgtgtta aacgccagag tcagccatat ggaaacgaca 3181 ggaaacaaga ttgaagccgt gcatttagag gacggtcgca ggttcctgac gcaagccgtc 3241 gcgtcaaatg cagatgtggt tcatacctat cgcgacctgt taagccagca ccctgccgcg 3301 gttaagcagt ccaacaaact ccaaactaag cgcatgagta actctctgtc tgtgctctat 3361 tttggtttga atcaccatca tgatcagctc gcgcatcaca cggtttgttt cggcccgcgt 3421 taccgcgagc tgattgacga agtttttaat catgatggcc tcgcagagga cttctcactt 3481 tatctgcacg cgccctgtgt cacggattcg tcactggcgc ctgaaggttg cggcagttac 3541 tatgtgttgg cgccggtgcc gcatttaggc accgcgaacc tcgactggac ggttgagggg 3601 ccaaaactac gcgaccgtat ttttgcgtac cttgagcagc attacatgcc tggcttacgg 3661 agtcagctgg tcacgcaccg gatgtttacg ccgtttgatt ttcgcgacca gcttaatgcc 3721 tatcatggct cagccttttc tgtggagccc gttcttaccc agagcgcctg gtttcggccg 3781 cataaccgcg ataaaaccat tactaatctc tacctggtcg gcgcaggcac gcatcccggc 3841 gcaggcattc ctggcgtcat cggctcggca aaagcgacag caggtttgat gctggaggat 3901 ctgatttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 3961 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 4021 accttcgggt gggcctttct gcgtttata //