##gff-version 3
##sequence-region BBa_J29017 1 3290
BBa_J29017	.	CDS	135	1262	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J29017	.	engineered_region	135	1262	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J29017	.	engineered_tag	1263	1287	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J29017	.	tag	1224	1262	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J29017	.	CDS	135	1262	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J29017	.	terminator	3162	3241	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J29017	.	stem_loop	3173	3216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29017	.	engineered_region	3162	3241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29017	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J29017	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J29017	.	terminator	1384	1424	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J29017	.	polyA_site	1411	1424	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29017	.	engineered_region	1384	1424	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29017	.	stem_loop	1391	1410	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29017	.	stop_codon	1417	1417	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29017	.	promoter	1433	1486	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J29017	.	promoter	1452	1457	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J29017	.	promoter	1475	1480	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J29017	.	engineered_region	1433	1486	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J29017	.	operator	1458	1474	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J29017	.	operator	1435	1451	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J29017	.	CDS	1672	3153	.	+	0	ID=BBa_J29002;Name=BBa_J29002
BBa_J29017	.	CDS	1672	3153	.	+	0	ID=annotation1892591;Name=CrtI coding sequence;Parent=BBa_J29002
BBa_J29017	.	stop_codon	3148	3153	.	+	0	ID=annotation1892588;Name=Double stop codon;Parent=BBa_J29002
BBa_J29017	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_J29017	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J29017	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J29017	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J29017	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J29017	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J29017	.	promoter	1496	1644	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_J29017	.	operator	1501	1539	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_J29017	.	non_covalent_binding_site	1568	1596	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_J29017	.	non_covalent_binding_site	1499	1524	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_J29017	.	promoter	1608	1613	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_J29017	.	non_covalent_binding_site	1538	1567	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_J29017	.	promoter	1631	1636	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_J29017	.	terminator	1296	1375	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J29017	.	stem_loop	1307	1350	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29017	.	engineered_region	1296	1375	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29017	.	terminator	3250	3290	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J29017	.	polyA_site	3277	3290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29017	.	engineered_region	3250	3290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29017	.	stem_loop	3257	3276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29017	.	stop_codon	3283	3283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29017	.	ribosome_entry_site	1653	1665	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J29017	.	engineered_region	1653	1665	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J29017	.	ribosome_entry_site	1653	1665	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J29017	.	ribosome_entry_site	1659	1662	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_J29017
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacg
ttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctg
cgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcggg
caaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaa
tctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcgg
tgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtgga
agctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccat
gaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaa
gttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg
ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatg
ctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatct
cggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcct
gctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtc
tcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc
agctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataact
ctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggcgtaa
caaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcattt
ttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagtcacaca
ggaaagtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctg
cggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttac
ctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaa
gagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacg
atcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattc
acgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgca
cctcaactggcgaaactccaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgcc
aggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgct
ggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggat
ctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttag
aggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagcca
gcaccctgccgcggttaagcagtccaacaaactccaaactaagcgcatgagtaactctctgtctgtgctctattttggt
ttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaagttt
ttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctga
aggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggcca
aaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccgga
tgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttaccca
gagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggc
gcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatttaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata