LOCUS BBa_J29018 4210 bp DNA linear 22-Dec-2024 DEFINITION LacI induable lyccopene ACCESSION BBa_J29018 VERSION BBa_J29018.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 terminator 1180..1308 /label=BBa_B0015 misc_feature 1319..1335 /label=lac O1 promoter 1336..1341 /label=-35 misc_feature 1342..1358 /label=lac O1 promoter 1359..1364 /label=-10 misc_feature 1317..1370 /label=BBa_R0011 promoter 1317..1370 /label=BBa_R0011 misc_feature 1..1371 /label=BBa_Q04121 RBS 1380..1394 /label=RBS-1\Strong RBS 1387..1391 /label=RBS misc_feature 1380..1394 /label=BBa_B0030 RBS 1380..1394 /label=BBa_B0030 stem_loop 1422..1465 /label=stem_loop misc_feature 1411..1490 /label=BBa_B0010 terminator 1411..1490 /label=BBa_B0010 stem_loop 1506..1525 /label=T7 TE misc_feature 1532..1532 /label=stop polyA_site 1526..1539 /label=polya misc_feature 1499..1539 /label=BBa_B0012 terminator 1499..1539 /label=BBa_B0012 terminator 1411..1539 /label=BBa_B0015 misc_feature 1380..1539 /label=BBa_J29003 misc_feature 1554..1557 /label=conserved RBS 1548..1561 /label=BBa_B0031 misc_feature 2456..2461 /label=double stop codon CDS 1568..2461 /label=crtB coding sequence CDS 1568..2461 /label=BBa_J29001 stem_loop 2477..2496 /label=T7 TE misc_feature 2503..2503 /label=stop polyA_site 2497..2510 /label=polya misc_feature 2470..2510 /label=BBa_B0012 terminator 2470..2510 /label=BBa_B0012 stem_loop 2531..2553 /label=stem_loop misc_feature 2519..2564 /label=BBa_B0011 terminator 2519..2564 /label=BBa_B0011 misc_feature 1548..2564 /label=BBa_J29004 RBS 2573..2585 /label=RBS-3\Weak RBS 2579..2582 /label=RBS misc_feature 2573..2585 /label=BBa_B0032 RBS 2573..2585 /label=BBa_B0032 misc_feature 4068..4073 /label=Double stop codon CDS 2592..4073 /label=CrtI coding sequence CDS 2592..4073 /label=BBa_J29002 stem_loop 4093..4136 /label=stem_loop misc_feature 4082..4161 /label=BBa_B0010 terminator 4082..4161 /label=BBa_B0010 stem_loop 4177..4196 /label=T7 TE misc_feature 4203..4203 /label=stop polyA_site 4197..4210 /label=polya misc_feature 4170..4210 /label=BBa_B0012 terminator 4170..4210 /label=BBa_B0012 misc_feature 2573..4210 /label=BBa_J29005 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga 1381 ttaaagagga gaaatactag agtactagag ccaggcatca aataaaacga aaggctcagt 1441 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1501 acactggctc accttcgggt gggcctttct gcgtttatat actagagtca cacaggaaac 1561 ctactagatg gcagttggct cgaaaagttt tgcgacagcc tcaaagttat ttgatgcaaa 1621 aacccggcgc agcgtactga tgctctacgc ctggtgccgc cattgtgacg atgttattga 1681 cgatcagacg ctgggctttc aggcccggca gcctgcctta caaacgcccg aacaacgtct 1741 gatgcaactt gagatgaaaa cgcgccaggc ctatgcagga tcgcagatgc acgaaccggc 1801 gtttgcggct tttcaggaag tggctatggc tcatgatatc gccccggctt acgcgtttga 1861 tcatctggaa ggcttcgcca tggatgtacg cgaagcgcaa tacagccaac tggatgatac 1921 gctgcgctat tgctatcacg ttgcaggcgt tgtcggcttg atgatggcgc aaatcatggg 1981 cgtacgggat aacgccacgc tggaccgcgc ctgtgacctt gggctggcat ttcagttgac 2041 caatattgct cgcgatattg tggacgatgc gcatgcgggc cgctgttatc tgccggcaag 2101 ctggctggag catgaaggtc tgaacaaaga gaattatgcg gcacctgaaa accgtcaggc 2161 gctgagccgt atcgcccgtc gtttggtgca ggaagcagaa ccttactatt tgtctgccac 2221 agccggcctg gcagggttgc ccctgcgttc cgcctgggca atcgctacgg cgaagcaggt 2281 ttaccggaaa ataggtgtca aagttgaaca ggccggtcag caagcctggg atcagcggca 2341 gtcaacgacc acgcccgaaa aattaacgct gctgctggcc gcctctggtc aggcccttac 2401 ttcccggatg cgggctcatc ctccccgccc tgcgcatctc tggcagcgcc cgctctaata 2461 atactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag 2521 agaatataaa aagccagatt attaatccgg cttttttatt attttactag agtcacacag 2581 gaaagtacta gatgaaacca actacggtaa ttggtgcagg cttcggtggc ctggcactgg 2641 caattcgtct acaagctgcg gggatccccg tcttactgct tgaacaacgt gataaacccg 2701 gcggtcgggc ttatgtctac gaggatcagg ggtttacctt tgatgcaggc ccgacggtta 2761 tcaccgatcc cagtgccatt gaagaactgt ttgcactggc aggaaaacag ttaaaagagt 2821 atgtcgaact gctgccggtt acgccgtttt accgcctgtg ttgggagtca gggaaggtct 2881 ttaattacga taacgatcaa acccggctcg aagcgcagat tcagcagttt aatccccgcg 2941 atgtcgaagg ttatcgtcag tttctggact attcacgcgc ggtgtttaaa gaaggctatc 3001 taaagctcgg tactgtccct tttttatcgt tcagagacat gcttcgcgcc gcacctcaac 3061 tggcgaaact ccaagcatgg agaagcgttt acagtaaggt tgccagttac atcgaagatg 3121 aacatctgcg ccaggcgttt tctttccact cgctgttggt gggcggcaat cccttcgcca 3181 cctcatccat ttatacgttg atacacgcgc tggagcgtga gtggggcgtc tggtttccgc 3241 gtggcggcac cggcgcatta gttcagggga tgataaagct gtttcaggat ctgggtggcg 3301 aagtcgtgtt aaacgccaga gtcagccata tggaaacgac aggaaacaag attgaagccg 3361 tgcatttaga ggacggtcgc aggttcctga cgcaagccgt cgcgtcaaat gcagatgtgg 3421 ttcataccta tcgcgacctg ttaagccagc accctgccgc ggttaagcag tccaacaaac 3481 tccaaactaa gcgcatgagt aactctctgt ctgtgctcta ttttggtttg aatcaccatc 3541 atgatcagct cgcgcatcac acggtttgtt tcggcccgcg ttaccgcgag ctgattgacg 3601 aagtttttaa tcatgatggc ctcgcagagg acttctcact ttatctgcac gcgccctgtg 3661 tcacggattc gtcactggcg cctgaaggtt gcggcagtta ctatgtgttg gcgccggtgc 3721 cgcatttagg caccgcgaac ctcgactgga cggttgaggg gccaaaacta cgcgaccgta 3781 tttttgcgta ccttgagcag cattacatgc ctggcttacg gagtcagctg gtcacgcacc 3841 ggatgtttac gccgtttgat tttcgcgacc agcttaatgc ctatcatggc tcagcctttt 3901 ctgtggagcc cgttcttacc cagagcgcct ggtttcggcc gcataaccgc gataaaacca 3961 ttactaatct ctacctggtc ggcgcaggca cgcatcccgg cgcaggcatt cctggcgtca 4021 tcggctcggc aaaagcgaca gcaggtttga tgctggagga tctgatttaa taatactaga 4081 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 4141 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 4201 tgcgtttata //