LOCUS BBa_J29019 7099 bp DNA linear 25-Nov-2024 DEFINITION light sensing crtI generator ACCESSION BBa_J29019 VERSION BBa_J29019.1 FEATURES Location/Qualifiers misc_feature 727..751 /label=Help:Barcodes CDS 1..751 /label=BBa_I15008 CDS 758..1507 /label=PcyA misc_feature 1508..1532 /label=Help:Barcodes CDS 758..1532 /label=BBa_I15009 CDS 1539..3776 /label=Cph8 misc_feature 3777..3801 /label=Help:Barcodes CDS 1539..3801 /label=BBa_I15010 misc_feature 3822..3839 /label=C1 OmpR misc_feature 3843..3860 /label=C2 OmpR misc_feature 3863..3880 /label=C3 OmpR promoter 3884..3889 /label=-35 promoter 3907..3912 /label=-10 promoter 3810..3917 /label=BBa_R0082 misc_feature 3930..3933 /label=conserved RBS 3926..3937 /label=BBa_B0034 misc_feature 5033..5071 /label=LVA CDS 3944..5071 /label=lacI-LVA misc_feature 3944..5071 /label=BBa_C0012 misc_feature 5072..5096 /label=Help:Barcodes CDS 3944..5071 /label=BBa_C0012 stem_loop 5116..5159 /label=stem_loop misc_feature 5105..5184 /label=BBa_B0010 terminator 5105..5184 /label=BBa_B0010 stem_loop 5200..5219 /label=T7 TE misc_feature 5226..5226 /label=stop polyA_site 5220..5233 /label=polya misc_feature 5193..5233 /label=BBa_B0012 terminator 5193..5233 /label=BBa_B0012 terminator 5105..5233 /label=BBa_B0015 misc_feature 5244..5260 /label=lac O1 promoter 5261..5266 /label=-35 misc_feature 5267..5283 /label=lac O1 promoter 5284..5289 /label=-10 misc_feature 5242..5295 /label=BBa_R0011 promoter 5242..5295 /label=BBa_R0011 misc_feature 3926..5296 /label=BBa_Q04121 misc_feature 5308..5333 /label=c-amp2 misc_feature 5310..5348 /label=araO1 misc_feature 5347..5376 /label=c-amp1 misc_feature 5377..5405 /label=ara1 and ara2 promoter 5417..5422 /label=-35 promoter 5440..5445 /label=-10 promoter 5305..5453 /label=BBa_R0080 RBS 5462..5474 /label=RBS-3\Weak RBS 5468..5471 /label=RBS misc_feature 5462..5474 /label=BBa_B0032 RBS 5462..5474 /label=BBa_B0032 misc_feature 6957..6962 /label=Double stop codon CDS 5481..6962 /label=CrtI coding sequence CDS 5481..6962 /label=BBa_J29002 stem_loop 6982..7025 /label=stem_loop misc_feature 6971..7050 /label=BBa_B0010 terminator 6971..7050 /label=BBa_B0010 stem_loop 7066..7085 /label=T7 TE misc_feature 7092..7092 /label=stop polyA_site 7086..7099 /label=polya misc_feature 7059..7099 /label=BBa_B0012 terminator 7059..7099 /label=BBa_B0012 misc_feature 5305..7099 /label=BBa_J29008 misc_feature 3926..7099 /label=BBa_J29011 ORIGIN 1 atgagtgtca acttagcttc ccagttgcgg gaagggacga aaaaatccca ctccatggcg 61 gagaacgtcg gctttgtcaa atgcttcctc aagggcgttg tcgagaaaaa ttcctaccgt 121 aagctggttg gcaatctcta ctttgtctac agtgccatgg aagaggaaat ggcaaaattt 181 aaggaccatc ccatcctcag ccacatttac ttccccgaac tcaaccgcaa acaaagccta 241 gagcaagacc tgcaattcta ttacggctcc aactggcggc aagaagtgaa aatttctgcc 301 gctggccaag cctatgtgga ccgagtccgg caagtggccg ctacggcccc tgaattgttg 361 gtggcccatt cctacacccg ttacctgggg gatctttccg gcggtcaaat tctcaagaaa 421 attgcccaaa atgccatgaa tctccacgat ggtggcacag ctttctatga atttgccgac 481 attgatgacg aaaaggcttt taaaaatacc taccgtcaag ctatgaatga tctgcccatt 541 gaccaagcca ccgccgaacg gattgtggat gaagccaatg acgcctttgc catgaacatg 601 aaaatgttca acgaacttga aggcaacctg atcaaggcga tcggcattat ggtgttcaac 661 agcctcaccc gtcgccgcag tcaaggcagc accgaagttg gcctcgccac ctccgaaggc 721 taataacgct gatagtgcta gtgtagatcg ctactagatg gccgtcactg atttaagttt 781 gaccaattct tccctgatgc ctacgttgaa cccgatgatt caacagttgg ccctggcgat 841 cgccgctagt tggcaaagtt tacccctcaa gccctatcaa ttgccggagg atttgggcta 901 cgtagaaggc cgcctggaag gggaaaagtt agtgattgaa aatcggtgct accaaacgcc 961 ccagtttcgc aaaatgcatt tggagttggc caaggtgggc aaagggttgg atattctcca 1021 ctgtgtaatg tttcctgagc ctttatacgg tctacctttg tttggctgtg acattgtggc 1081 cggccccggt ggagtaagtg cggctattgc ggatctatcc cccacccaaa gcgatcgcca 1141 attgcccgca gcgtaccaaa aatcattggc agagctaggc cagccagaat ttgagcaaca 1201 acgggaattg cccccctggg gagaaatatt ttctgaatat tgtttattca tccgtcccag 1261 caatgtcact gaagaagaaa gatttgtaca aagggtagtg gactttttgc aaattcattg 1321 tcaccaatcc atcgttgccg aacccttgtc tgaagctcaa actttggagc accgtcaggg 1381 gcaaattcat tactgccaac aacaacagaa aaatgataaa acccgtcggg tactggaaaa 1441 agcttttggg gaagcttggg cggaacggta tatgagccaa gtcttatttg atgttatcca 1501 ataataactc tgatagtgct agtgtagatc tctactagat ggccaccacc gtacaactca 1561 gcgaccaatc cctccgtcag ctagaaaccc tcgccatcca caccgcccac ctgattcagc 1621 cccacggttt agtggtggtc ctgcaagaac cagacctcac catcagccaa attagcgcca 1681 actgcaccgg cattttaggg cgatcgccag aggatttgtt gggcagaacc ctaggggaag 1741 tgtttgatag ctttcagatt gatcccatcc agagtcgcct aacggccgga caaatcagca 1801 gcctcaaccc cagtaaactt tgggcgcggg tcatggggga cgactttgtc atttttgacg 1861 gggtttttca tcgcaacagt gacggtttat tggtatgtga actcgagcca gcctacactt 1921 ccgataatct gcccttcctc ggtttttatc acatggccaa cgctgccctg aatcggttgc 1981 gccaacaagc taatctacgg gatttctacg atgttattgt cgaagaagtc cgccgtatga 2041 ctggctttga ccgggtgatg ctataccgct ttgatgaaaa taaccacggt gatgtcattg 2101 ccgaagataa acgggatgat atggaaccct atttgggcct gcactatccc gaatcggata 2161 ttccccaacc cgcccgtcgg ctatttatcc acaaccccat tcgagtaatt cccgatgttt 2221 atggtgtggc ggtgcccctg accccagcgg ttaaccccag caccaaccga gcggtggatt 2281 taacagaatc cattctgcgc agtgcgtacc attgccactt gacctatctg aaaaatatgg 2341 gggtaggagc gtctttaacc atttccctaa ttaaggacgg ccatctctgg gggctcattg 2401 cctgccacca tcaaaccccc aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt 2461 ttggtcgggt ggtgtttagc aacatttccg cccaggaaga tacggaaacc ttcgattacc 2521 gggtgcagct ggcggagcat gaagcggttt tattggacaa aatgaccacg gcggcggatt 2581 ttgtcgaagg attaactaat catcccgatc gcctgttggg attaacgggc tcccaggggg 2641 cggccatttg ctttggggaa aaattgattt tagtagggga aaccccggac gagaaagcag 2701 tgcaatattt actgcaatgg ttggagaatc gggaagtgca agacgttttc ttcacctctt 2761 ccctctcaca aatttatcct gatgcagtga attttaaatc cgtggccagt ggcttattgg 2821 ccattcccat tgcccgtcac aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg 2881 ttaattgggg cggtgaccca aatcatgctt acgaagctac ccaggaagac ggtaaaatcg 2941 agctccatcc ccgccaatcc tttgacctct ggaaagaaat tgtccgactc caatctttgc 3001 cctggcaatc ggtggaaatc caaagtgccc tggccctgaa aaaggcgatc gtcaacctca 3061 ttttgcgcca ggcagaagaa ttgcatatgg cggctggtgt taagcaactg gcggatgacc 3121 gcacgctgct gatggcgggg gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc 3181 tggcgactga gatgatgagc gagcaggatg gctatctggc agaatcgatc aataaagata 3241 tcgaagagtg caacgccatc attgagcagt ttatcgacta cctgcgcacc gggcaggaga 3301 tgccgatgga aatggcggat cttaatgcag tactcggtga ggtgattgct gccgaaagtg 3361 gctatgagcg ggaaattgaa accgcgcttt accccggcag cattgaagtg aaaatgcacc 3421 cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg 3481 gctgggtcaa agtcagcagc ggaacggagc cgaatcgcgc ctggttccag gtggaagatg 3541 acggtccggg aattgcgccg gaacaacgta agcacctgtt ccagccgttt gtccgcggcg 3601 acagtgcgcg caccattagc ggcacgggat tagggctggc aattgtgcag cgtatcgtgg 3661 ataaccataa cgggatgctg gagcttggca ccagcgagcg gggcgggctt tccattcgcg 3721 cctggctgcc agtgccggta acgcgggcgc agggcatgac aaaagaaggg taataactct 3781 gatagtgcta gtgtagatct ctactagagt cccttgcatt tacattttga aacatctata 3841 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt 3901 tttggggaga atggacttac tagagaaaga ggagaaatac tagatggtga atgtgaaacc 3961 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 4021 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 4081 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 4141 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 4201 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 4261 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 4321 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 4381 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 4441 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 4501 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 4561 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 4621 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 4681 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 4741 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 4801 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 4861 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 4921 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4981 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa 5041 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact 5101 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5161 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 5221 ttctgcgttt atatactaga gaattgtgag cggataacaa ttgacattgt gagcggataa 5281 caagatactg agcacatact agaggcgtaa caaaagtgtc tataatcacg gcagaaaagt 5341 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag 5401 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc cattactaga 5461 gtcacacagg aaagtactag atgaaaccaa ctacggtaat tggtgcaggc ttcggtggcc 5521 tggcactggc aattcgtcta caagctgcgg ggatccccgt cttactgctt gaacaacgtg 5581 ataaacccgg cggtcgggct tatgtctacg aggatcaggg gtttaccttt gatgcaggcc 5641 cgacggttat caccgatccc agtgccattg aagaactgtt tgcactggca ggaaaacagt 5701 taaaagagta tgtcgaactg ctgccggtta cgccgtttta ccgcctgtgt tgggagtcag 5761 ggaaggtctt taattacgat aacgatcaaa cccggctcga agcgcagatt cagcagttta 5821 atccccgcga tgtcgaaggt tatcgtcagt ttctggacta ttcacgcgcg gtgtttaaag 5881 aaggctatct aaagctcggt actgtccctt ttttatcgtt cagagacatg cttcgcgccg 5941 cacctcaact ggcgaaactc caagcatgga gaagcgttta cagtaaggtt gccagttaca 6001 tcgaagatga acatctgcgc caggcgtttt ctttccactc gctgttggtg ggcggcaatc 6061 ccttcgccac ctcatccatt tatacgttga tacacgcgct ggagcgtgag tggggcgtct 6121 ggtttccgcg tggcggcacc ggcgcattag ttcaggggat gataaagctg tttcaggatc 6181 tgggtggcga agtcgtgtta aacgccagag tcagccatat ggaaacgaca ggaaacaaga 6241 ttgaagccgt gcatttagag gacggtcgca ggttcctgac gcaagccgtc gcgtcaaatg 6301 cagatgtggt tcatacctat cgcgacctgt taagccagca ccctgccgcg gttaagcagt 6361 ccaacaaact ccaaactaag cgcatgagta actctctgtc tgtgctctat tttggtttga 6421 atcaccatca tgatcagctc gcgcatcaca cggtttgttt cggcccgcgt taccgcgagc 6481 tgattgacga agtttttaat catgatggcc tcgcagagga cttctcactt tatctgcacg 6541 cgccctgtgt cacggattcg tcactggcgc ctgaaggttg cggcagttac tatgtgttgg 6601 cgccggtgcc gcatttaggc accgcgaacc tcgactggac ggttgagggg ccaaaactac 6661 gcgaccgtat ttttgcgtac cttgagcagc attacatgcc tggcttacgg agtcagctgg 6721 tcacgcaccg gatgtttacg ccgtttgatt ttcgcgacca gcttaatgcc tatcatggct 6781 cagccttttc tgtggagccc gttcttaccc agagcgcctg gtttcggccg cataaccgcg 6841 ataaaaccat tactaatctc tacctggtcg gcgcaggcac gcatcccggc gcaggcattc 6901 ctggcgtcat cggctcggca aaagcgacag caggtttgat gctggaggat ctgatttaat 6961 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 7021 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 7081 gggcctttct gcgtttata //