##gff-version 3
##sequence-region BBa_J29019 1 7099
BBa_J29019	.	CDS	1	751	.	+	0	ID=BBa_I15008;Name=BBa_I15008
BBa_J29019	.	engineered_tag	727	751	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_J29019	.	CDS	1539	3801	.	+	0	ID=BBa_I15010;Name=BBa_I15010
BBa_J29019	.	engineered_tag	3777	3801	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_J29019	.	CDS	1539	3776	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_J29019	.	CDS	758	1532	.	+	0	ID=BBa_I15009;Name=BBa_I15009
BBa_J29019	.	CDS	758	1507	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_J29019	.	engineered_tag	1508	1532	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_J29019	.	promoter	3810	3917	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_J29019	.	non_covalent_binding_site	3863	3880	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J29019	.	promoter	3907	3912	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J29019	.	promoter	3884	3889	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J29019	.	non_covalent_binding_site	3822	3839	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J29019	.	non_covalent_binding_site	3843	3860	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J29019	.	engineered_region	3926	7099	.	+	0	ID=BBa_J29011;Name=BBa_J29011
BBa_J29019	.	engineered_region	3926	5296	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_J29011
BBa_J29019	.	ribosome_entry_site	3926	3937	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_J29019	.	conserved_region	3930	3933	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J29019	.	promoter	5242	5295	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_J29019	.	promoter	5284	5289	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J29019	.	operator	5244	5260	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J29019	.	operator	5267	5283	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J29019	.	engineered_region	5242	5295	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J29019	.	promoter	5261	5266	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J29019	.	terminator	5105	5233	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_J29019	.	terminator	5105	5184	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J29019	.	stem_loop	5116	5159	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29019	.	engineered_region	5105	5184	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29019	.	terminator	5193	5233	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J29019	.	stop_codon	5226	5226	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29019	.	engineered_region	5193	5233	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29019	.	stem_loop	5200	5219	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29019	.	polyA_site	5220	5233	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29019	.	CDS	3944	5071	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_J29019	.	engineered_region	3944	5071	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J29019	.	engineered_tag	5072	5096	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J29019	.	CDS	3944	5071	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J29019	.	tag	5033	5071	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J29019	.	engineered_region	5305	7099	.	+	0	ID=BBa_J29008;Name=BBa_J29008;Parent=BBa_J29011
BBa_J29019	.	CDS	5481	6962	.	+	0	ID=BBa_J29002;Name=BBa_J29002;Parent=BBa_J29008
BBa_J29019	.	stop_codon	6957	6962	.	+	0	ID=annotation1892588;Name=Double stop codon;Parent=BBa_J29002
BBa_J29019	.	CDS	5481	6962	.	+	0	ID=annotation1892591;Name=CrtI coding sequence;Parent=BBa_J29002
BBa_J29019	.	terminator	7059	7099	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J29008
BBa_J29019	.	stop_codon	7092	7092	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29019	.	engineered_region	7059	7099	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29019	.	stem_loop	7066	7085	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29019	.	polyA_site	7086	7099	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29019	.	promoter	5305	5453	.	+	0	ID=BBa_R0080;Name=BBa_R0080;Parent=BBa_J29008
BBa_J29019	.	non_covalent_binding_site	5347	5376	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_J29019	.	non_covalent_binding_site	5377	5405	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_J29019	.	promoter	5417	5422	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_J29019	.	promoter	5440	5445	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_J29019	.	non_covalent_binding_site	5308	5333	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_J29019	.	operator	5310	5348	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_J29019	.	terminator	6971	7050	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J29008
BBa_J29019	.	stem_loop	6982	7025	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29019	.	engineered_region	6971	7050	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29019	.	ribosome_entry_site	5462	5474	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J29008
BBa_J29019	.	engineered_region	5462	5474	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J29019	.	ribosome_entry_site	5462	5474	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J29019	.	ribosome_entry_site	5468	5471	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_J29019
atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtca
aatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccat
ggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagc
ctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatg
tggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatct
ttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaattt
gccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccg
ccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgat
caaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacc
tccgaaggctaataacgctgatagtgctagtgtagatcgctactagatggccgtcactgatttaagtttgaccaattct
tccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctca
agccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtg
ctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgta
atgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggcta
ttgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagcc
agaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaat
gtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaac
ccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaac
ccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaa
taataactctgatagtgctagtgtagatctctactagatggccaccaccgtacaactcagcgaccaatccctccgtcag
ctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctca
ccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctagggga
agtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaa
ctttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtat
gtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcg
gttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgg
gtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatt
tgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcc
cgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatcc
attctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaa
ttaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagc
ctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcag
ctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccg
atcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaacccc
ggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctc
tcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaact
ttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctaccca
ggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccc
tggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaat
tgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcac
gccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagat
atcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcgg
atcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccgg
cagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggc
aatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattg
cgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagg
gctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttcc
attcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagt
gtagatctctactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagt
atcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtga
atgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca
ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg
gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa
ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga
agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag
gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca
gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct
gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt
cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca
tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg
cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc
aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca
atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt
ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagc
acatactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacacttt
gctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctcc
attactagagtcacacaggaaagtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggc
aattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctac
gaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactgg
caggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggt
ctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgt
cagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagag
acatgcttcgcgccgcacctcaactggcgaaactccaagcatggagaagcgtttacagtaaggttgccagttacatcga
agatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttat
acgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatga
taaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagat
tgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctat
cgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactccaaactaagcgcatgagtaactctctgt
ctgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcga
gctgattgacgaagtttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggat
tcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgact
ggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtca
gctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtg
gagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcg
caggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatct
gatttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata