LOCUS BBa_J29020 5795 bp DNA linear 22-Nov-2024 DEFINITION light sencer + lycopene ACCESSION BBa_J29020 VERSION BBa_J29020.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 misc_feature 61..972 /label=BBa_J29000 CDS 61..972 /label=CrtE coding sequence misc_feature 967..972 /label=Double stop codon misc_feature 979..1872 /label=BBa_J29001 CDS 979..1872 /label=crtB coding sequence misc_feature 1867..1872 /label=double stop codon misc_feature 1879..2604 /label=BBa_I15008 misc_feature 2605..2629 /label=barcode CDS 2636..3385 /label=PcyA misc_feature 2636..3385 /label=BBa_I15009 misc_feature 3386..3410 /label=barcode misc_feature 3417..5654 /label=BBa_I15010 CDS 3417..5654 /label=Cph8 misc_feature 5655..5679 /label=barcode misc_feature 5688..5795 /label=BBa_R0082 misc_feature 5700..5717 /label=C1 OmpR misc_feature 5721..5738 /label=C2 OmpR misc_feature 5741..5758 /label=C3 OmpR promoter 5762..5767 /label=-35 promoter 5785..5790 /label=-10 misc_feature 1..5795 /label=BBa_J29020 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 atgacggtct gcgcaaaaaa acacgttcat ctcactcgcg atgctgcgga gcagttactg 121 gctgatattg atcgacgcct tgatcagtta ttgcccgtgg agggagaacg ggatgttgtg 181 ggtgccgcga tgcgtgaagg tgcgctggca ccgggaaaac gtattcgccc catgttgctg 241 ttgctgaccg cccgcgatct gggttgcgct gtcagccatg acggattact ggatttggcc 301 tgtgcggtgg aaatggtcca cgcggcttcg ctgatccttg acgatatgcc ctgcatggac 361 gatgcgaagc tgcggcgcgg acgccctacc attcattctc attacggaga gcatgtggca 421 atactggcgg cggttgcctt gctgagtaaa gcctttggcg taattgccga tgcagatggc 481 ctcacgccgc tggcaaaaaa tcgggcggtt tctgaactgt caaacgccat cggcatgcaa 541 ggattggttc agggtcagtt caaggatctg tctgaagggg ataagccgcg cagcgctgaa 601 gctattttga tgacgaatca ctttaaaacc agcacgctgt tttgtgcctc catgcagatg 661 gcctcgattg ttgcgaatgc ctccagcgaa gcgcgtgatt gcctgcatcg tttttcactt 721 gatcttggtc aggcatttca actgctggac gatttgaccg atggcatgac cgacaccggt 781 aaggatagca atcaggacgc cggtaaatcg acgctggtca atctgttagg cccgagggcg 841 gttgaagaac gtctgagaca acatcttcag cttgccagtg agcatctctc tgcggcctgc 901 caacacgggc acgccactca acattttatt caggcctggt ttgacaaaaa actcgctgcc 961 gtcagttaat aatactagat ggcagttggc tcgaaaagtt ttgcgacagc ctcaaagtta 1021 tttgatgcaa aaacccggcg cagcgtactg atgctctacg cctggtgccg ccattgtgac 1081 gatgttattg acgatcagac gctgggcttt caggcccggc agcctgcctt acaaacgccc 1141 gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg cctatgcagg atcgcagatg 1201 cacgaaccgg cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat cgccccggct 1261 tacgcgtttg atcatctgga aggcttcgcc atggatgtac gcgaagcgca atacagccaa 1321 ctggatgata cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt gatgatggcg 1381 caaatcatgg gcgtacggga taacgccacg ctggaccgcg cctgtgacct tgggctggca 1441 tttcagttga ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg ccgctgttat 1501 ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag agaattatgc ggcacctgaa 1561 aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga accttactat 1621 ttgtctgcca cagccggcct ggcagggttg cccctgcgtt ccgcctgggc aatcgctacg 1681 gcgaagcagg tttaccggaa aataggtgtc aaagttgaac aggccggtca gcaagcctgg 1741 gatcagcggc agtcaacgac cacgcccgaa aaattaacgc tgctgctggc cgcctctggt 1801 caggccctta cttcccggat gcgggctcat cctccccgcc ctgcgcatct ctggcagcgc 1861 ccgctctaat aatactagat gagtgtcaac ttagcttccc agttgcggga agggacgaaa 1921 aaatcccact ccatggcgga gaacgtcggc tttgtcaaat gcttcctcaa gggcgttgtc 1981 gagaaaaatt cctaccgtaa gctggttggc aatctctact ttgtctacag tgccatggaa 2041 gaggaaatgg caaaatttaa ggaccatccc atcctcagcc acatttactt ccccgaactc 2101 aaccgcaaac aaagcctaga gcaagacctg caattctatt acggctccaa ctggcggcaa 2161 gaagtgaaaa tttctgccgc tggccaagcc tatgtggacc gagtccggca agtggccgct 2221 acggcccctg aattgttggt ggcccattcc tacacccgtt acctggggga tctttccggc 2281 ggtcaaattc tcaagaaaat tgcccaaaat gccatgaatc tccacgatgg tggcacagct 2341 ttctatgaat ttgccgacat tgatgacgaa aaggctttta aaaataccta ccgtcaagct 2401 atgaatgatc tgcccattga ccaagccacc gccgaacgga ttgtggatga agccaatgac 2461 gcctttgcca tgaacatgaa aatgttcaac gaacttgaag gcaacctgat caaggcgatc 2521 ggcattatgg tgttcaacag cctcacccgt cgccgcagtc aaggcagcac cgaagttggc 2581 ctcgccacct ccgaaggcta ataacgctga tagtgctagt gtagatcgct actagatggc 2641 cgtcactgat ttaagtttga ccaattcttc cctgatgcct acgttgaacc cgatgattca 2701 acagttggcc ctggcgatcg ccgctagttg gcaaagttta cccctcaagc cctatcaatt 2761 gccggaggat ttgggctacg tagaaggccg cctggaaggg gaaaagttag tgattgaaaa 2821 tcggtgctac caaacgcccc agtttcgcaa aatgcatttg gagttggcca aggtgggcaa 2881 agggttggat attctccact gtgtaatgtt tcctgagcct ttatacggtc tacctttgtt 2941 tggctgtgac attgtggccg gccccggtgg agtaagtgcg gctattgcgg atctatcccc 3001 cacccaaagc gatcgccaat tgcccgcagc gtaccaaaaa tcattggcag agctaggcca 3061 gccagaattt gagcaacaac gggaattgcc cccctgggga gaaatatttt ctgaatattg 3121 tttattcatc cgtcccagca atgtcactga agaagaaaga tttgtacaaa gggtagtgga 3181 ctttttgcaa attcattgtc accaatccat cgttgccgaa cccttgtctg aagctcaaac 3241 tttggagcac cgtcaggggc aaattcatta ctgccaacaa caacagaaaa atgataaaac 3301 ccgtcgggta ctggaaaaag cttttgggga agcttgggcg gaacggtata tgagccaagt 3361 cttatttgat gttatccaat aataactctg atagtgctag tgtagatctc tactagatgg 3421 ccaccaccgt acaactcagc gaccaatccc tccgtcagct agaaaccctc gccatccaca 3481 ccgcccacct gattcagccc cacggtttag tggtggtcct gcaagaacca gacctcacca 3541 tcagccaaat tagcgccaac tgcaccggca ttttagggcg atcgccagag gatttgttgg 3601 gcagaaccct aggggaagtg tttgatagct ttcagattga tcccatccag agtcgcctaa 3661 cggccggaca aatcagcagc ctcaacccca gtaaactttg ggcgcgggtc atgggggacg 3721 actttgtcat ttttgacggg gtttttcatc gcaacagtga cggtttattg gtatgtgaac 3781 tcgagccagc ctacacttcc gataatctgc ccttcctcgg tttttatcac atggccaacg 3841 ctgccctgaa tcggttgcgc caacaagcta atctacggga tttctacgat gttattgtcg 3901 aagaagtccg ccgtatgact ggctttgacc gggtgatgct ataccgcttt gatgaaaata 3961 accacggtga tgtcattgcc gaagataaac gggatgatat ggaaccctat ttgggcctgc 4021 actatcccga atcggatatt ccccaacccg cccgtcggct atttatccac aaccccattc 4081 gagtaattcc cgatgtttat ggtgtggcgg tgcccctgac cccagcggtt aaccccagca 4141 ccaaccgagc ggtggattta acagaatcca ttctgcgcag tgcgtaccat tgccacttga 4201 cctatctgaa aaatatgggg gtaggagcgt ctttaaccat ttccctaatt aaggacggcc 4261 atctctgggg gctcattgcc tgccaccatc aaacccccaa agtaattccc tttgaactgc 4321 gtaaagcctg cgaatttttt ggtcgggtgg tgtttagcaa catttccgcc caggaagata 4381 cggaaacctt cgattaccgg gtgcagctgg cggagcatga agcggtttta ttggacaaaa 4441 tgaccacggc ggcggatttt gtcgaaggat taactaatca tcccgatcgc ctgttgggat 4501 taacgggctc ccagggggcg gccatttgct ttggggaaaa attgatttta gtaggggaaa 4561 ccccggacga gaaagcagtg caatatttac tgcaatggtt ggagaatcgg gaagtgcaag 4621 acgttttctt cacctcttcc ctctcacaaa tttatcctga tgcagtgaat tttaaatccg 4681 tggccagtgg cttattggcc attcccattg cccgtcacaa ctttttgctc tggtttcgcc 4741 ctgaagtgtt gcaaacggtt aattggggcg gtgacccaaa tcatgcttac gaagctaccc 4801 aggaagacgg taaaatcgag ctccatcccc gccaatcctt tgacctctgg aaagaaattg 4861 tccgactcca atctttgccc tggcaatcgg tggaaatcca aagtgccctg gccctgaaaa 4921 aggcgatcgt caacctcatt ttgcgccagg cagaagaatt gcatatggcg gctggtgtta 4981 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc 5041 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag 5101 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc 5161 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagta ctcggtgagg 5221 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca 5281 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg 5341 ccgcccgtta tggcaatggc tgggtcaaag tcagcagcgg aacggagccg aatcgcgcct 5401 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc 5461 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa 5521 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg 5581 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcatgacaa 5641 aagaagggta ataactctga tagtgctagt gtagatctct actagagtcc cttgcattta 5701 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt 5761 gttggattat tctgcatttt tggggagaat ggact 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