##gff-version 3
##sequence-region BBa_J29020 1 5795
BBa_J29020	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation2264954;Name=TetR 2
BBa_J29020	.	engineered_region	3417	5654	.	+	0	ID=annotation2264967;Name=BBa_I15010
BBa_J29020	.	engineered_region	2636	3385	.	+	0	ID=annotation2264965;Name=BBa_I15009
BBa_J29020	.	CDS	2636	3385	.	+	0	ID=annotation2264964;Name=PcyA
BBa_J29020	.	CDS	979	1872	.	+	0	ID=annotation2264960;Name=crtB coding sequence
BBa_J29020	.	CDS	61	972	.	+	0	ID=annotation2264957;Name=CrtE coding sequence
BBa_J29020	.	CDS	3417	5654	.	+	0	ID=annotation2264968;Name=Cph8
BBa_J29020	.	promoter	43	48	.	+	0	ID=annotation2264955;Name=-10
BBa_J29020	.	stop_codon	1867	1872	.	+	0	ID=annotation2264961;Name=double stop codon
BBa_J29020	.	stop_codon	967	972	.	+	0	ID=annotation2264958;Name=Double stop codon
BBa_J29020	.	non_covalent_binding_site	5721	5738	.	+	0	ID=annotation2264972;Name=C2 OmpR
BBa_J29020	.	promoter	20	25	.	+	0	ID=annotation2264953;Name=-35
BBa_J29020	.	engineered_region	1879	2604	.	+	0	ID=annotation2264962;Name=BBa_I15008
BBa_J29020	.	promoter	5785	5790	.	+	0	ID=annotation2264975;Name=-10
BBa_J29020	.	engineered_region	979	1872	.	+	0	ID=annotation2264959;Name=BBa_J29001
BBa_J29020	.	engineered_region	2605	2629	.	+	0	ID=annotation2264963;Name=barcode
BBa_J29020	.	engineered_region	3386	3410	.	+	0	ID=annotation2264966;Name=barcode
BBa_J29020	.	engineered_region	61	972	.	+	0	ID=annotation2264956;Name=BBa_J29000
BBa_J29020	.	non_covalent_binding_site	5741	5758	.	+	0	ID=annotation2264973;Name=C3 OmpR
BBa_J29020	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation2264951;Name=TetR 1
BBa_J29020	.	engineered_region	5655	5679	.	+	0	ID=annotation2264969;Name=barcode
BBa_J29020	.	engineered_region	1	54	.	+	0	ID=annotation2264952;Name=BBa_R0040
BBa_J29020	.	promoter	5762	5767	.	+	0	ID=annotation2264974;Name=-35
BBa_J29020	.	non_covalent_binding_site	5700	5717	.	+	0	ID=annotation2264971;Name=C1 OmpR
BBa_J29020	.	engineered_region	1	5795	.	+	0	ID=annotation2264976;Name=BBa_J29020
BBa_J29020	.	engineered_region	5688	5795	.	+	0	ID=annotation2264970;Name=BBa_R0082
>BBa_J29020
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgacggtctgcgcaaaaa
aacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgt
ggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttg
ctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatgg
tccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattca
ttctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgca
gatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagg
gtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccag
cacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgt
ttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagca
atcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttca
gcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaa
aaactcgctgccgtcagttaataatactagatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatg
caaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctggg
ctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctat
gcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacg
cgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattg
ctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcc
tgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatc
tgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccg
tatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgt
tccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcct
gggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccg
gatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagatgagtgtcaacttagct
tcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcg
ttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaa
atttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaa
ttctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaag
tggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattct
caagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaa
aaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatg
aagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattat
ggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgc
tgatagtgctagtgtagatcgctactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttg
aacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccgg
aggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtt
tcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagccttta
tacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatccccca
cccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacg
ggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaaga
tttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaa
ctttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaa
agcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgc
tagtgtagatctctactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatc
cacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcg
ccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttca
gattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatg
ggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcct
acacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaa
tctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgcttt
gatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccg
aatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggc
ggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtac
cattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctct
gggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcg
ggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcg
gttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaa
cgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgca
atatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgat
gcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgcc
ctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcga
gctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatc
caaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtg
ttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcg
cctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgcc
atcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcg
gtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaat
gcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtc
agcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagc
acctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcg
tatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgcca
gtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctctactagagt
cccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggat
tattctgcatttttggggagaatggact