LOCUS BBa_J29021 3638 bp DNA linear 22-Nov-2024 DEFINITION This is 4 enzymes that is used to produce a β-carotene. ACCESSION BBa_J29021 VERSION BBa_J29021.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 RBS 32..46 /label=RBS-1\Strong RBS 39..43 /label=RBS misc_feature 32..46 /label=BBa_B0030 RBS 32..46 /label=BBa_B0030 misc_feature 941..946 /label=double stop codon CDS 53..946 /label=crtB coding sequence CDS 53..946 /label=BBa_J29001 RBS 955..969 /label=RBS-1\Strong RBS 962..966 /label=RBS misc_feature 955..969 /label=BBa_B0030 RBS 955..969 /label=BBa_B0030 misc_feature 2452..2457 /label=Double stop codon CDS 976..2457 /label=CrtI coding sequence CDS 976..2457 /label=BBa_J29002 RBS 2466..2480 /label=RBS-1\Strong RBS 2473..2477 /label=RBS misc_feature 2466..2480 /label=BBa_B0030 RBS 2466..2480 /label=BBa_B0030 CDS 2487..3638 /label=BBa_J29030 ORIGIN 1 attaaagagg agaaatacta gagtactaga gattaaagag gagaaatact agatggcagt 61 tggctcgaaa agttttgcga cagcctcaaa gttatttgat gcaaaaaccc ggcgcagcgt 121 actgatgctc tacgcctggt gccgccattg tgacgatgtt attgacgatc agacgctggg 181 ctttcaggcc cggcagcctg ccttacaaac gcccgaacaa cgtctgatgc aacttgagat 241 gaaaacgcgc caggcctatg caggatcgca gatgcacgaa ccggcgtttg cggcttttca 301 ggaagtggct atggctcatg atatcgcccc ggcttacgcg tttgatcatc tggaaggctt 361 cgccatggat gtacgcgaag cgcaatacag ccaactggat gatacgctgc gctattgcta 421 tcacgttgca ggcgttgtcg gcttgatgat ggcgcaaatc atgggcgtac gggataacgc 481 cacgctggac cgcgcctgtg accttgggct ggcatttcag ttgaccaata ttgctcgcga 541 tattgtggac gatgcgcatg cgggccgctg ttatctgccg gcaagctggc tggagcatga 601 aggtctgaac aaagagaatt atgcggcacc tgaaaaccgt caggcgctga gccgtatcgc 661 ccgtcgtttg gtgcaggaag cagaacctta ctatttgtct gccacagccg gcctggcagg 721 gttgcccctg cgttccgcct gggcaatcgc tacggcgaag caggtttacc ggaaaatagg 781 tgtcaaagtt gaacaggccg gtcagcaagc ctgggatcag cggcagtcaa cgaccacgcc 841 cgaaaaatta acgctgctgc tggccgcctc tggtcaggcc cttacttccc ggatgcgggc 901 tcatcctccc cgccctgcgc atctctggca gcgcccgctc taataatact agagattaaa 961 gaggagaaat actagatgaa accaactacg gtaattggtg caggcttcgg tggcctggca 1021 ctggcaattc gtctacaagc tgcggggatc cccgtcttac tgcttgaaca acgtgataaa 1081 cccggcggtc gggcttatgt ctacgaggat caggggttta cctttgatgc aggcccgacg 1141 gttatcaccg atcccagtgc cattgaagaa ctgtttgcac tggcaggaaa acagttaaaa 1201 gagtatgtcg aactgctgcc ggttacgccg ttttaccgcc tgtgttggga gtcagggaag 1261 gtctttaatt acgataacga tcaaacccgg ctcgaagcgc agattcagca gtttaatccc 1321 cgcgatgtcg aaggttatcg tcagtttctg gactattcac gcgcggtgtt taaagaaggc 1381 tatctaaagc tcggtactgt ccctttttta tcgttcagag acatgcttcg cgccgcacct 1441 caactggcga aactccaagc atggagaagc gtttacagta aggttgccag ttacatcgaa 1501 gatgaacatc tgcgccaggc gttttctttc cactcgctgt tggtgggcgg caatcccttc 1561 gccacctcat ccatttatac gttgatacac gcgctggagc gtgagtgggg cgtctggttt 1621 ccgcgtggcg gcaccggcgc attagttcag gggatgataa agctgtttca ggatctgggt 1681 ggcgaagtcg tgttaaacgc cagagtcagc catatggaaa cgacaggaaa caagattgaa 1741 gccgtgcatt tagaggacgg tcgcaggttc ctgacgcaag ccgtcgcgtc aaatgcagat 1801 gtggttcata cctatcgcga cctgttaagc cagcaccctg ccgcggttaa gcagtccaac 1861 aaactccaaa ctaagcgcat gagtaactct ctgtctgtgc tctattttgg tttgaatcac 1921 catcatgatc agctcgcgca tcacacggtt tgtttcggcc cgcgttaccg cgagctgatt 1981 gacgaagttt ttaatcatga tggcctcgca gaggacttct cactttatct gcacgcgccc 2041 tgtgtcacgg attcgtcact ggcgcctgaa ggttgcggca gttactatgt gttggcgccg 2101 gtgccgcatt taggcaccgc gaacctcgac tggacggttg aggggccaaa actacgcgac 2161 cgtatttttg cgtaccttga gcagcattac atgcctggct tacggagtca gctggtcacg 2221 caccggatgt ttacgccgtt tgattttcgc gaccagctta atgcctatca tggctcagcc 2281 ttttctgtgg agcccgttct tacccagagc gcctggtttc ggccgcataa ccgcgataaa 2341 accattacta atctctacct ggtcggcgca ggcacgcatc ccggcgcagg cattcctggc 2401 gtcatcggct cggcaaaagc gacagcaggt ttgatgctgg aggatctgat ttaataatac 2461 tagagattaa agaggagaaa tactagatgc aaccgcatta tgatctgatt ctcgtggggg 2521 ctggactcgc gaatggcctt atcgccctgc gtcttcagca gcagcaacct gatatgcgta 2581 ttttgcttat cgacgccgca ccccaggcgg gcgggaatca tacgtggtca tttcaccacg 2641 atgatttgac tgagagccaa catcgttgga tagctccgct ggtggttcat cactggcccg 2701 actatcaggt acgctttccc acacgccgtc gtaagctgaa cagcggctac ttttgtatta 2761 cttctcagcg tttcgctgag gttttacagc gacagtttgg cccgcacttg tggatggata 2821 ccgcggtcgc agaggttaat gcggaatctg ttcggttgaa aaagggtcag gttatcggtg 2881 cccgcgcggt gattgacggg cggggttatg cggcaaattc agcactgagc gtgggcttcc 2941 aggcgtttat tggccaggaa tggcgattga gccacccgca tggtttatcg tctcccatta 3001 tcatggatgc cacggtcgat cagcaaaatg gttatcgctt cgtgtacagc ctgccgctct 3061 cgccgaccag attgttaatt gaagacacgc actatattga taatgcgaca ttagatcctg 3121 aatgcgcgcg gcaaaatatt tgcgactatg ccgcgcaaca gggttggcag cttcagacac 3181 tgctgcgaga agaacagggc gccttaccca ttactctgtc gggcaatgcc gacgcattct 3241 ggcagcagcg ccccctggcc tgtagtggat tacgtgccgg tctgttccat cctaccaccg 3301 gctattcact gccgctggcg gttgccgtgg ccgaccgcct gagtgcactt gatgtcttta 3361 cgtcggcctc aattcaccat gccattacgc attttgcccg cgagcgctgg cagcagcagg 3421 gctttttccg catgctgaat cgcatgctgt ttttagccgg acccgccgat tcacgctggc 3481 gggttatgca gcgtttttat ggtttacctg aagatttaat tgcccgtttt tatgcgggaa 3541 aactcacgct gaccgatcgg ctacgtattc tgagcggcaa gccgcctgtt ccggtattag 3601 cagcattgca agccattatg acgactcatc gttaataa //