LOCUS BBa_J29023 8895 bp DNA linear 22-Nov-2024 DEFINITION light sensor and lacI and β-carotene ACCESSION BBa_J29023 VERSION BBa_J29023.1 FEATURES Location/Qualifiers misc_feature 727..751 /label=Help:Barcodes CDS 1..751 /label=BBa_I15008 CDS 758..1507 /label=PcyA misc_feature 1508..1532 /label=Help:Barcodes CDS 758..1532 /label=BBa_I15009 CDS 1539..3776 /label=Cph8 misc_feature 3777..3801 /label=Help:Barcodes CDS 1539..3801 /label=BBa_I15010 misc_feature 3822..3839 /label=C1 OmpR misc_feature 3843..3860 /label=C2 OmpR misc_feature 3863..3880 /label=C3 OmpR promoter 3884..3889 /label=-35 promoter 3907..3912 /label=-10 promoter 3810..3917 /label=BBa_R0082 misc_feature 3930..3933 /label=conserved RBS 3926..3937 /label=BBa_B0034 misc_feature 5033..5071 /label=LVA CDS 3944..5071 /label=lacI-LVA misc_feature 3944..5071 /label=BBa_C0012 misc_feature 5072..5096 /label=Help:Barcodes CDS 3944..5071 /label=BBa_C0012 misc_feature 1..5096 /label=BBa_J29022 misc_feature 5105..5141 /label=P(Lac) IQ promoter 5107..5112 /label=-35 promoter 5130..5135 /label=-10 promoter 5105..5141 /label=BBa_I14032 RBS 5150..5164 /label=RBS-1\Strong RBS 5157..5161 /label=RBS misc_feature 5150..5164 /label=BBa_B0030 RBS 5150..5164 /label=BBa_B0030 RBS 5181..5195 /label=RBS-1\Strong RBS 5188..5192 /label=RBS misc_feature 5181..5195 /label=BBa_B0030 RBS 5181..5195 /label=BBa_B0030 misc_feature 6090..6095 /label=double stop codon CDS 5202..6095 /label=crtB coding sequence CDS 5202..6095 /label=BBa_J29001 RBS 6104..6118 /label=RBS-1\Strong RBS 6111..6115 /label=RBS misc_feature 6104..6118 /label=BBa_B0030 RBS 6104..6118 /label=BBa_B0030 misc_feature 7601..7606 /label=Double stop codon CDS 6125..7606 /label=CrtI coding sequence CDS 6125..7606 /label=BBa_J29002 RBS 7615..7629 /label=RBS-1\Strong RBS 7622..7626 /label=RBS misc_feature 7615..7629 /label=BBa_B0030 RBS 7615..7629 /label=BBa_B0030 CDS 7636..8787 /label=BBa_J29030 misc_feature 5150..8787 /label=BBa_J29021 stem_loop 8808..8830 /label=stem_loop misc_feature 8796..8841 /label=BBa_B0011 terminator 8796..8841 /label=BBa_B0011 stem_loop 8862..8884 /label=stem_loop misc_feature 8850..8895 /label=BBa_B0011 terminator 8850..8895 /label=BBa_B0011 ORIGIN 1 atgagtgtca acttagcttc ccagttgcgg gaagggacga aaaaatccca ctccatggcg 61 gagaacgtcg gctttgtcaa atgcttcctc aagggcgttg tcgagaaaaa ttcctaccgt 121 aagctggttg gcaatctcta ctttgtctac agtgccatgg aagaggaaat ggcaaaattt 181 aaggaccatc ccatcctcag ccacatttac ttccccgaac tcaaccgcaa acaaagccta 241 gagcaagacc tgcaattcta ttacggctcc aactggcggc aagaagtgaa aatttctgcc 301 gctggccaag cctatgtgga ccgagtccgg caagtggccg ctacggcccc tgaattgttg 361 gtggcccatt cctacacccg ttacctgggg gatctttccg gcggtcaaat tctcaagaaa 421 attgcccaaa atgccatgaa tctccacgat ggtggcacag ctttctatga atttgccgac 481 attgatgacg aaaaggcttt taaaaatacc taccgtcaag ctatgaatga tctgcccatt 541 gaccaagcca ccgccgaacg gattgtggat gaagccaatg acgcctttgc catgaacatg 601 aaaatgttca acgaacttga aggcaacctg atcaaggcga tcggcattat ggtgttcaac 661 agcctcaccc gtcgccgcag tcaaggcagc accgaagttg gcctcgccac ctccgaaggc 721 taataacgct gatagtgcta gtgtagatcg ctactagatg gccgtcactg atttaagttt 781 gaccaattct tccctgatgc ctacgttgaa cccgatgatt caacagttgg ccctggcgat 841 cgccgctagt tggcaaagtt tacccctcaa gccctatcaa ttgccggagg atttgggcta 901 cgtagaaggc cgcctggaag gggaaaagtt agtgattgaa aatcggtgct accaaacgcc 961 ccagtttcgc aaaatgcatt tggagttggc caaggtgggc aaagggttgg atattctcca 1021 ctgtgtaatg tttcctgagc ctttatacgg tctacctttg tttggctgtg acattgtggc 1081 cggccccggt ggagtaagtg cggctattgc ggatctatcc cccacccaaa gcgatcgcca 1141 attgcccgca gcgtaccaaa aatcattggc agagctaggc cagccagaat ttgagcaaca 1201 acgggaattg cccccctggg gagaaatatt ttctgaatat tgtttattca tccgtcccag 1261 caatgtcact gaagaagaaa gatttgtaca aagggtagtg gactttttgc aaattcattg 1321 tcaccaatcc atcgttgccg aacccttgtc tgaagctcaa actttggagc accgtcaggg 1381 gcaaattcat tactgccaac aacaacagaa aaatgataaa acccgtcggg tactggaaaa 1441 agcttttggg gaagcttggg cggaacggta tatgagccaa gtcttatttg atgttatcca 1501 ataataactc tgatagtgct agtgtagatc tctactagat ggccaccacc gtacaactca 1561 gcgaccaatc cctccgtcag ctagaaaccc tcgccatcca caccgcccac ctgattcagc 1621 cccacggttt agtggtggtc ctgcaagaac cagacctcac catcagccaa attagcgcca 1681 actgcaccgg cattttaggg cgatcgccag aggatttgtt gggcagaacc ctaggggaag 1741 tgtttgatag ctttcagatt gatcccatcc agagtcgcct aacggccgga caaatcagca 1801 gcctcaaccc cagtaaactt tgggcgcggg tcatggggga cgactttgtc atttttgacg 1861 gggtttttca tcgcaacagt gacggtttat tggtatgtga actcgagcca gcctacactt 1921 ccgataatct gcccttcctc ggtttttatc acatggccaa cgctgccctg aatcggttgc 1981 gccaacaagc taatctacgg gatttctacg atgttattgt cgaagaagtc cgccgtatga 2041 ctggctttga ccgggtgatg ctataccgct ttgatgaaaa taaccacggt gatgtcattg 2101 ccgaagataa acgggatgat atggaaccct atttgggcct gcactatccc gaatcggata 2161 ttccccaacc cgcccgtcgg ctatttatcc acaaccccat tcgagtaatt cccgatgttt 2221 atggtgtggc ggtgcccctg accccagcgg ttaaccccag caccaaccga gcggtggatt 2281 taacagaatc cattctgcgc agtgcgtacc attgccactt gacctatctg aaaaatatgg 2341 gggtaggagc gtctttaacc atttccctaa ttaaggacgg ccatctctgg gggctcattg 2401 cctgccacca tcaaaccccc aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt 2461 ttggtcgggt ggtgtttagc aacatttccg cccaggaaga tacggaaacc ttcgattacc 2521 gggtgcagct ggcggagcat gaagcggttt tattggacaa aatgaccacg gcggcggatt 2581 ttgtcgaagg attaactaat catcccgatc gcctgttggg attaacgggc tcccaggggg 2641 cggccatttg ctttggggaa aaattgattt tagtagggga aaccccggac gagaaagcag 2701 tgcaatattt actgcaatgg ttggagaatc gggaagtgca agacgttttc ttcacctctt 2761 ccctctcaca aatttatcct gatgcagtga attttaaatc cgtggccagt ggcttattgg 2821 ccattcccat tgcccgtcac aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg 2881 ttaattgggg cggtgaccca aatcatgctt acgaagctac ccaggaagac ggtaaaatcg 2941 agctccatcc ccgccaatcc tttgacctct ggaaagaaat tgtccgactc caatctttgc 3001 cctggcaatc ggtggaaatc caaagtgccc tggccctgaa aaaggcgatc gtcaacctca 3061 ttttgcgcca ggcagaagaa ttgcatatgg cggctggtgt taagcaactg gcggatgacc 3121 gcacgctgct gatggcgggg gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc 3181 tggcgactga gatgatgagc gagcaggatg gctatctggc agaatcgatc aataaagata 3241 tcgaagagtg caacgccatc attgagcagt ttatcgacta cctgcgcacc gggcaggaga 3301 tgccgatgga aatggcggat cttaatgcag tactcggtga ggtgattgct gccgaaagtg 3361 gctatgagcg ggaaattgaa accgcgcttt accccggcag cattgaagtg aaaatgcacc 3421 cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg 3481 gctgggtcaa agtcagcagc ggaacggagc cgaatcgcgc ctggttccag gtggaagatg 3541 acggtccggg aattgcgccg gaacaacgta agcacctgtt ccagccgttt gtccgcggcg 3601 acagtgcgcg caccattagc ggcacgggat tagggctggc aattgtgcag cgtatcgtgg 3661 ataaccataa cgggatgctg gagcttggca ccagcgagcg gggcgggctt tccattcgcg 3721 cctggctgcc agtgccggta acgcgggcgc agggcatgac aaaagaaggg taataactct 3781 gatagtgcta gtgtagatct ctactagagt cccttgcatt tacattttga aacatctata 3841 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt 3901 tttggggaga atggacttac tagagaaaga ggagaaatac tagatggtga atgtgaaacc 3961 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 4021 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 4081 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 4141 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 4201 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 4261 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 4321 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 4381 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 4441 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 4501 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 4561 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 4621 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 4681 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 4741 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 4801 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 4861 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 4921 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4981 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa 5041 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact 5101 agagtggtgc aaaacctttc gcggtatggc atgatagcgc ctactagaga ttaaagagga 5161 gaaatactag agtactagag attaaagagg agaaatacta gatggcagtt ggctcgaaaa 5221 gttttgcgac agcctcaaag ttatttgatg caaaaacccg gcgcagcgta ctgatgctct 5281 acgcctggtg ccgccattgt gacgatgtta ttgacgatca gacgctgggc tttcaggccc 5341 ggcagcctgc cttacaaacg cccgaacaac gtctgatgca acttgagatg aaaacgcgcc 5401 aggcctatgc aggatcgcag atgcacgaac cggcgtttgc ggcttttcag gaagtggcta 5461 tggctcatga tatcgccccg gcttacgcgt ttgatcatct ggaaggcttc gccatggatg 5521 tacgcgaagc gcaatacagc caactggatg atacgctgcg ctattgctat cacgttgcag 5581 gcgttgtcgg cttgatgatg gcgcaaatca tgggcgtacg ggataacgcc acgctggacc 5641 gcgcctgtga ccttgggctg gcatttcagt tgaccaatat tgctcgcgat attgtggacg 5701 atgcgcatgc gggccgctgt tatctgccgg caagctggct ggagcatgaa ggtctgaaca 5761 aagagaatta tgcggcacct gaaaaccgtc aggcgctgag ccgtatcgcc cgtcgtttgg 5821 tgcaggaagc agaaccttac tatttgtctg ccacagccgg cctggcaggg ttgcccctgc 5881 gttccgcctg ggcaatcgct acggcgaagc aggtttaccg gaaaataggt gtcaaagttg 5941 aacaggccgg tcagcaagcc tgggatcagc ggcagtcaac gaccacgccc gaaaaattaa 6001 cgctgctgct ggccgcctct ggtcaggccc ttacttcccg gatgcgggct catcctcccc 6061 gccctgcgca tctctggcag cgcccgctct aataatacta gagattaaag aggagaaata 6121 ctagatgaaa ccaactacgg taattggtgc aggcttcggt ggcctggcac tggcaattcg 6181 tctacaagct gcggggatcc ccgtcttact gcttgaacaa cgtgataaac ccggcggtcg 6241 ggcttatgtc tacgaggatc aggggtttac ctttgatgca ggcccgacgg ttatcaccga 6301 tcccagtgcc attgaagaac tgtttgcact ggcaggaaaa cagttaaaag agtatgtcga 6361 actgctgccg gttacgccgt tttaccgcct gtgttgggag tcagggaagg tctttaatta 6421 cgataacgat caaacccggc tcgaagcgca gattcagcag tttaatcccc gcgatgtcga 6481 aggttatcgt cagtttctgg actattcacg cgcggtgttt aaagaaggct atctaaagct 6541 cggtactgtc ccttttttat cgttcagaga catgcttcgc gccgcacctc aactggcgaa 6601 actccaagca tggagaagcg tttacagtaa ggttgccagt tacatcgaag atgaacatct 6661 gcgccaggcg ttttctttcc actcgctgtt ggtgggcggc aatcccttcg ccacctcatc 6721 catttatacg ttgatacacg cgctggagcg tgagtggggc gtctggtttc cgcgtggcgg 6781 caccggcgca ttagttcagg ggatgataaa gctgtttcag gatctgggtg gcgaagtcgt 6841 gttaaacgcc agagtcagcc atatggaaac gacaggaaac aagattgaag ccgtgcattt 6901 agaggacggt cgcaggttcc tgacgcaagc cgtcgcgtca aatgcagatg tggttcatac 6961 ctatcgcgac ctgttaagcc agcaccctgc cgcggttaag cagtccaaca aactccaaac 7021 taagcgcatg agtaactctc tgtctgtgct ctattttggt ttgaatcacc atcatgatca 7081 gctcgcgcat cacacggttt gtttcggccc gcgttaccgc gagctgattg acgaagtttt 7141 taatcatgat ggcctcgcag aggacttctc actttatctg cacgcgccct gtgtcacgga 7201 ttcgtcactg gcgcctgaag gttgcggcag ttactatgtg ttggcgccgg tgccgcattt 7261 aggcaccgcg aacctcgact ggacggttga ggggccaaaa ctacgcgacc gtatttttgc 7321 gtaccttgag cagcattaca tgcctggctt acggagtcag ctggtcacgc accggatgtt 7381 tacgccgttt gattttcgcg accagcttaa tgcctatcat ggctcagcct tttctgtgga 7441 gcccgttctt acccagagcg cctggtttcg gccgcataac cgcgataaaa ccattactaa 7501 tctctacctg gtcggcgcag gcacgcatcc cggcgcaggc attcctggcg tcatcggctc 7561 ggcaaaagcg acagcaggtt tgatgctgga ggatctgatt taataatact agagattaaa 7621 gaggagaaat actagatgca accgcattat gatctgattc tcgtgggggc tggactcgcg 7681 aatggcctta tcgccctgcg tcttcagcag cagcaacctg atatgcgtat tttgcttatc 7741 gacgccgcac cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact 7801 gagagccaac atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta 7861 cgctttccca cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt 7921 ttcgctgagg ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca 7981 gaggttaatg cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg 8041 attgacgggc ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt 8101 ggccaggaat ggcgattgag ccacccgcat ggtttatcgt ctcccattat catggatgcc 8161 acggtcgatc agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga 8221 ttgttaattg aagacacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg 8281 caaaatattt gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa 8341 gaacagggcg ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc 8401 cccctggcct gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg 8461 ccgctggcgg ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca 8521 attcaccatg ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc 8581 atgctgaatc gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag 8641 cgtttttatg gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg 8701 accgatcggc tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa 8761 gccattatga cgactcatcg ttaataatac tagagagaga atataaaaag ccagattatt 8821 aatccggctt ttttattatt ttactagaga gagaatataa aaagccagat tattaatccg 8881 gcttttttat tattt //