##gff-version 3 ##sequence-region BBa_J29023 1 8895 BBa_J29023 . engineered_region 5150 8787 . + 0 ID=BBa_J29021;Name=BBa_J29021 BBa_J29023 . ribosome_entry_site 7615 7629 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J29021 BBa_J29023 . ribosome_entry_site 7622 7626 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_J29023 . ribosome_entry_site 7615 7629 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_J29023 . engineered_region 7615 7629 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_J29023 . ribosome_entry_site 6104 6118 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J29021 BBa_J29023 . ribosome_entry_site 6111 6115 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_J29023 . ribosome_entry_site 6104 6118 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_J29023 . engineered_region 6104 6118 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_J29023 . CDS 6125 7606 . + 0 ID=BBa_J29002;Name=BBa_J29002;Parent=BBa_J29021 BBa_J29023 . stop_codon 7601 7606 . + 0 ID=annotation1892588;Name=Double stop codon;Parent=BBa_J29002 BBa_J29023 . CDS 6125 7606 . + 0 ID=annotation1892591;Name=CrtI coding sequence;Parent=BBa_J29002 BBa_J29023 . CDS 5202 6095 . + 0 ID=BBa_J29001;Name=BBa_J29001;Parent=BBa_J29021 BBa_J29023 . stop_codon 6090 6095 . + 0 ID=annotation1892586;Name=double stop codon;Parent=BBa_J29001 BBa_J29023 . CDS 5202 6095 . + 0 ID=annotation1892590;Name=crtB coding sequence;Parent=BBa_J29001 BBa_J29023 . ribosome_entry_site 5150 5164 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J29021 BBa_J29023 . ribosome_entry_site 5157 5161 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_J29023 . ribosome_entry_site 5150 5164 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_J29023 . engineered_region 5150 5164 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_J29023 . ribosome_entry_site 5181 5195 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J29021 BBa_J29023 . ribosome_entry_site 5188 5192 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_J29023 . ribosome_entry_site 5181 5195 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_J29023 . engineered_region 5181 5195 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_J29023 . CDS 7636 8787 . + 0 ID=BBa_J29030;Name=BBa_J29030;Parent=BBa_J29021 BBa_J29023 . engineered_region 1 5096 . + 0 ID=BBa_J29022;Name=BBa_J29022 BBa_J29023 . ribosome_entry_site 3926 3937 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J29022 BBa_J29023 . conserved_region 3930 3933 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J29023 . CDS 3944 5071 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J29022 BBa_J29023 . engineered_region 3944 5071 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_J29023 . engineered_tag 5072 5096 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_J29023 . tag 5033 5071 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_J29023 . CDS 3944 5071 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_J29023 . CDS 758 1532 . + 0 ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_J29022 BBa_J29023 . CDS 758 1507 . + 0 ID=annotation1891612;Name=PcyA;Parent=BBa_I15009 BBa_J29023 . engineered_tag 1508 1532 . + 0 ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009 BBa_J29023 . CDS 1 751 . + 0 ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_J29022 BBa_J29023 . engineered_tag 727 751 . + 0 ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008 BBa_J29023 . CDS 1539 3801 . + 0 ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_J29022 BBa_J29023 . engineered_tag 3777 3801 . + 0 ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010 BBa_J29023 . CDS 1539 3776 . + 0 ID=annotation1891613;Name=Cph8;Parent=BBa_I15010 BBa_J29023 . promoter 3810 3917 . + 0 ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_J29022 BBa_J29023 . promoter 3884 3889 . + 0 ID=annotation301166;Name=-35;Parent=BBa_R0082 BBa_J29023 . promoter 3907 3912 . + 0 ID=annotation301167;Name=-10;Parent=BBa_R0082 BBa_J29023 . non_covalent_binding_site 3863 3880 . + 0 ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082 BBa_J29023 . non_covalent_binding_site 3822 3839 . + 0 ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082 BBa_J29023 . non_covalent_binding_site 3843 3860 . + 0 ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082 BBa_J29023 . terminator 8850 8895 . + 0 ID=BBa_B0011;Name=BBa_B0011 BBa_J29023 . stem_loop 8862 8884 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_J29023 . engineered_region 8850 8895 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_J29023 . terminator 8796 8841 . + 0 ID=BBa_B0011;Name=BBa_B0011 BBa_J29023 . stem_loop 8808 8830 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_J29023 . engineered_region 8796 8841 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_J29023 . promoter 5105 5141 . + 0 ID=BBa_I14032;Name=BBa_I14032 BBa_J29023 . operator 5105 5141 . + 0 ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032 BBa_J29023 . promoter 5107 5112 . + 0 ID=annotation1028343;Name=-35;Parent=BBa_I14032 BBa_J29023 . promoter 5130 5135 . + 0 ID=annotation1028344;Name=-10;Parent=BBa_I14032 >BBa_J29023 atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtca aatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccat ggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagc ctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatg tggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatct ttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaattt gccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccg ccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgat caaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacc tccgaaggctaataacgctgatagtgctagtgtagatcgctactagatggccgtcactgatttaagtttgaccaattct tccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctca agccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtg ctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgta atgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggcta ttgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagcc agaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaat gtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaac ccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaac ccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaa taataactctgatagtgctagtgtagatctctactagatggccaccaccgtacaactcagcgaccaatccctccgtcag ctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctca ccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctagggga agtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaa ctttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtat gtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcg gttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgg gtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatt tgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcc cgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatcc attctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaa ttaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagc ctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcag ctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccg atcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaacccc ggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctc tcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaact ttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctaccca ggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccc tggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaat tgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcac gccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagat atcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcgg atcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccgg cagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggc aatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattg cgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagg gctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttcc attcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagt gtagatctctactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagt atcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtga atgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgct ttagtagcttaataactctgatagtgctagtgtagatctctactagagtggtgcaaaacctttcgcggtatggcatgat agcgcctactagagattaaagaggagaaatactagagtactagagattaaagaggagaaatactagatggcagttggct cgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccg ccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgt ctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcagg aagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagc gcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatc atgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcg atattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaa ttatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttg tctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaa taggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgct gctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgc ccgctctaataatactagagattaaagaggagaaatactagatgaaaccaactacggtaattggtgcaggcttcggtgg cctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgg gcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaac tgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttggga gtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtc gaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttt tatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactccaagcatggagaagcgtttacagtaaggttgc cagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacc tcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattag ttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgac aggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtg gttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactccaaactaagcgcatga gtaactctctgtctgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggccc gcgttaccgcgagctgattgacgaagtttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccc tgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccg cgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctgg cttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctca gccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctct acctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgat gctggaggatctgatttaataatactagagattaaagaggagaaatactagatgcaaccgcattatgatctgattctcg tgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcga cgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggata gctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctact tttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgc agaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttat gcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttat cgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgac cagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgac tatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggca atgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggcta ttcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgcc attacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggac ccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaa actcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatg acgactcatcgttaataatactagagagagaatataaaaagccagattattaatccggcttttttattattttactaga gagagaatataaaaagccagattattaatccggcttttttattattt