BBa_J29023
type: ComponentDefinition
persistent identity: BBa J29023
display id: BBa_J29023
version: 1
was generated by: iGEM to SBOL conversion
description: light sensor and lacI and β-carotene
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: It's from plants.
owned by: james
owned by: myers
mutable description: Carene cyclase is coded.
discontinued: false
dominant: false
experience: experience/None
group u list: _59_
m user id: 0
owner id: 892
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It makes Aroma.
star: false
creator: Mayu Ikezumi
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_I14032
component: BBa_J29022
component: BBa_J29021
sequence annotation: BBa_J29022
sequence annotation: BBa_I14032
sequence annotation: BBa_J29021
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_J29023_sequence/1

BBa_B0011
type: Component
persistent identity: component2314700
display id: component2314700
version: 1
top level: BBa_J29023
definition: BBa_B0011
access: public

BBa_I14032
type: Component
persistent identity: component2314677
display id: component2314677
version: 1
top level: BBa_J29023
definition: P(Lac) IQ
access: public

BBa_J29022
type: Component
persistent identity: component2314673
display id: component2314673
version: 1
top level: BBa_J29023
definition: BBa_J29022
access: public

BBa_B0011
type: Component
persistent identity: component2314698
display id: component2314698
version: 1
top level: BBa_J29023
definition: BBa_B0011
access: public

BBa_J29021
type: Component
persistent identity: component2314697
display id: component2314697
version: 1
top level: BBa_J29023
definition: BBa_J29021
access: public

BBa_J29022
type: SequenceAnnotation
persistent identity: annotation2314673
display id: annotation2314673
version: 1
top level: BBa_J29023
location: https://synbiohub.org/public/igem/BBa_J29023/annotation2314673/range2314673/1
component: BBa_J29022

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type: Range
persistent identity: range2314673
display id: range2314673
version: 1
top level: BBa_J29023
direction: direction/reverse
start: 1
end: 5096
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2314677
display id: annotation2314677
version: 1
top level: BBa_J29023
location: https://synbiohub.org/public/igem/BBa_J29023/annotation2314677/range2314677/1
component: BBa_I14032

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type: Range
persistent identity: range2314677
display id: range2314677
version: 1
top level: BBa_J29023
direction: direction/reverse
start: 5105
end: 5141
orientation: inline

BBa_J29021
type: SequenceAnnotation
persistent identity: annotation2314697
display id: annotation2314697
version: 1
top level: BBa_J29023
location: https://synbiohub.org/public/igem/BBa_J29023/annotation2314697/range2314697/1
component: BBa_J29021

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type: Range
persistent identity: range2314697
display id: range2314697
version: 1
top level: BBa_J29023
direction: direction/reverse
start: 5150
end: 8787
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2314698
display id: annotation2314698
version: 1
top level: BBa_J29023
location: https://synbiohub.org/public/igem/BBa_J29023/annotation2314698/range2314698/1
component: BBa_B0011

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type: Range
persistent identity: range2314698
display id: range2314698
version: 1
top level: BBa_J29023
direction: direction/forward
start: 8796
end: 8841
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2314700
display id: annotation2314700
version: 1
top level: BBa_J29023
location: https://synbiohub.org/public/igem/BBa_J29023/annotation2314700/range2314700/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_J29023/annotation2314700/range2314700/1
type: Range
persistent identity: range2314700
display id: range2314700
version: 1
top level: BBa_J29023
direction: direction/forward
start: 8850
end: 8895
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

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type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

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type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

BBa_J29021
type: ComponentDefinition
persistent identity: BBa J29021
display id: BBa_J29021
version: 1
was generated by: iGEM to SBOL conversion
description: This is 4 enzymes that is used to produce a β-carotene.
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: The 3 enzymes are CrtE(BBa_J29000), CreB(BBa_J29001), and CrtI(BBa_J29002). The 3 RBS BBa_B0030.
owned by: james
owned by: myers
mutable description: This is 3 enzymes that is used to produce a lycopene(tomato plgment). It also has 3 RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _59_
m user id: 0
owner id: 892
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It uses a storong RBS.
star: false
creator: Mayu Ikezumi
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0030
component: BBa_J29001
component: BBa_J29002
component: BBa_J29030
sequence annotation: BBa_B0030
sequence annotation: BBa_J29001
sequence annotation: BBa_J29002
sequence annotation: BBa_J29030
sequence: https://synbiohub.org/public/igem/BBa_J29021_sequence/1

BBa_B0030
type: Component
persistent identity: component2226250
display id: component2226250
version: 1
top level: BBa_J29021
definition: BBa_B0030
access: public

BBa_J29001
type: Component
persistent identity: component2226257
display id: component2226257
version: 1
top level: BBa_J29021
definition: BBa_J29001
access: public

BBa_B0030
type: Component
persistent identity: component2226253
display id: component2226253
version: 1
top level: BBa_J29021
definition: BBa_B0030
access: public

BBa_J29002
type: Component
persistent identity: component2226263
display id: component2226263
version: 1
top level: BBa_J29021
definition: BBa_J29002
access: public

BBa_B0030
type: Component
persistent identity: component2226259
display id: component2226259
version: 1
top level: BBa_J29021
definition: BBa_B0030
access: public

BBa_J29030
type: Component
persistent identity: component2226267
display id: component2226267
version: 1
top level: BBa_J29021
definition: BBa_J29030
access: public

BBa_B0030
type: Component
persistent identity: component2226265
display id: component2226265
version: 1
top level: BBa_J29021
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2226250
display id: annotation2226250
version: 1
top level: BBa_J29021
location: https://synbiohub.org/public/igem/BBa_J29021/annotation2226250/range2226250/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J29021/annotation2226250/range2226250/1
type: Range
persistent identity: range2226250
display id: range2226250
version: 1
top level: BBa_J29021
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2226253
display id: annotation2226253
version: 1
top level: BBa_J29021
location: https://synbiohub.org/public/igem/BBa_J29021/annotation2226253/range2226253/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J29021/annotation2226253/range2226253/1
type: Range
persistent identity: range2226253
display id: range2226253
version: 1
top level: BBa_J29021
direction: direction/forward
start: 32
end: 46
orientation: inline

BBa_J29001
type: SequenceAnnotation
persistent identity: annotation2226257
display id: annotation2226257
version: 1
top level: BBa_J29021
location: https://synbiohub.org/public/igem/BBa_J29021/annotation2226257/range2226257/1
component: BBa_J29001

https://synbiohub.org/public/igem/BBa_J29021/annotation2226257/range2226257/1
type: Range
persistent identity: range2226257
display id: range2226257
version: 1
top level: BBa_J29021
direction: direction/reverse
start: 53
end: 946
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2226259
display id: annotation2226259
version: 1
top level: BBa_J29021
location: https://synbiohub.org/public/igem/BBa_J29021/annotation2226259/range2226259/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J29021/annotation2226259/range2226259/1
type: Range
persistent identity: range2226259
display id: range2226259
version: 1
top level: BBa_J29021
direction: direction/forward
start: 955
end: 969
orientation: inline

BBa_J29002
type: SequenceAnnotation
persistent identity: annotation2226263
display id: annotation2226263
version: 1
top level: BBa_J29021
location: https://synbiohub.org/public/igem/BBa_J29021/annotation2226263/range2226263/1
component: BBa_J29002

https://synbiohub.org/public/igem/BBa_J29021/annotation2226263/range2226263/1
type: Range
persistent identity: range2226263
display id: range2226263
version: 1
top level: BBa_J29021
direction: direction/reverse
start: 976
end: 2457
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2226265
display id: annotation2226265
version: 1
top level: BBa_J29021
location: https://synbiohub.org/public/igem/BBa_J29021/annotation2226265/range2226265/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J29021/annotation2226265/range2226265/1
type: Range
persistent identity: range2226265
display id: range2226265
version: 1
top level: BBa_J29021
direction: direction/forward
start: 2466
end: 2480
orientation: inline

BBa_J29030
type: SequenceAnnotation
persistent identity: annotation2226267
display id: annotation2226267
version: 1
top level: BBa_J29021
location: https://synbiohub.org/public/igem/BBa_J29021/annotation2226267/range2226267/1
component: BBa_J29030

https://synbiohub.org/public/igem/BBa_J29021/annotation2226267/range2226267/1
type: Range
persistent identity: range2226267
display id: range2226267
version: 1
top level: BBa_J29021
direction: direction/reverse
start: 2487
end: 3638
orientation: inline

BBa_J29022
type: ComponentDefinition
persistent identity: BBa J29022
display id: BBa_J29022
version: 1
was generated by: iGEM to SBOL conversion
description: light receptor and LacI
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: light receptor is made of I15008,I15009,I15010,and R0082. LacI is C0012.
owned by: james
owned by: myers
mutable description: light receptor and LacI
discontinued: false
dominant: false
group u list: _59_
m user id: 0
owner id: 891
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: RBS is very strong.
star: false
creator: Daisuke Abe
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I15009
component: BBa_I15008
component: BBa_R0082
component: BBa_I15010
component: BBa_C0012
component: BBa_B0034
sequence annotation: BBa_I15008
sequence annotation: BBa_I15009
sequence annotation: BBa_I15010
sequence annotation: BBa_R0082
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_J29022_sequence/1

BBa_I15009
type: Component
persistent identity: component2260038
display id: component2260038
version: 1
top level: BBa_J29022
definition: BBa_I15009
access: public

BBa_I15008
type: Component
persistent identity: component2260035
display id: component2260035
version: 1
top level: BBa_J29022
definition: BBa_I15008
access: public

BBa_R0082
type: Component
persistent identity: component2260047
display id: component2260047
version: 1
top level: BBa_J29022
definition: OmpR
access: public

BBa_I15010
type: Component
persistent identity: component2260041
display id: component2260041
version: 1
top level: BBa_J29022
definition: BBa_I15010
access: public

BBa_C0012
type: Component
persistent identity: component2260051
display id: component2260051
version: 1
top level: BBa_J29022
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component2260049
display id: component2260049
version: 1
top level: BBa_J29022
definition: BBa_B0034
access: public

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2260035
display id: annotation2260035
version: 1
top level: BBa_J29022
location: https://synbiohub.org/public/igem/BBa_J29022/annotation2260035/range2260035/1
component: BBa_I15008

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type: Range
persistent identity: range2260035
display id: range2260035
version: 1
top level: BBa_J29022
direction: direction/reverse
start: 1
end: 751
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2260038
display id: annotation2260038
version: 1
top level: BBa_J29022
location: https://synbiohub.org/public/igem/BBa_J29022/annotation2260038/range2260038/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_J29022/annotation2260038/range2260038/1
type: Range
persistent identity: range2260038
display id: range2260038
version: 1
top level: BBa_J29022
direction: direction/reverse
start: 758
end: 1532
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation2260041
display id: annotation2260041
version: 1
top level: BBa_J29022
location: https://synbiohub.org/public/igem/BBa_J29022/annotation2260041/range2260041/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_J29022/annotation2260041/range2260041/1
type: Range
persistent identity: range2260041
display id: range2260041
version: 1
top level: BBa_J29022
direction: direction/reverse
start: 1539
end: 3801
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2260047
display id: annotation2260047
version: 1
top level: BBa_J29022
location: https://synbiohub.org/public/igem/BBa_J29022/annotation2260047/range2260047/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_J29022/annotation2260047/range2260047/1
type: Range
persistent identity: range2260047
display id: range2260047
version: 1
top level: BBa_J29022
direction: direction/reverse
start: 3810
end: 3917
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2260049
display id: annotation2260049
version: 1
top level: BBa_J29022
location: https://synbiohub.org/public/igem/BBa_J29022/annotation2260049/range2260049/1
component: BBa_B0034

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type: Range
persistent identity: range2260049
display id: range2260049
version: 1
top level: BBa_J29022
direction: direction/reverse
start: 3926
end: 3937
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2260051
display id: annotation2260051
version: 1
top level: BBa_J29022
location: https://synbiohub.org/public/igem/BBa_J29022/annotation2260051/range2260051/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J29022/annotation2260051/range2260051/1
type: Range
persistent identity: range2260051
display id: range2260051
version: 1
top level: BBa_J29022
direction: direction/forward
start: 3944
end: 5071
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: iGEM to SBOL conversion
description: heme oxygenase (ho1) from Synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
top level: BBa_I15008
direction: direction/forward
start: 727
end: 751
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: iGEM to SBOL conversion
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Second of two required phycocyanobilin biosynthetic genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
top level: BBa_I15009
direction: direction/forward
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
top level: BBa_I15009
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: iGEM to SBOL conversion
description: cph8 (Cph1/EnvZ fusion)
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
top level: BBa_I15010
direction: direction/forward
start: 1
end: 2238
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
top level: BBa_I15010
direction: direction/forward
start: 2239
end: 2263
orientation: inline

BBa_J29001
type: ComponentDefinition
persistent identity: BBa J29001
display id: BBa_J29001
version: 1
was generated by: iGEM to SBOL conversion
description: CrtB
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: This part comes from Erwinia uredovora. This squences comes from (a detabase).
owned by: james
owned by: myers
mutable description: CrtB is the second of 3 an enzyme that is use to produce a lycopene(tomato plgment). Use it with J
discontinued: true
dominant: false
experience: experience/None
group u list: _59_
m user id: 0
owner id: 892
owning group id: 59
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: I replaced the TAG stop codon to TAATAA.
star: false
creator: Mayu Ikezumi
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtB coding sequence
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_J29001_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1892590
display id: annotation1892590
version: 1
top level: BBa_J29001
location: https://synbiohub.org/public/igem/BBa_J29001/annotation1892590/range1892590/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J29001/annotation1892590/range1892590/1
type: Range
persistent identity: range1892590
display id: range1892590
version: 1
top level: BBa_J29001
direction: direction/forward
start: 1
end: 894
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation1892586
display id: annotation1892586
version: 1
top level: BBa_J29001
location: https://synbiohub.org/public/igem/BBa_J29001/annotation1892586/range1892586/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J29001/annotation1892586/range1892586/1
type: Range
persistent identity: range1892586
display id: range1892586
version: 1
top level: BBa_J29001
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_J29002
type: ComponentDefinition
persistent identity: BBa J29002
display id: BBa_J29002
version: 1
was generated by: iGEM to SBOL conversion
description: CrtI
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: This part come from Erwinia uredovora.
owned by: james
owned by: myers
mutable description: CrtI is the third of 3 enzyme that is used to produce lycopene (tomato pigment).
discontinued: false
dominant: false
group u list: _59_
m user id: 0
owner id: 880
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I changed a stop codon TGA to TAA TAA.
star: false
creator: Keiichi Suzuki
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CrtI coding sequence
sequence annotation: Double stop codon
sequence: https://synbiohub.org/public/igem/BBa_J29002_sequence/1

CrtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1892591
display id: annotation1892591
version: 1
top level: BBa_J29002
location: https://synbiohub.org/public/igem/BBa_J29002/annotation1892591/range1892591/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J29002/annotation1892591/range1892591/1
type: Range
persistent identity: range1892591
display id: range1892591
version: 1
top level: BBa_J29002
direction: direction/forward
start: 1
end: 1482
orientation: inline

Double stop codon
type: SequenceAnnotation
persistent identity: annotation1892588
display id: annotation1892588
version: 1
top level: BBa_J29002
location: https://synbiohub.org/public/igem/BBa_J29002/annotation1892588/range1892588/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J29002/annotation1892588/range1892588/1
type: Range
persistent identity: range1892588
display id: range1892588
version: 1
top level: BBa_J29002
direction: direction/forward
start: 1477
end: 1482
orientation: inline

BBa_J29030
type: ComponentDefinition
persistent identity: BBa J29030
display id: BBa_J29030
version: 1
was generated by: iGEM to SBOL conversion
description: CrtY
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: This part comes from Erwinia uredovora.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: CrtY is the last of 4 enzymes that is used to produce a beta-carotene.
discontinued: false
dominant: true
group u list: _59_
m user id: 0
owner id: 892
owning group id: 59
sample status: In stock
status: status/Available
bookmark: false
mutable notes: It changes from lycopene to beta-carotene.
star: false
creator: Mayu Ikezumi
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J29030_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

https://synbiohub.org/public/igem/BBa_J29023_sequence/1
type: Sequence
persistent identity: BBa J29023 sequence
display id: BBa_J29023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J29021_sequence/1
type: Sequence
persistent identity: BBa J29021 sequence
display id: BBa_J29021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagagtactagagattaaagaggagaaatactagatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagattaaagaggagaaatactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactccaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactccaaactaagcgcatgagtaactctctgtctgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaagtttttaatcatga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J29022_sequence/1
type: Sequence
persistent identity: BBa J29022 sequence
display id: BBa_J29022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15008_sequence/1
type: Sequence
persistent identity: BBa I15008 sequence
display id: BBa_I15008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15009_sequence/1
type: Sequence
persistent identity: BBa I15009 sequence
display id: BBa_I15009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15010_sequence/1
type: Sequence
persistent identity: BBa I15010 sequence
display id: BBa_I15010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J29001_sequence/1
type: Sequence
persistent identity: BBa J29001 sequence
display id: BBa_J29001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J29002_sequence/1
type: Sequence
persistent identity: BBa J29002 sequence
display id: BBa_J29002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactccaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactccaaactaagcgcatgagtaactctctgtctgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaagtttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J29030_sequence/1
type: Sequence
persistent identity: BBa J29030 sequence
display id: BBa_J29030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z