LOCUS BBa_J29034 3273 bp DNA linear 23-Nov-2024 DEFINITION PBPB expression system ACCESSION BBa_J29034 VERSION BBa_J29034.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 RBS 117..131 /label=RBS-1\Strong RBS 124..128 /label=RBS misc_feature 117..131 /label=BBa_B0030 RBS 117..131 /label=BBa_B0030 CDS 138..2042 /label=BBa_J29029 misc_feature 2051..3136 /label=BBa_J29032 stem_loop 3156..3199 /label=stem_loop misc_feature 3145..3224 /label=BBa_B0010 terminator 3145..3224 /label=BBa_B0010 stem_loop 3240..3259 /label=T7 TE misc_feature 3266..3266 /label=stop polyA_site 3260..3273 /label=polya misc_feature 3233..3273 /label=BBa_B0012 terminator 3233..3273 /label=BBa_B0012 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagatta 121 aagaggagaa atactagatg aaactacaga actcttttcg cgactatacg gcagagtccg 181 cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat tttgctgctg accggcgtgc 241 ttatcgccaa cctgtataat ctgcaaattg ttcgctttac cgactaccag acccgctcta 301 atgaaaaccg cattaagctg gtgcctatcg cgcccagccg cggcattatc tacgatcgta 361 acggtatccc tctggccctc aaccgcacta tctaccagat agaaatgatg ccggagaaag 421 tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt ggtagatctg accgatgacg 481 atattgctgc attccgaaaa gagcgcgcac gttcacaccg tttcacctct attccggtga 541 aaactaacct gaccgaagta caagtagctc gctttgccgt caatcagtac cgttttccgg 601 gtgtcgaagt taaaggctat aaacgtcgtt actatcctta cggttcggcg ttgacccacg 661 tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat aatgacggca 721 aactggccaa ctatgcggca acgcatgata tcggtaagct gggcattgag cgttactatg 781 aagatgtgct gcacggtcag accggttatg aagaggttga agttaacaac cgtgggcgtg 841 ttattcgcca gttaaaagaa gtaccaccgc aagccggaca cgatatttac ctgacgctgg 901 atctcaaact ccagcaatat attgaaacgc tgctggcggg tagccgcgca gctgtggtag 961 tcaccgatcc gcgtacaggt ggggtgctgg cgctggtttc cacgcctagt tatgacccaa 1021 acttgtttgt tgacggtatc tccagcaaag attattccgc cttgttgaac gatccgaata 1081 caccgctggt gaaccgcgcc acacaggggg tttatcctcc cgcgtctaca gttaaaccct 1141 atgtggcggt ttcggcattg agcgccgggg tgatcacgcg caatacgacg ctgtttgacc 1201 caggctggtg gcaactgcca ggttcggaaa aacgttatcg tgactggaaa aaatggggcc 1261 acgggcgtct gaatgtcaca agatcgctgg aagaatctgc ggataccttc ttctatcagg 1321 tggcctacga tatggggatc gatcgcctct ccgaatggat gggtaaattc ggttatggtc 1381 attacaccgg tatcgacctg gcggaagaac gttccggcaa catgcctacc cgcgaatgga 1441 aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac cattccggtt ggtatcggtc 1501 agggttactg gacagcgacc ccaatccaga tgagtaaggc actgatgatc ctgattaatg 1561 acggtatcgt gaaggttcct catttgctga tgagcaccgc cgaagacggc aaacaggtgc 1621 catgggtaca gccgcatgaa ccgcccgtcg gcgatattca ttccggttac tgggagctgg 1681 cgaaagacgg tatgtacggt gttgctaacc gccctaacgg tacggcgcat aaatactttg 1741 ctagcgcacc gtacaaaatt gcggcgaaat ccggtaccgc tcaggtcttc ggtctgaaag 1801 cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt acgtgaccac aaactgatga 1861 ccgcctttgc gccatacaac aatccgcaag tggctgtcgc catgattctg gagaacggtg 1921 gtgcgggtcc ggcggttggt acactgatgc gccagatcct cgaccacatt atgctgggtg 1981 ataacaacac cgatctgcct gcggaaaatc cagcggttgc cgcagcggag gaccattaat 2041 aatactagag gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 2101 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 2161 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 2221 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 2281 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 2341 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 2401 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 2461 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 2521 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 2581 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 2641 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 2701 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 2761 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 2821 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 2881 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 2941 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 3001 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 3061 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 3121 aagcgggcag taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 3241 gctcaccttc gggtgggcct ttctgcgttt ata //