##gff-version 3
##sequence-region BBa_J29034 1 3273
BBa_J29034	.	engineered_region	2051	3136	.	+	0	ID=BBa_J29032;Name=BBa_J29032
BBa_J29034	.	terminator	3145	3224	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J29034	.	stem_loop	3156	3199	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29034	.	engineered_region	3145	3224	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29034	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_J29034	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J29034	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J29034	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J29034	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J29034	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J29034	.	CDS	138	2042	.	+	0	ID=BBa_J29029;Name=BBa_J29029
BBa_J29034	.	ribosome_entry_site	117	131	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J29034	.	engineered_region	117	131	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J29034	.	ribosome_entry_site	124	128	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J29034	.	ribosome_entry_site	117	131	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J29034	.	terminator	3233	3273	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J29034	.	engineered_region	3233	3273	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29034	.	stop_codon	3266	3266	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29034	.	stem_loop	3240	3259	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29034	.	polyA_site	3260	3273	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J29034
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagattaaagaggagaaatactagatgaaactacagaactctttt
cgcgactatacggcagagtccgcgctgtttgtgcgccgggcgctggtcgcctttttggggattttgctgctgaccggcg
tgcttatcgccaacctgtataatctgcaaattgttcgctttaccgactaccagacccgctctaatgaaaaccgcattaa
gctggtgcctatcgcgcccagccgcggcattatctacgatcgtaacggtatccctctggccctcaaccgcactatctac
cagatagaaatgatgccggagaaagtcgataacgtgcagcaaacgctggacgctttgcgcagcgtggtagatctgaccg
atgacgatattgctgcattccgaaaagagcgcgcacgttcacaccgtttcacctctattccggtgaaaactaacctgac
cgaagtacaagtagctcgctttgccgtcaatcagtaccgttttccgggtgtcgaagttaaaggctataaacgtcgttac
tatccttacggttcggcgttgacccacgtcatcggctatgtgtcgaaaatcaacgataaagacgtcgaacgcctgaata
atgacggcaaactggccaactatgcggcaacgcatgatatcggtaagctgggcattgagcgttactatgaagatgtgct
gcacggtcagaccggttatgaagaggttgaagttaacaaccgtgggcgtgttattcgccagttaaaagaagtaccaccg
caagccggacacgatatttacctgacgctggatctcaaactccagcaatatattgaaacgctgctggcgggtagccgcg
cagctgtggtagtcaccgatccgcgtacaggtggggtgctggcgctggtttccacgcctagttatgacccaaacttgtt
tgttgacggtatctccagcaaagattattccgccttgttgaacgatccgaatacaccgctggtgaaccgcgccacacag
ggggtttatcctcccgcgtctacagttaaaccctatgtggcggtttcggcattgagcgccggggtgatcacgcgcaata
cgacgctgtttgacccaggctggtggcaactgccaggttcggaaaaacgttatcgtgactggaaaaaatggggccacgg
gcgtctgaatgtcacaagatcgctggaagaatctgcggataccttcttctatcaggtggcctacgatatggggatcgat
cgcctctccgaatggatgggtaaattcggttatggtcattacaccggtatcgacctggcggaagaacgttccggcaaca
tgcctacccgcgaatggaaacagaaacgctttaaaaaaccgtggtatcagggtgacaccattccggttggtatcggtca
gggttactggacagcgaccccaatccagatgagtaaggcactgatgatcctgattaatgacggtatcgtgaaggttcct
catttgctgatgagcaccgccgaagacggcaaacaggtgccatgggtacagccgcatgaaccgcccgtcggcgatattc
attccggttactgggagctggcgaaagacggtatgtacggtgttgctaaccgccctaacggtacggcgcataaatactt
tgctagcgcaccgtacaaaattgcggcgaaatccggtaccgctcaggtcttcggtctgaaagcgaacgaaacctataat
gcgcacaaaattgccgagcgtttacgtgaccacaaactgatgaccgcctttgcgccatacaacaatccgcaagtggctg
tcgccatgattctggagaacggtggtgcgggtccggcggttggtacactgatgcgccagatcctcgaccacattatgct
gggtgataacaacaccgatctgcctgcggaaaatccagcggttgccgcagcggaggaccattaataatactagaggtga
aaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccag
ccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaa
caactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcg
cggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctg
taaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgcc
attgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtatta
ttttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagc
gggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccg
atagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttc
ccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgg
tgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacag
gattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagc
tgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata