BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301155 1 C2 OmpR range301155 1 34 51 annotation301156 1 C3 OmpR range301156 1 54 71 annotation301167 1 -10 range301167 1 98 103 annotation301166 1 -35 range301166 1 75 80 annotation301154 1 C1 OmpR range301154 1 13 30 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J29032 1 BBa_J29032 LacI 2006-08-03T11:00:00Z 2015-08-31T04:08:45Z energy metabolism of E. coli LacI is a lactose operon repressor. false true _59_ 0 879 59 Not in stock false I changed a stopcodon TGA to TAATAA. false Kayo Yasuno BBa_J29034 1 BBa_J29034 PBPB expression system 2006-08-03T11:00:00Z 2015-08-31T04:08:45Z E. coli When OmpR promoter turns on, PBPB and Lac repressor are expressed. false false _59_ 0 879 59 Not in stock false OmpR promoter->RBS->pbpB->laci->terminater false Kayo Yasuno component1893540 1 BBa_J29029 component1893538 1 BBa_B0030 component1893542 1 BBa_B0010 component1893536 1 BBa_R0082 component1893544 1 BBa_B0012 component1893541 1 BBa_J29032 annotation1893538 1 BBa_B0030 range1893538 1 117 131 annotation1893544 1 BBa_B0012 range1893544 1 3233 3273 annotation1893536 1 BBa_R0082 range1893536 1 1 108 annotation1893541 1 BBa_J29032 range1893541 1 2051 3136 annotation1893540 1 BBa_J29029 range1893540 1 138 2042 annotation1893542 1 BBa_B0010 range1893542 1 3145 3224 BBa_J29029 1 BBa_J29029 pbpA 2006-08-03T11:00:00Z 2015-08-31T04:08:45Z cell envelope of E. coli PBP2 is a penicillin-binding protein. false false _59_ 0 879 59 Not in stock false I added stopcodons TAATAA. false Kayo Yasuno BBa_J29032_sequence 1 gtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J29029_sequence 1 atgaaactacagaactcttttcgcgactatacggcagagtccgcgctgtttgtgcgccgggcgctggtcgcctttttggggattttgctgctgaccggcgtgcttatcgccaacctgtataatctgcaaattgttcgctttaccgactaccagacccgctctaatgaaaaccgcattaagctggtgcctatcgcgcccagccgcggcattatctacgatcgtaacggtatccctctggccctcaaccgcactatctaccagatagaaatgatgccggagaaagtcgataacgtgcagcaaacgctggacgctttgcgcagcgtggtagatctgaccgatgacgatattgctgcattccgaaaagagcgcgcacgttcacaccgtttcacctctattccggtgaaaactaacctgaccgaagtacaagtagctcgctttgccgtcaatcagtaccgttttccgggtgtcgaagttaaaggctataaacgtcgttactatccttacggttcggcgttgacccacgtcatcggctatgtgtcgaaaatcaacgataaagacgtcgaacgcctgaataatgacggcaaactggccaactatgcggcaacgcatgatatcggtaagctgggcattgagcgttactatgaagatgtgctgcacggtcagaccggttatgaagaggttgaagttaacaaccgtgggcgtgttattcgccagttaaaagaagtaccaccgcaagccggacacgatatttacctgacgctggatctcaaactccagcaatatattgaaacgctgctggcgggtagccgcgcagctgtggtagtcaccgatccgcgtacaggtggggtgctggcgctggtttccacgcctagttatgacccaaacttgtttgttgacggtatctccagcaaagattattccgccttgttgaacgatccgaatacaccgctggtgaaccgcgccacacagggggtttatcctcccgcgtctacagttaaaccctatgtggcggtttcggcattgagcgccggggtgatcacgcgcaatacgacgctgtttgacccaggctggtggcaactgccaggttcggaaaaacgttatcgtgactggaaaaaatggggccacgggcgtctgaatgtcacaagatcgctggaagaatctgcggataccttcttctatcaggtggcctacgatatggggatcgatcgcctctccgaatggatgggtaaattcggttatggtcattacaccggtatcgacctggcggaagaacgttccggcaacatgcctacccgcgaatggaaacagaaacgctttaaaaaaccgtggtatcagggtgacaccattccggttggtatcggtcagggttactggacagcgaccccaatccagatgagtaaggcactgatgatcctgattaatgacggtatcgtgaaggttcctcatttgctgatgagcaccgccgaagacggcaaacaggtgccatgggtacagccgcatgaaccgcccgtcggcgatattcattccggttactgggagctggcgaaagacggtatgtacggtgttgctaaccgccctaacggtacggcgcataaatactttgctagcgcaccgtacaaaattgcggcgaaatccggtaccgctcaggtcttcggtctgaaagcgaacgaaacctataatgcgcacaaaattgccgagcgtttacgtgaccacaaactgatgaccgcctttgcgccatacaacaatccgcaagtggctgtcgccatgattctggagaacggtggtgcgggtccggcggttggtacactgatgcgccagatcctcgaccacattatgctgggtgataacaacaccgatctgcctgcggaaaatccagcggttgccgcagcggaggaccattaataa BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_J29034_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagattaaagaggagaaatactagatgaaactacagaactcttttcgcgactatacggcagagtccgcgctgtttgtgcgccgggcgctggtcgcctttttggggattttgctgctgaccggcgtgcttatcgccaacctgtataatctgcaaattgttcgctttaccgactaccagacccgctctaatgaaaaccgcattaagctggtgcctatcgcgcccagccgcggcattatctacgatcgtaacggtatccctctggccctcaaccgcactatctaccagatagaaatgatgccggagaaagtcgataacgtgcagcaaacgctggacgctttgcgcagcgtggtagatctgaccgatgacgatattgctgcattccgaaaagagcgcgcacgttcacaccgtttcacctctattccggtgaaaactaacctgaccgaagtacaagtagctcgctttgccgtcaatcagtaccgttttccgggtgtcgaagttaaaggctataaacgtcgttactatccttacggttcggcgttgacccacgtcatcggctatgtgtcgaaaatcaacgataaagacgtcgaacgcctgaataatgacggcaaactggccaactatgcggcaacgcatgatatcggtaagctgggcattgagcgttactatgaagatgtgctgcacggtcagaccggttatgaagaggttgaagttaacaaccgtgggcgtgttattcgccagttaaaagaagtaccaccgcaagccggacacgatatttacctgacgctggatctcaaactccagcaatatattgaaacgctgctggcgggtagccgcgcagctgtggtagtcaccgatccgcgtacaggtggggtgctggcgctggtttccacgcctagttatgacccaaacttgtttgttgacggtatctccagcaaagattattccgccttgttgaacgatccgaatacaccgctggtgaaccgcgccacacagggggtttatcctcccgcgtctacagttaaaccctatgtggcggtttcggcattgagcgccggggtgatcacgcgcaatacgacgctgtttgacccaggctggtggcaactgccaggttcggaaaaacgttatcgtgactggaaaaaatggggccacgggcgtctgaatgtcacaagatcgctggaagaatctgcggataccttcttctatcaggtggcctacgatatggggatcgatcgcctctccgaatggatgggtaaattcggttatggtcattacaccggtatcgacctggcggaagaacgttccggcaacatgcctacccgcgaatggaaacagaaacgctttaaaaaaccgtggtatcagggtgacaccattccggttggtatcggtcagggttactggacagcgaccccaatccagatgagtaaggcactgatgatcctgattaatgacggtatcgtgaaggttcctcatttgctgatgagcaccgccgaagacggcaaacaggtgccatgggtacagccgcatgaaccgcccgtcggcgatattcattccggttactgggagctggcgaaagacggtatgtacggtgttgctaaccgccctaacggtacggcgcataaatactttgctagcgcaccgtacaaaattgcggcgaaatccggtaccgctcaggtcttcggtctgaaagcgaacgaaacctataatgcgcacaaaattgccgagcgtttacgtgaccacaaactgatgaccgcctttgcgccatacaacaatccgcaagtggctgtcgccatgattctggagaacggtggtgcgggtccggcggttggtacactgatgcgccagatcctcgaccacattatgctgggtgataacaacaccgatctgcctgcggaaaatccagcggttgccgcagcggaggaccattaataatactagaggtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0030_sequence 1 attaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z