BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_I14032
1
P(Lac) IQ
promoter P(Lac) IQ
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pMAL-p2X
Released HQ 2013
Constitutive Promoter, High Transcription
false
true
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1028343
1
-35
range1028343
1
3
8
annotation1028344
1
-10
range1028344
1
26
31
annotation1028342
1
P(Lac) IQ
range1028342
1
1
37
BBa_J29035
1
BBa_J29035
RodA expression
2006-08-03T11:00:00Z
2015-08-31T04:08:45Z
E. coli
RodA expression
false
false
_59_
0
879
59
Not in stock
false
Lac promorter->RBS->rodA->terminator
false
Kayo Yasuno
component1893558
1
BBa_B0012
component1893553
1
BBa_B0030
component1893556
1
BBa_B0010
component1893555
1
BBa_J29028
component1893551
1
BBa_I14032
annotation1893558
1
BBa_B0012
range1893558
1
1279
1319
annotation1893553
1
BBa_B0030
range1893553
1
46
60
annotation1893555
1
BBa_J29028
range1893555
1
67
1182
annotation1893551
1
BBa_I14032
range1893551
1
1
37
annotation1893556
1
BBa_B0010
range1893556
1
1191
1270
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_J29028
1
BBa_J29028
rod shape-determining protein
2006-08-03T11:00:00Z
2015-08-31T04:08:45Z
cell envelope of E. coli.
RodA is a rod shape-determining protein.
false
false
_59_
0
879
59
Not in stock
false
I added a stop codon TAATAA.
false
Kayo Yasuno
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I14032_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J29028_sequence
1
atgacggataatccgaataaaaaaacattctgggataaagtccatctcgatcccacaatgctgctgatcttactggcattgctggtttacagcgccctggttatctggagcgccagcggtcaggatattggcatgatggagcgtaaaatcggccaaatcgcgatgggtctggtcatcatggtggtgatggcgcaaattcctccacgcgtttatgaaggctgggccccctatctctatatcatctgtattattttgctggtggcggtagatgctttcggtgccatctctaaaggtgctcaacgctggctggacctcggtattgttcgttttcagccgtcggaaattgccaaaatagccgtaccactgatggttgcgcgctttatcaaccgcgacgtttgcccgccatcgttgaagaacactggcatcgcgctggtgctgatatttatgcccacgctgctggtggctgcacagcctgacctgggaacatcaatcctcgttgcgctttccggtctgtttgtactgttcctctctggccttagctggcgtctgattggcgtcgcagtagtgctggtagcggcgttcattccgattctgtggttcttcctgatgcatgattaccagcgccagcgcgtaatgatgctcctggacccggaatcagacccactcggcgcgggctatcacattattcagtctaaaattgctattggctccggcggattacgcggcaaaggctggctgcacggcactcagtcacagcttgaatttctccccgaacgccatactgactttatcttcgcggtactggcggaagagctgggattagtgggcattctgattctgctcgctctctacattctgctgatcatgcgcgggctgtggatagccgccagagcgcaaaccacctttggtcgcgtcatggctggcggcttaatgctgatattattcgtttatgtcttcgtaaatattggtatggtaagcggtattctgccggttgtaggggttccgctcccactggtcagttatggaggatcggcgctaattgtgctgatggctgggttcgggattgtaatgtcaatccacacccacaggaaaatgttgtcgaaaagcgtgtaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J29035_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgacggataatccgaataaaaaaacattctgggataaagtccatctcgatcccacaatgctgctgatcttactggcattgctggtttacagcgccctggttatctggagcgccagcggtcaggatattggcatgatggagcgtaaaatcggccaaatcgcgatgggtctggtcatcatggtggtgatggcgcaaattcctccacgcgtttatgaaggctgggccccctatctctatatcatctgtattattttgctggtggcggtagatgctttcggtgccatctctaaaggtgctcaacgctggctggacctcggtattgttcgttttcagccgtcggaaattgccaaaatagccgtaccactgatggttgcgcgctttatcaaccgcgacgtttgcccgccatcgttgaagaacactggcatcgcgctggtgctgatatttatgcccacgctgctggtggctgcacagcctgacctgggaacatcaatcctcgttgcgctttccggtctgtttgtactgttcctctctggccttagctggcgtctgattggcgtcgcagtagtgctggtagcggcgttcattccgattctgtggttcttcctgatgcatgattaccagcgccagcgcgtaatgatgctcctggacccggaatcagacccactcggcgcgggctatcacattattcagtctaaaattgctattggctccggcggattacgcggcaaaggctggctgcacggcactcagtcacagcttgaatttctccccgaacgccatactgactttatcttcgcggtactggcggaagagctgggattagtgggcattctgattctgctcgctctctacattctgctgatcatgcgcgggctgtggatagccgccagagcgcaaaccacctttggtcgcgtcatggctggcggcttaatgctgatattattcgtttatgtcttcgtaaatattggtatggtaagcggtattctgccggttgtaggggttccgctcccactggtcagttatggaggatcggcgctaattgtgctgatggctgggttcgggattgtaatgtcaatccacacccacaggaaaatgttgtcgaaaagcgtgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z